From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1012 [new locus tag: SA_RS05760 ]
  • pan locus tag?: SAUPAN003429000
  • symbol: argF
  • pan gene symbol?: argF
  • synonym:
  • product: ornithine carbamoyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1012 [new locus tag: SA_RS05760 ]
  • symbol: argF
  • product: ornithine carbamoyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1145818..1146819
  • length: 1002
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAATTTACGAAACAGAAGTTTTTTAACTTTATTAGACTTTTCACGACAAGAGGTA
    GAATTCTTATTAACACTCTCCGAGGATTTAAAACGTGCTAAATATATTGGCACTGAAAAG
    CCTATGTTAAAAAATAAAAATATTGCACTGTTATTTGAAAAAGATTCTACAAGAACGCGA
    TGTGCATTTGAAGTTGCAGCGCATGATCAAGGTGCAAATGTAACTTATTTAGGCCCAACT
    GGATCACAAATGGGTAAAAAAGAAACAACTAAAGATACTGCACGTGTGCTTGGTGGAATG
    TATGATGGCATTGAATACCGTGGTTTTTCACAAAGAACAGTAGAAACTTTAGCTGAATAT
    TCAGGCGTACCAGTGTGGAATGGTTTAACTGATGAAGATCATCCTACTCAAGTTCTTGCT
    GATTTCTTAACAGCAAAAGAAGTCTTAAAAAAAGATTATGCAGATATTAACTTTACATAT
    GTTGGAGATGGTCGTAATAACGTTGCAAATGCATTAATGCAAGGTGCTGCCATTATGGGT
    ATGAACTTCCATTTAGTTTGTCCAAAAGAATTAAATCCAACAGATGAATTATTAAATCGC
    TGTAAAAATATTGCCGCTGAAAATGGTGGCAACATATTAATCACAGATGATATTGACCAA
    GGTGTAAAAGGTTCGGATGTAATTTACACTGATGTTTGGGTATCAATGGGTGAACCTGAT
    GAAGTATGGAAAGAACGACTTGAATTATTGAAACCATATCAAGTAAATAAAGAAATGATG
    GATAAAACTGGTAATCCAAATGTTATTTTTGAGCATTGCTTACCATCTTTCCATAATGCT
    GATACGAAAATTGGTCAACAAATTTTTGAAAAATATGGTATTCGAGAAATGGAAGTTACA
    GATGAAGTATTCGAAAGTAAAGCTTCAGTTGTATTCCAAGAAGCTGAGAACAGAATGCAT
    ACAATCAAAGCAGTCATGGTTGCTACATTGGGTGAATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1012 [new locus tag: SA_RS05760 ]
  • symbol: ArgF
  • description: ornithine carbamoyltransferase
  • length: 333
  • theoretical pI: 4.81544
  • theoretical MW: 37534.4
  • GRAVY: -0.405405

Function[edit source | edit]

  • reaction:
    EC 2.1.3.3?  ExPASy
    Ornithine carbamoyltransferaseCarbamoyl phosphate + L-ornithine = phosphate + L-citrulline
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 408.5)
    MetabolismEnergy metabolismAmino acids and aminesputrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 268.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisaspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 120.5)
    knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 65)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Ornithine carbamoyltransferase (EC 2.1.3.3) 
    Arginine; urea cycle, polyaminesArginine Biosynthesis extended Ornithine carbamoyltransferase (EC 2.1.3.3) 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Ornithine carbamoyltransferase (EC 2.1.3.3) 
  • PFAM:
    Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 193)
    no clan definedOTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 152.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.172
    • Ymax_pos: 11
    • Cmax: 0.112
    • Cmax_pos: 36
    • Smax: 0.413
    • Smax_pos: 2
    • Smean: 0.282
    • D: 0.215
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)