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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1463 [new locus tag: SA_RS08245 ]
  • pan locus tag?: SAUPAN004243000
  • symbol: secF
  • pan gene symbol?: secDF
  • synonym:
  • product: preprotein translocase subunit SecD/SecF

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1463 [new locus tag: SA_RS08245 ]
  • symbol: secF
  • product: preprotein translocase subunit SecD/SecF
  • replicon: chromosome
  • strand: -
  • coordinates: 1671270..1673549
  • length: 2280
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    GTGAAGAAAAGTAGTAGAATAATTGCGTTCTTGCTTTTAGTCGTATTGTTGTTTGCCGGA
    ATGGCTGCAACTTATAAAAGTGTTATAAAGAATGTCAATTTAGGACTTGATTTGCAAGGT
    GGATTTGAGGTGCTTTATCAAGTCGATCCTTTAAATAAAGGCGACAAAATAGATAAAAAA
    GCCTTACAATCAACAGCGCAAACATTAGAAAATCGTGTTAACGTTTTAGGTGTTTCGGAA
    CCTAAAATTCAAGTTGAAGAACCTAATAGAATTAGAGTTCAACTTGCTGGTGTAACAGAC
    CAGAATGAAGCACGTAAAATTTTATCTTCTCAAGCTAATTTAACAATTCGCGATGCAGAA
    GATAAAGTAAAATTAAGCGGTTCTGATATTAAACAGGGATCTGCCAAACAAGAATTTAAA
    CAGGAAACAAACCAGCCTACAGTTACTTTTAAAGTAAAAGACAAAAACAAATTTAAAAAA
    GTAACGGAAGAAATCTCTAAGAAACGTGATAATGTCATGGTTGTTTGGTTAGATTTCAAA
    AAAGGTGACAGCTATAAAAAAGAAGCACAAAAGAAAAACCCTAAATTTATTTCTGCAGCA
    AGTGTTGATCAACCAATCAATTCTGATAGCGTAGAAATCTCGGGTGGATTTAAAGGACAA
    GAAGGCGTTAAAAAAGCAAAACAAATCGCCGAATTGTTAAATGCAGGTTCTTTACCAGTT
    GACTTGAAAGAAATTTATTCAAACTCAGTAGGTGCCCAATTTGGTCAAGATGCATTAGAT
    AAAACTGTGTTTGCATCGTTTATTGGTGTAGCATTAATTTATTTATTCATGCTTGGATTC
    TACCGATTACCTGGTTTAGTAGCGATTATTGCATTGACAACTTATATCTATCTAACGTTA
    GTAGCATTTAATTTCATTTCCGGGGTTCTAACTTTACCAGGATTAGCGGCGTTGGTATTA
    GGTGTAGGTATGGCTGTAGATGCCAATATTATCATGTATGAGCGTATTAAGGATGAACTT
    CGAATAGGTAGAACGATAAAGCAAGCCTTTTCTAAAGCAAACAAAAGTTCATTCCTAACA
    ATTTTTGATTCTAACTTAACAACAGTTATCGCCGCAGCAGTATTATTCTTCTTCGGTGAA
    AGTTCAGTTAAAGGTTTCGCGACAATGTTATTATTAGGTATTCTAATGATCTTTGTTACA
    GCCGTGTTCTTATCAAGATTCTTATTATCATTACTTGTTTCATCAAATATATTCAAAAAT
    CAATTTTGGTTATTTGGTGTTAAAAAGAATAAACGACATGATATTAATGAAGGTGTAGAT
    GTTCATGACCTTAAAACTTCATTCGAGAAATGGAATTTTGTTAAATTAGCTAAGCCATTA
    ATTGGAGTAAGTATTTTAATTGTAGTTGTCGGTTTAGTTATTCTTTATATCTTCAAGTTA
    AACTTAGGAATTGATTTCTCAAGTGGTACACGTGTAGATTTCCAATCTAAACAAGCAATT
    ACTCAACAAAAGGTTGAGCAAGTTGTAAAAGATAGTGGACTTAAAGCGGATCAAATTCAA
    ATCAATGGTAAAGATAATAAAGTAGCAACTGTTCAATTTAAAGACGATTTAACGCGTGCT
    CAAGATAATAAATTGAGTGATAATATTAAATCGAAATTCGGTGACACACCTCAAATTAAT
    ACTGTATCACCAATTATTGGACAGGAATTAGCAAAAAATGCAATGCTTGCATTAATCTAT
    GCATCGATAGGTATCATCATCTATGTATCATTACGATTTGAATGGCGCATGGGTCTTTCA
    TCTGTATTGGCATTATTACATGATGTATTCATTATAGTAGCGATTTTCAGTTTATTTAGA
    ATTGAAGTAGATTTAACATTTATCGCCGCTGTATTAACAATTGTCGGTTATTCAATTAAT
    GATACAATCGTAACGTTTGACCGTGTACGTGAAAACTTACAAAAGGTTAAAGTGATTACG
    ACAACAGAACAAATTGATGATATCGTTAATAGATCAATTAGACAGACAATGACACGTTCA
    ATTAATACAGTATTAACAGTTATTGTAGTAGTAGTTGCTATACTATTCTTCGGTGCTCCT
    ACGATATTCAACTTTACTTTAGCATTATTTATCGGATTGATTTCTGGTGTATTCTCTTCA
    ATCTTCATTGCCGTTCCGCTATGGGGAATAATGAAAAAACGTCAGTTGAAAAAATCGCCG
    AAACACAAATTAGTTGTATATAAAGAAAAGAAATCGAACGATGAAAAGATTTTAGTTTAA
    60
    120
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    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
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    1260
    1320
    1380
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    1560
    1620
    1680
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    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1463 [new locus tag: SA_RS08245 ]
  • symbol: SecF
  • description: preprotein translocase subunit SecD/SecF
  • length: 759
  • theoretical pI: 10.3191
  • theoretical MW: 84253.3
  • GRAVY: 0.234124

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingprotein-export membrane protein SecD (TIGR01129; HMM-score: 386.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingprotein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 338.2)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingprotein-export membrane protein SecF (TIGR00966; HMM-score: 276.2)
    MetabolismTransport and binding proteinsUnknown substrateefflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 58.2)
    MetabolismTransport and binding proteinsUnknown substratetransport protein (TIGR00833; HMM-score: 27.2)
  • TheSEED:  
    Protein translocase subunit SecD / Protein translocase subunit SecF 
  • PFAM:
    RND_permease (CL0322) SecD_SecF; Protein export membrane protein (PF02355; HMM-score: 256)
    no clan definedSec_GG; SecD/SecF GG Motif (PF07549; HMM-score: 39.6)
    RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 39.2)
    no clan definedSelenoprotein_S; Selenoprotein S (SelS) (PF06936; HMM-score: 12.7)
    POTRA (CL0191) POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 12.4)
    no clan definedSecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 11.4)
    BRCT-like (CL0459) RTT107_BRCT_6; Regulator of Ty1 transposition protein 107 BRCT domain (PF16771; HMM-score: 8.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 23 aa
    • Ymax: 0.323
    • Ymax_pos: 23
    • Cmax: 0.171
    • Cmax_pos: 23
    • Smax: 0.847
    • Smax_pos: 6
    • Smean: 0.693
    • D: 0.467
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

  • GI: 15927217 NCBI
  • RefSeq: NP_374750 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEVLYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQNEARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKKVTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFKGQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLFMLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYERIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLLGILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEKWNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQVVKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPIIGQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVDLTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSINTVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSPKHKLVVYKEKKSNDEKILV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]