From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1465 [new locus tag: SA_RS08255 ]
  • pan locus tag?: SAUPAN004245000
  • symbol: tgt
  • pan gene symbol?: tgt
  • synonym:
  • product: queuine tRNA-ribosyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1465 [new locus tag: SA_RS08255 ]
  • symbol: tgt
  • product: queuine tRNA-ribosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1674103..1675242
  • length: 1140
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAGGT
    ATCGTGCACACACCACACGGTTCATTTGAAACACCTATGTTTATGCCAGTTGGTACTAAA
    GCAACCGTTAAAACAATGAGTCCAGAAGAGTTAAGACAAATTGAAGCAAAAATCATTTTG
    GGCAACACATATCATTTGTGGTTACAACCCGGAAATGATATTATCAAACACGCTGGGGGA
    TTACATAAATTCATGAATTGGGATGGTCCGATTCTTACAGATTCAGGCGGTTTCCAAGTG
    TTTAGTTTAAGTAATTTACGTAAAATTACAGAAGAAGGCGTGGAATTTAGACATCATACT
    AATGGGTCTAAATTATTTTTGAGTCCTGAGAAATCAATGCAAATTCAAAATGATTTAGGA
    TCTGATATTATGATGGCATTTGATGAATGTCCACCGATGCCTGCTGAATATGATTATGTA
    AAAAAATCTATTGAACGTACAACACGTTGGGCGAAAAGATGTCTAGATGCACACCAAAGA
    CCTGAAGATCAAGCATTGTTCGGCATTATACAAGGTGGCGAATATGAAGATTTAAGAGAA
    CAAAGTGCAAAGGATTTAGTAGAATTAGATTTTCCTGGTTATGCAATCGGCGGTTTGTCA
    GTTGGTGAACCTAAACCGGTTATGTATAAAATGGTTGAACATACAGAGCAGTTTATGCCT
    AAAGATAAACCAAGATATTTAATGGGTGTAGGATCTCCAGATGCGTTAATCGAATGTAGT
    ATTCGCGGCATGGATATGTTTGATTGTGTCTTACCGACACGTATTGCCAGAAATGGTACT
    TGTATGACATCGCAAGGTCGTTTAGTTATTAAAAATGCAAAATTTGCAGATGATTTAAGA
    CCGTTAGATGAGAATTGTGACTGTTATACATGTCAAAACTATTCAAGAGCGTATATACGT
    CATTTAATCAAGGCAGAGGAAACTTTTGGTATTCGTCTTACTACTATTCATAATTTACAT
    TTTCTGCTAAAATTAATGGAAGATATAAGACAAGCCATTCGAGAAGATCGTCTTTTAGAT
    TTCAAAGAAGAATTCTTCGAGCAATATGGATTAAATGTTGAGAACCCAAAAAACTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1465 [new locus tag: SA_RS08255 ]
  • symbol: Tgt
  • description: queuine tRNA-ribosyltransferase
  • length: 379
  • theoretical pI: 6.3237
  • theoretical MW: 43309.5
  • GRAVY: -0.435356

Function[edit source | edit]

  • reaction:
    EC 2.4.2.29?  ExPASy
    tRNA-guanine34 transglycosylaseGuanine34 in tRNA + queuine = queuosine34 in tRNA + guanineGuanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA-guanine transglycosylase (TIGR00430; EC 2.4.2.29; HMM-score: 607.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA-guanine family transglycosylase (TIGR00449; EC 2.4.2.-; HMM-score: 575.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA-guanine(15) transglycosylase (TIGR00432; EC 2.4.2.48; HMM-score: 121.7)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationQueuosine-Archaeosine Biosynthesis tRNA guanosine(34) transglycosylase (EC 2.4.2.29) 
  • PFAM:
    no clan definedTGT; Queuine tRNA-ribosyltransferase (PF01702; HMM-score: 319.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 18
    • Cmax: 0.137
    • Cmax_pos: 31
    • Smax: 0.25
    • Smax_pos: 8
    • Smean: 0.202
    • D: 0.169
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]