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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1488 [new locus tag: SA_RS08375 ]
  • pan locus tag?: SAUPAN004275000
  • symbol: valS
  • pan gene symbol?: valS
  • synonym:
  • product: valyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1488 [new locus tag: SA_RS08375 ]
  • symbol: valS
  • product: valyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1693943..1696573
  • length: 2631
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAATGAAACCAAAATATGATCCTCGTGAAGTTGAAGCGGGACGTTATGAAGAATGG
    GTAAAGAATGGTTATTTTAAACCGTCAGAAGATAAATCAAAAGAAACATATACAATTGTT
    ATCCCGCCACCAAATGTAACTGGTAAATTACATTTAGGACATGCATGGGATACGACTTTA
    CAAGATATCATTACACGTATGAAACGTATGCAAGGATACGATACGTTATACTTACCAGGT
    ATGGATCATGCTGGTATTGCGACACAGGCAAAGGTAGAAGCTAAATTAAATGAACAAGGA
    ATAACTAGATATGATCTTGGTCGTGAAAAGTTTTTAGAACAGGCATGGGATTGGAAAGAA
    GAGTATGCGTCATTTATTCGTGCGCAATGGGCTAAATTAGGTCTAGGTTTAGATTATAGT
    AGAGAACGTTTTACTTTAGATGAAGGTTTAAGTAAAGCAGTTAAAAAAGTTTTTGTTGAT
    TTATACAATAAAGGAATTATTTATCGTGGCGAACGTATTATAAATTGGGATCCTAAAGCA
    CGTACAGCTTTATCTGATATTGAAGTAATACATGAAGATGTTCAAGGTGCGTTTTATCAT
    TTTAAATATCCTTACGCTGATGGTGAAGGTTTTATTGAAATTGCAACAACAAGACCAGAA
    ACGATGTTAGGTGATACAGCGATTGTTGTTAACCCTAATGACGAACGATACAAAGATGTA
    ATCGGTAAAACTGTTATATTACCAATCGTAGGACGCGAACTGCCTATTTTAGCAGATGAG
    TATGTTGATATAGACTTCGGTTCTGGTGCTATGAAAGTGACACCAGCACATGACCCTAAT
    GATTTTGAAATTGGTCAAAGACATCAATTAGAAAATATTATCGTTATGGATGAAAATGGT
    AAAATGAACGACAAAGCGGGTAAATATGAAGGTATGGACCGTTTTGATTGTCGTAAACAG
    CTAGTTAAAGATTTAAAAGAACAAGATTTAGTTATCAAGATTGAAGATCATGTTCATTCT
    GTAGGTCATTCAGAACGATCTGGCGCTGTTGTTGAACCATATTTATCAACACAATGGTTT
    GTGCGCATGGAAGACTTAGCGAAACGTTCATTAGATAACCAAAAAACAGATGATCGTATT
    GATTTTTATCCGCAACGTTTCGAACATACATTTAACCAATGGATGGAAAATATTAGAGAT
    TGGACGATTTCAAGACAATTATGGTGGGGTCATCAAATTCCGGCTTGGTATCATAAAGAA
    ACAGGCGAAATATATGTTGGAGAAGAAGCGCCAACTGATATTGAAAATTGGCAACAAGAT
    GAAGATGTATTAGATACGTGGTTCTCAAGTGCTTTATGGCCTTTCTCTACGTTAGGTTGG
    CCTGATTTAGAAAGTGAAGACTTTAAACGATACTACCCAACAAATGCCTTAGTTACAGGT
    TACGATATTATCTTTTTCTGGGTCGCACGCATGATATTCCAAGGCTTAGAATTTACAGAT
    CGTCGTCCATTTAATGATGTATTATTACACGGTTTAGTTCGTGCTGAAGACGGGCGTAAG
    ATGAGTAAATCATTAGGTAATGGTGTGGATCCAATGGATGTTATTGACGAATACGGTGCT
    GATAGCTTGCGTTACTTCTTAGCAACAGGTTCATCTCCAGGACATGATTTAAGATACTCA
    ACTGAAAAAGTTGAGTCAGTGTGGAACTTTATCAATAAAATCTGGAATGGGGCACGTTTC
    AGTTTAATGAATATCGGTGAAGACTTTAAAGTTGAAGATATCGATTTAAGTGGTAACTTA
    TCATTAGCAGATAAATGGATTCTAACACGTTTAAATGAAACGATTGCAACAGTTACTGAT
    TTAAGTGACAAATATGAATTCGGCGAAGTTGGACGTGCATTATATAATTTCATTTGGGAT
    GATTTCTGTGATTGGTACATTGAAATGAGTAAAATTCCAATGAATAGTAATGATGAAGAA
    CAAAAACAAGTTACACGTTCAGTATTGAGTTATACTTTAGACAATATTATGAGAATGCTA
    CATCCATTCATGCCATTTGTAACAGAGAAAATATGGCAAAGTTTACCACATGAAGGTGAC
    ACAATTGTTAAAGCTTCATGGCCAGAAGTGCGTGAATCATTGATTTTTGAAGAAAGTAAA
    CAAACAATGCAACAACTTGTTGAAATCATTAAATCTGTAAGACAATCACGTGTAGAAGTA
    AATACGCCATTGTCTAAAGAAATACCTATTTTAATTCAAGCTAAAGATAAAGAAATTGAA
    ACAACTTTATCACAAAACAAAGATTATTTAATCAAATTCTGTAATCCTAGTACCTTAAAT
    ATTAGCACTGACGTGGAAATTCCTGAGAAAGCAATGACATCAGTTGTAATTGCAGGTAAA
    GTGGTATTACCATTAGAAGGGCTAATTGATATGGATAAGGAAATCAGCCGTTTGGAAAAA
    GAATTAGCTAAACTTCAAAGCGAATTAGATAGAGTAGATAAAAAGCTCTCTAATGAAAAC
    TTTGTAAGTAAAGCACCTGAAAAGGTTATAAATGAAGAAAAACGTAAAAAACAAGATTAT
    CAAGAAAAATATGATGGTGTGAAGGCAAGAATTGAACAATTAAAAGCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2631

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1488 [new locus tag: SA_RS08375 ]
  • symbol: ValS
  • description: valyl-tRNA synthetase
  • length: 876
  • theoretical pI: 4.7353
  • theoretical MW: 101723
  • GRAVY: -0.560731

Function[edit source | edit]

  • reaction:
    EC 6.1.1.9?  ExPASy
    Valine--tRNA ligaseATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 1183.3)
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 424.5)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 200.1)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 197.3)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 134.1)
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 31.4)
    Genetic information processingProtein synthesistRNA aminoacylationarginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 16.6)
  • TheSEED:  
    Valyl-tRNA synthetase (EC 6.1.1.9) 
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 723.5)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 144.4)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 108.8)
    tRNA_bind_arm (CL0298) Val_tRNA-synt_C; Valyl tRNA synthetase tRNA binding arm (PF10458; HMM-score: 82.6)
    HUP (CL0039) tRNA-synt_1_2; Leucyl-tRNA synthetase, Domain 2 (PF13603; HMM-score: 41.8)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 34.8)
    no clan definedCCDC-167; Coiled-coil domain-containing protein 167 (PF15188; HMM-score: 15.1)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 14.4)
    tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 13.3)
    no clan definedDUF2408; Protein of unknown function (DUF2408) (PF10303; HMM-score: 13.3)
    ABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 13.1)
    DASH_Spc19; Spc19 (PF08287; HMM-score: 11.3)
    DUF5082; Domain of unknown function (DUF5082) (PF16888; HMM-score: 10.8)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 9.1)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 8.2)
    Vps51 (CL0295) COG2; COG (conserved oligomeric Golgi) complex component, COG2 (PF06148; HMM-score: 7.7)
    no clan definedIncA; IncA protein (PF04156; HMM-score: 6.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA0727(gap)glyceraldehyde-3-phosphate dehydrogenase  [2] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 11
    • Cmax: 0.105
    • Cmax_pos: 49
    • Smax: 0.144
    • Smax_pos: 1
    • Smean: 0.093
    • D: 0.097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPPNVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGITRYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVDLYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTRPETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPAHDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKIEDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFNQWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSSALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVLLHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVESVWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDKYEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHPFMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEVNTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIAGKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRKKQDYQEKYDGVKARIEQLKA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: T-box(Val)
    T-box(Val) (RNA) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)
  2. 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]