From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1527 [new locus tag: SA_RS08595 ]
  • pan locus tag?: SAUPAN004354000
  • symbol: SA1527
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1527 [new locus tag: SA_RS08595 ]
  • symbol: SA1527
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1745419..1746717
  • length: 1299
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAAAACATGAACAAATTTTAGATTATATTGAATCGTTATCTATAGGCTCTAAAATT
    TCGGTGCGTAAAATCGCTAAATTTTTAAACGTATCTGAAGGGACAGCATATCGTGCAATT
    AAAGATGCTGATAAAATGGGCATGGTCGCAACGATAGATCGTGTTGGTACGGTTCGAATC
    GAAAAACGTAATCGTAATGAAATTGAACATTTAACTTTTAATGAAATTGTCAATATTATT
    GATGGCCAAGTGTTAGGTGGCAATAAAGGTATCACTAAGATGGTATCTAAATTTGCAATT
    GGAGCAATGGAACTCAAAGATATTTTAAAATATATTGGTCCAAAGACATTATTGATTGTC
    GGAAATCGCGAAGATGTGCAGATTGAAGCTTTAAAAAGAGGTACGGCTATATTAATTACT
    GGTGGATTTAAGCCATCTAATAAAGTTATTAATTTTGCGAATGAACATGATTTACCTGTT
    CTATCATCAAGTTATGATACATTCCTAGTTGCAAATATTATCAACAAAGCACTTTTTAAT
    CAGAAAATACGAAAAGATATCTTAATAGTTCAAGATATAATGACGCCATTAGATGATTTG
    TCTGTGCTGTTTGATACGATGAAAATAGCAGATTATAAGCGTATGGCGAATCGAACTGGC
    CATACAAGATTTCCGGTAGTCAATGAGTCGTATAAATTAGTAGGTATTGTAACAAGTCGA
    GAGATGATTAATACAAAGGACGACGACGAAATAGATAAAGTGATGACGCGAAACCCTATT
    TATGTAAATGCTATGAGTACAGTAGCAAGTTGTGCGCATATGATGATTTGGGAAGGTATT
    GAATTGATTCCTGTCGTTTCGAGTAATAAAAAGACAGTGGGTGTGATTAATAGACAAGAT
    GTTTTAAAGTCTATGCAACTTTTAGGAAGACAGCCTCAGATGGGTGAAACAATTAACGAT
    CAAATCGCCAAATATATAACAATGAATCAAGATGGAATTACAGTAGAAGTTTCACCACTT
    TTAATCAATCATTACGGAACAGTAAGTAAAGCGGCATTTGTAAGTATTATAGAAGAAACA
    ATACAATATGAAATGCGTAAATTTAAAAAAGGTAATGTCATGATAGAGAACTTAAATATC
    GTGTATATTAAAACAGTACCTATTGAATCACATATAACAGTGCGCTTTGGCATACTAGAC
    GTCGGAAGAAACTTCGCCAAAATAGAAGTGAACATGCATAGTCAAAACGACAAAGTAGCG
    AGCGCTCTCGTGATATGCCAAATGTTTGACGAAGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1299

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1527 [new locus tag: SA_RS08595 ]
  • symbol: SA1527
  • description: hypothetical protein
  • length: 432
  • theoretical pI: 9.09376
  • theoretical MW: 48530.3
  • GRAVY: -0.0368056

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisinosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 42.1)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmagnesium transporter (TIGR00400; HMM-score: 37.2)
    MetabolismAmino acid biosynthesisSerine familycystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 25.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 21.6)
    Signal transductionRegulatory functionsDNA interactionsCRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 19.4)
    MetabolismEnergy metabolismSugarssugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 18.9)
    Unknown functionGeneralDNA binding domain, excisionase family (TIGR01764; HMM-score: 12.5)
  • TheSEED:  
    Uncharacterized protein YtoI 
  • PFAM:
    MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 102)
    no clan definedCBS; CBS domain (PF00571; HMM-score: 49.3)
    HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 22.9)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 21.9)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.7)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 21.6)
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 19.6)
    no clan definedPucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 19.4)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 18.2)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 15.5)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 15.5)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 14.9)
    HTH_22; HTH domain (PF13309; HMM-score: 14.3)
    HotDog (CL0050) 4HBT_2; Thioesterase-like superfamily (PF13279; HMM-score: 14.3)
    HTH (CL0123) HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 13.6)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.8)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.128
    • Ymax_pos: 39
    • Cmax: 0.125
    • Cmax_pos: 39
    • Smax: 0.17
    • Smax_pos: 24
    • Smean: 0.123
    • D: 0.126
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDADKMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGAMELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPVLSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANRTGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMIWEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGITVEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHITVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]