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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1608 [new locus tag: SA_RS09055 ]
  • pan locus tag?: SAUPAN004501000
  • symbol: metK
  • pan gene symbol?: metK
  • synonym:
  • product: S-adenosylmethionine synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1608 [new locus tag: SA_RS09055 ]
  • symbol: metK
  • product: S-adenosylmethionine synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1843215..1844411
  • length: 1197
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAA
    ATCGCTGACCAAGTGTCAGATGCAATATTAGATGCTATTTTAAAAGACGACCCAAATGCA
    CGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCATTGATCGCCGGCGAAATTTCT
    ACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
    ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAA
    CAATCACCTGACATTGCACAAGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGC
    GAAGAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTTTAATGTTTGGTTACGCAACG
    AATGAAACAGAAACGTACATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGT
    TTATCTGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAG
    GTCACTGTTGAATATGATGAAAATGATAACCCAGTACGTATTGATACGATTGTCGTATCA
    ACACAACATGCTGATGATGTGACACTTGAACAAATTCAAGAAGACATCAAAGCGCATGTC
    ATTTATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCT
    ACAGGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATC
    ATTGTTGATACGTATGGTGGCTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAGGAT
    CCTACAAAAGTAGACCGCTCAGCTGCATATGCTGCACGTTATGTGGCTAAAAATATTGTT
    GCAGCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAA
    CCAGTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGACAACTTGTC
    GAAGCAGTTAGAAAACACTTTGACCTAAGACCTGCAGGCATTATTAAAATGCTTGATTTG
    AAACAACCGATTTACAAACAAACTGCTGCTTATGGTCATTTTGGACGTACAGATGTATTA
    TTCCCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1197

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1608 [new locus tag: SA_RS09055 ]
  • symbol: MetK
  • description: S-adenosylmethionine synthetase
  • length: 398
  • theoretical pI: 4.61692
  • theoretical MW: 43790
  • GRAVY: -0.327889

Function[edit source | edit]

  • reaction:
    EC 2.5.1.6?  ExPASy
    Methionine adenosyltransferaseATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOthermethionine adenosyltransferase (TIGR01034; EC 2.5.1.6; HMM-score: 621.1)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis S-adenosylmethionine synthetase (EC 2.5.1.6) 
    Lysine, threonine, methionine, and cysteineMethionine Degradation S-adenosylmethionine synthetase (EC 2.5.1.6) 
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis S-adenosylmethionine synthetase (EC 2.5.1.6) 
  • PFAM:
    no clan definedS-AdoMet_synt_C; S-adenosylmethionine synthetase, C-terminal domain (PF02773; HMM-score: 238.1)
    S-AdoMet_synt_M; S-adenosylmethionine synthetase, central domain (PF02772; HMM-score: 162.2)
    S-AdoMet_synt_N; S-adenosylmethionine synthetase, N-terminal domain (PF00438; HMM-score: 145.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Co2+, K+, Mg2+
  • effectors:
  • protein partners:
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA1532hypothetical protein  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 11
    • Cmax: 0.14
    • Cmax_pos: 23
    • Smax: 0.352
    • Smax_pos: 1
    • Smean: 0.211
    • D: 0.177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAM
    SAM (RNA) important in Methionine biosynthesisRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)
  2. 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]