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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1862 [new locus tag: SA_RS10690 ]
  • pan locus tag?: SAUPAN005307000
  • symbol: leuA
  • pan gene symbol?: leuA
  • synonym:
  • product: 2-isopropylmalate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1862 [new locus tag: SA_RS10690 ]
  • symbol: leuA
  • product: 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2102502..2104031
  • length: 1530
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTAGTCATATTCAAATTTTTGATACGACACTAAGAGACGGTGAACAAACACCAGGA
    GTGAATTTTACTTTTGATGAACGCTTGCGTATTGCATTGCAATTAGAAAAATGGGGTGTA
    GATGTTATTGAAGCTGGATTTCCTGCTTCAAGTACAGGTAGCTTTAAATCTGTTCAAGCA
    ATTGCACAAACATTAACAACAACGGCTGTATGTGGTTTAGCTAGATGTAAAAAATCTGAC
    ATCGATGCTGTATATGAAGCAACAAAAGATGCAGCGAAGCCGGTCGTGCATGTTTTTATA
    GCAACATCACCTATTCATCTTGAACATAAACTTAAAATGTCTCAAGAAGACGTTTTAGCA
    TCTATTAAAGAACATGTCACATACGCGAAACAATTATTTGACGTTGTTCAATTTTCACCT
    GAAGATGCAACGCGTACTGAATTACCATTCTTAGTGAAATGTGTACAAACTGCCGTTGAC
    GCTGGAGCTACAGTTATTAATATTCCTGATACAGTCGGCTACAGTTACCATGATGAATAT
    GCACATATTTTCAAAACCTTAACAGAATCTGTAACATCTTCAAATGAAATTATTTATAGT
    GCTCATTGCCATGACGATTTAGGAATGGCTGTTTCAAATAGTTTAGCTGCAATTGAAGGC
    GGTGCGAGACGAATTGAAGGCACTGTAAATGGTATTGGTGAACGAGCAGGTAATGCAGCA
    CTTGAAGAAGTCGCGCTTGCACTATACGTTCGAAATGATCATTATGGTGCTCAAACTGCC
    CTTAATCTCGAAGAAACTAAAAAAACATCGGATTTAATTTCAAGATATGCAGGTATTCGA
    GTGCCTAGAAATAAAGCAATTGTTGGCCAAAATGCATTTAGTCATGAATCAGGTATTCAC
    CAAGATGGCGTATTAAAACATCGTGAAACATATGAAATTATGACACCTCAACTTGTTGGT
    GTAAGCACGACTGAACTTCCATTAGGAAAATTATCTGGTAAACACGCCTTCTCAGAGAAG
    TTAAAAGCATTAGGTTATAACATTGATAAAGAAGCGCAAATAGATTTATTTAAACAATTC
    AAGACCATTGCGGACAAAAAGAAATCTGTTTCAGATAGAGATATTCATGCGATTATTCAA
    GGTTCTGAGCATGAGCATCAAGCACTTTATAAATTGGAAACACTACAACTACAATATGTC
    TCTAGCGGCCTTCAAAGTGCTGTTGTTGTTGTTAAAGATAAAGAGGGTCATATTTACCAG
    GATTCAAGTATTGGTACTGGTTCAATCGTAGCAATTTACAATGCAGTTGATCGTATTTTC
    CAGAAAGAAACAGAATTAATTGATTATCGTATTAATTCTGTCACTGAAGGTACTGATGCC
    CAAGCAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATT
    GATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCA
    GCTGAAAATGTTGAGAAGGTAGGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1862 [new locus tag: SA_RS10690 ]
  • symbol: LeuA
  • description: 2-isopropylmalate synthase
  • length: 509
  • theoretical pI: 5.86528
  • theoretical MW: 55703.4
  • GRAVY: -0.203733

Function[edit source | edit]

  • reaction:
    EC 2.3.3.13?  ExPASy
    2-isopropylmalate synthaseAcetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 662.9)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 404.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315)
    MetabolismCentral intermediary metabolismNitrogen fixationhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 283.5)
    Unknown functionGeneralcitramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 208.1)
    MetabolismAmino acid biosynthesisPyruvate family2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 126.9)
    MetabolismEnergy metabolismOther4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 75.6)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsoxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
    MetabolismEnergy metabolismOtheroxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsBranched-Chain Amino Acid Biosynthesis 2-isopropylmalate synthase (EC 2.3.3.13) 
    Branched-chain amino acidsLeucine Biosynthesis 2-isopropylmalate synthase (EC 2.3.3.13) 
  • PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 311.9)
    no clan definedLeuA_dimer; LeuA allosteric (dimerisation) domain (PF08502; HMM-score: 122.5)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 10.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 63
    • Cmax: 0.142
    • Cmax_pos: 63
    • Smax: 0.14
    • Smax_pos: 60
    • Smean: 0.083
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVGN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]