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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2071 [new locus tag: SA_RS11910 ]
  • pan locus tag?: SAUPAN005737000
  • symbol: moeB
  • pan gene symbol?: moeB
  • synonym:
  • product: molybdopterin biosynthesis protein moeB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2071 [new locus tag: SA_RS11910 ]
  • symbol: moeB
  • product: molybdopterin biosynthesis protein moeB
  • replicon: chromosome
  • strand: -
  • coordinates: 2331836..2332840
  • length: 1005
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAGTCAAGAACGTTATTCAAGGCAAATTTTATTTAAACAAATAGGTGAAATAGGTCAA
    AGCAAAATAAATCAAAAATGTGCGTTGATTATTGGTATGGGCGCATTAGGTACACATGTG
    GCCGAAGGACTTGTTAGAGCAGGCATTGCTAAACTAATCATTGTTGATAGAGATTATATT
    GAATTTAGTAATTTACAAAGACAAACATTGTTTACTGAAGAAGATGCTTTGAAAATGATG
    CCTAAAGTGGTTGCAGCTAAAAAGCATTTGCTAGCGTTACGTAGTGATGTTGATATTGAT
    GGTTGTATTGCCCATGTGGATTATTATTTTTTGGAAACACATGGACAGGACGTTGACGTT
    ATTATTGATGCAACCGATAACTTTGAAACACGACAACTGATTAATGATTTTGCATATAAA
    CATCGTATACCTTGGATTTATGGTGGTGTTGTACAGAGTACATATACAGAAGCTGCATTT
    ATACCTGGTAAAACACCTTGCTTTAACTGTTTGGTACCACAATTGCCAGCATTAAATTTA
    ACATGTGATACAGTAGGGGTCATTCAACCTGCCGTGACGATGGCAACAAGTTTACAATTA
    AGAGATGCGATGAAAGTATTAACGGAACAACCAATTGACACAAAAATAACTTATGGCGAT
    ATTTGGGAAGGTAGTCATTATTCATTTGGTTTCAGTAAAATGCAACGTTCAGACTGTACA
    ACTTGTGGAGATGTACCAAGTTATCCGTATTTAAACAAGAATGAACAACGTTATGCAACA
    TTGTGTGGTAGAGACACTGTACAGTATGAAAATGCATCAATTACACACGACATTCTTGTT
    CAATTTTTAAAACAACATCAACTAAATTATCGCAGTAATTCGTATATGGTTATGTTTGAA
    TTTAAAGGACACCGCATTGTTGCTTTTAAAGGTGGAAGGTTTTTAATACATGGCATGACA
    CGCACATCAGATGCCACACATCTAATGAATTTATTGTTTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2071 [new locus tag: SA_RS11910 ]
  • symbol: MoeB
  • description: molybdopterin biosynthesis protein moeB
  • length: 334
  • theoretical pI: 7.0759
  • theoretical MW: 37776.2
  • GRAVY: -0.12994

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 205.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinmolybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 126.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 67.2)
    ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 36.9)
    E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 32.9)
    Cellular processesCellular processesPathogenesisthiazole/oxazole-forming peptide maturase, SagC family component (TIGR03603; EC 6.3.-.-; HMM-score: 29.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 20.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme A2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.1)
  • TheSEED:  
    Molybdenum cofactor biosynthesis Molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) 
  • PFAM:
    NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 169.9)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 23.9)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.6)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 16.1)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 14.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 13.9)
    KR; KR domain (PF08659; HMM-score: 13)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 41
    • Cmax: 0.111
    • Cmax_pos: 41
    • Smax: 0.156
    • Smax_pos: 23
    • Smean: 0.114
    • D: 0.118
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927856 NCBI
  • RefSeq: NP_375389 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGLVRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDGCIAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAFIPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITYGDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITHDILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]