PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2162 [new locus tag: SA_RS12410 ]
• pan locus tag^?: SAUPAN005890000
• symbol: SA2162
• pan gene symbol^?: iruO
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2162 [new locus tag: SA_RS12410 ]
• symbol: SA2162
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2428800..2429795
• length: 996
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125090 NCBI
• RefSeq: NP_375485 NCBI
• BioCyc: see SA_RS12410
• MicrobesOnline: 104511 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  TTGTATGCAAGCTTTTATGCTGGATTGCGAGATATGTCTGTAAGATTGATAGATGTTCAA
  TCTGAATTAGGGGGTAAGATGAGAATTTACCCTGAAAAAATTATTTGGGATATTGGCGGC
  ATTGCGCCAAAACCTTGTCATGAAATTTTAAAAGATACAATTAAGCAAGGATTATATTTT
  AAACCGGAAGTTCATTTGAATGAACGTGTGGTAGATATTAGGAAAAAGGCAGAACGTCAT
  TTCGAAGTTGAAACAGAAGCGGGAGAAATATATACATCGAAAGCAGTTATCATTGCAATT
  GGTGCGGGTATTATTAATCCAAAACAATTAGATGTTAAAGGTGTAGAGAGATATCAATTA
  ACTAATTTACATTATGTTGTACAAAGTTACAGACGTTTCAAAGATAAAGATGTATTAATA
  TCAGGTGGAGGAAATACAGCATTAGATTGGGCGCATGATATTGCCAAGATTGCTAAAAGC
  GTGACAGTTGTTTATCGAAAAGAAGATGTAAGTGGTCATGAAGCGATGAAAACGTTGGTG
  ACAGATTTAAATGTGAAACTATGCCCAAAAACACGTATTAAATACTTGGTCGGCAATGAT
  GATGAAACGCATATAAGTGAAGTTGTTTTGGAACATGTTGAAAGTGGCGATAGGCATACA
  GTTAAATTTGACGATGTCATTATTAGTCATGGGTTTGATCGTTGTAATACATTATTGAGT
  GAAACATCTTCAAAGCTAGATATGCATGATGATTGTCGTGTTAAAGGATTTGGTAATACG
  ACAACTAGTATACCTGGTATCTATGCGTGTGGAGATATTGTTTATCATGATGCAAAATCA
  CATCTAATTGCAAGTGCATTTAGTGATGGTGCAAATGCAGCGAACCTTGCTAAAACATAT
  ATCCAACCAGATGCGAATGCAGAGGGTTATGTTTCAAGTCATCATGAAGTGTTTAAAGAA
  GCGAATAAGACTATTGTAAATAAACATTTATACTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  996
  

⊟Protein

⊟General

• locus tag: SA2162 [new locus tag: SA_RS12410 ]
• symbol: SA2162
• description: hypothetical protein
• length: 331
• theoretical pI: 6.95906
• theoretical MW: 37033.9
• GRAVY: -0.305136

⊟Function

• reaction:
  EC 1.18.1.2?  ExPASy
  Ferredoxin--NADP⁺ reductase 2 reduced ferredoxin + NADP⁺ + H⁺ = 2 oxidized ferredoxin + NADPH
• TIGRFAM:
  Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 108.3)
  and 18 more
  Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 77.1)
  Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 77.1)
  Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 66.3)
  putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 66)
  Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 46)
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 42)
  mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 36.5)
  Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 35)
  Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 34.4)
  glutamate synthase, small subunit (TIGR01318; HMM-score: 28.9)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 28.3)
  putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 27.9)
  thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 26.7)
  glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 26.3)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 21)
  Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 19.7)
  flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 15.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.5)
• TheSEED  :
  • Thioredoxin reductase (EC 1.8.1.9)
  Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Thioredoxin reductase (EC 1.8.1.9)
  and 1 more
  Sulfur Metabolism Sulfur Metabolism - no subcategory Thioredoxin-disulfide reductase  Thioredoxin reductase (EC 1.8.1.9)
• PFAM:
  NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 85.6)
  Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 77.7)
  and 9 more
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 41.3)
  K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 29.9)
  Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 19.5)
  HI0933_like; HI0933-like protein (PF03486; HMM-score: 17)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 16.1)
  FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 14.7)
  NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 14.4)
  FAD_binding_2; FAD binding domain (PF00890; HMM-score: 12.8)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FAD
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005124
  • TAT(Tat/SPI): 0.000241
  • LIPO(Sec/SPII): 0.001734
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927952 NCBI
• RefSeq: NP_375485 NCBI
• UniProt: Q7A3W1 UniProt

⊟Protein sequence

• MYASFYAGLRDMSVRLIDVQSELGGKMRIYPEKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGEIYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTALDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHISEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTSIPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEANKTIVNKHLY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2161 < SA2162

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Slade A Loutet, Marek J Kobylarz, Crystal H T Chau, Michael E P Murphy
IruO is a reductase for heme degradation by IsdI and IsdG proteins in Staphylococcus aureus.
J Biol Chem: 2013, 288(36);25749-59
[PubMed:23893407] [WorldCat.org] [DOI] (I p)