NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2167 [new locus tag: SA_RS12445 ]
• pan locus tag^?: SAUPAN005897000
• symbol: scrA
• pan gene symbol^?: scrA
• synonym:
• product: PTS system sucrose-specific transporter subunit IIBC

⊟Gene

⊟General

• type: CDS
• locus tag: SA2167 [new locus tag: SA_RS12445 ]
• symbol: scrA
• product: PTS system sucrose-specific transporter subunit IIBC
• replicon: chromosome
• strand: -
• coordinates: 2433841..2435283
• length: 1443
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125095 NCBI
• RefSeq: NP_375490 NCBI
• MicrobesOnline: 104516 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGAATTATAAGCAATCCGCAGAAGATATTTTGAACGCGATAGGCGGAGAAGAGAATTTA
  GATGCAATGGCGCATTGTGCAACGAGACTACGATTAGTTTTAAATGATGAAAGTTTAGTA
  AATGAAGAGGCGCTAAACAATATGGATGTAGTTAAAGGGACGTTTTCTACTGGGGGACAA
  TACCAAATTATTATTGGGTCTGGTACAGTCAATAAAGTATTTAGTGAACTGGAAAAATTA
  ACTGGAAAAGAAGCATCAACCACTTCGGAAGTCAAAGCACAATCTGCTAAAAATATGAAT
  CCGTTACAGCGATTTGTAAAAATGCTTTCAGATATCTTTGTTCCGATTATACCAGCCATC
  GTTGCTGGTGGTTTATTAATGGGGTTAAATAACATTTTGACTGCGAAAGATTTATTCTTT
  TCAGGTAAATCATTGATAGATGTATATAGTCAATTTGCTGGATTAGCTGAAATGATAAAT
  GTTTTTGCGAATGCACCATTTACATTATTACCAATTTTAATTGGATTTAGTGCAGCAAAA
  CGCTTTGGTGGCAATCCATTTTTAGGTGCTGTATTAGGTATGATACTAGTTCATCCATCG
  CTAATGAGCGCATACGATTTCCCAAAAGCAGTTGAAGCAGGTAAGGCTATTCCATATTGG
  GATGTTTTTGGTTTGCATATTAATCAAGTAGGTTATCAAGGACAAGTGTTACCTATGCTT
  GTAGCAGCTTATATCTTAGCCTCAATTGAAAAAGGGTTACGCAAAGTTATTCCAACGGTG
  TTAGATAATTTGTTAACACCATTGTTATCTATTTTTATAACAGCATTTCTAACATTTTCA
  TTTGTAGGTCCAATCACTCGACAATTAGGTTACTGGTTATCAGATGGTTTAACATGGCTT
  TATGAATTTGGTGGTGCAATTGGTGGATTAATATTCGGATTATTGTATGCTCCGATTGTT
  ATTACAGGTATGCATCATAGCTTTATAGCTGTAGAAACGACATTAATTGCAGATGCCACT
  AAAACGGGTGGATCATTTATATTCCCGATTGCGACAATGTCTAATGTTGCACAAGGTGGT
  GCAGCAATTGCAGCGTTCTTTATTATTAAACAAAATAAGAAGTTAAAAGGTGTGGCATCT
  GCCGCAGGTATTTCAGCATTACTTGGTATTACAGAACCGGCTATGTTTGGTGTTAACTTA
  AAACTAAGATATCCATTTATTGGCGCTATCGTTGGATCAGGTATTGGTTCAGCATATATT
  GCTTTCTTCAAGGTTAAAGCAATCGCATTAGGAACTGCTGGATTGCCAGGATTTATTTCA
  ATCAATCCAGTACATGCAGGATGGTTACACTACTTTGTTGGTATGACAATATCATTCATC
  ATTGCTATAACAGTTACTTTAATTTTATCTAAAAGAAAAGCAAATAAAGAAGTTGTAGAA
  TAA
  60
  120
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  1140
  1200
  1260
  1320
  1380
  1440
  1443
  

⊟Protein

⊟General

• locus tag: SA2167 [new locus tag: SA_RS12445 ]
• symbol: ScrA
• description: PTS system sucrose-specific transporter subunit IIBC
• length: 480
• theoretical pI: 9.25364
• theoretical MW: 51231.9
• GRAVY: 0.584583

⊟Function

• reaction:
• TIGRFAM:
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 676.4)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 555.5)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 368.4)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 273)
  Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 273)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 85.8)
  Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 85.8)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 42.2)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 38.1)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 35.8)
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 29.2)
  glycopeptide, sublancin family (TIGR04196; HMM-score: 13.8)
• TheSEED:  
  Sucrose utilization PTS system, sucrose-specific IIB component (EC 2.7.1.69) / PTS system, sucrose-specific IIC component 
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 204.6)
  no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 50.9)
  GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 12.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.13
  • Ymax_pos: 45
  • Cmax: 0.126
  • Cmax_pos: 25
  • Smax: 0.242
  • Smax_pos: 40
  • Smean: 0.096
  • D: 0.117
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 15927957 NCBI
• RefSeq: NP_375490 NCBI
• UniProt: A0A0H3JRI8 UniProt

⊟Protein sequence

• MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEALNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPLQRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMINVFANAPFTLLPILIGFSAAKRFGGNPFLGAVLGMILVHPSLMSAYDFPKAVEAGKAIPYWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAFLTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETTLIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITEPAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLHYFVGMTISFIIAITVTLILSKRKANKEVVE

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• scrA no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulators: CcpA regulon, ScrR regulon

  CcpA	(TF)	important in Carbon catabolism	RegPrecise
  ScrR	(TF)	important in Sucrose utilization	RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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