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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2248 [new locus tag: SA_RS12915 ]
  • pan locus tag?: SAUPAN006018000
  • symbol: SA2248
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2248 [new locus tag: SA_RS12915 ]
  • symbol: SA2248
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2523999..2525576
  • length: 1578
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACGTTTCTTACAGTCATGCAATTTATAGTTAACATTATCGTTGTAGGCTTCCTGCTT
    ACGGTTATTGTTATCGGGCTTATTTGGTTAATTAAAGATAAAAGACAATCACAACATAGT
    GTATTAAGGAATTATCCTTTACTAGCACGTATTAGATATATTTCAGAAAAAATGGGACCG
    GAATTACGTCAGTATTTATTTTCTGGGGATAATGAAGGGAAACCTTTTTCACGTAATGAT
    TATAAAAATATCGTTTTGGCTGGAAAATATAACTCTCGTATGACCAGCTTCGGTACTACT
    AAAGATTATCAAGACGGCTTTTACATACAGAACACAATGTTTCCGATGCAACGTAATGAG
    ATTTCAGTAGATAATACAACATTGTTATCAACATTCATTTATAAAATCGCGAATGAGCGT
    TTATTTAGTCGTGAAGAATATCGTGTACCGACAAAGATTGATCCGTATTACTTAAGTGAT
    GACCATGCAATAAAATTAGGTGAACATTTAAAACATCCATTTATTTTAAAACGTATCGTA
    GGACAATCTGGTATGAGTTATGGCGCTTTAGGAAAAAATGCCATTACAGCTTTATCTAAA
    GGTCTAGCTAAAGCGGGCACTTGGATGAATACAGGTGAAGGTGGCTTATCAGAATATCAT
    TTAAAAGGTAATGGGGATATCATTTTCCAAATTGGTCCCGGTTTATTTGGTGTTCGTGAT
    AAAGAAGGTAATTTTAGTGAAGGTTTATTTAAAGAGGTCGCACAGTTATCTAACGTACGC
    GCATTTGAGCTGAAGTTGGCACAAGGTGCTAAGACTCGTGGTGGTCATATGGAAGCTGAA
    AAGGTAAACGAAGAAATTGCTAAAATCCGAAATGTTGAACCTTATAAAACAATCAATTCA
    CCTAACCGTTACGAATTTATTCATAATGCTGAAGATTTGATTCGTTTCGTCGATCAGTTG
    CAGCAATTAGGTCAAAAACCAGTAGGATTCAAAATTGTAGTAAGCAAAGTTTCAGAAATT
    GAAACACTTGTACGTACGATGGTGGAACTAGATAAGTATCCAAGCTTTATTACGATTGAT
    GGTGGTGAAGGTGGTACTGGTGCAACATTCCAAGAATTACAAGATGGTGTTGGCTTACCG
    CTATTTACAGCTCTACCTATTGTGTCTGGCATGTTAGAAAAATATGGTATTCGAGATAAA
    GTGAAATTGGCGGCATCTGGTAAGTTAGTGACACCAGATAAAATTGCGATTGCACTAGGT
    TTAGGTGCAGATTTTGTAAATATCGCACGTGGCATGATGATTAGTGTCGGTTGTATTATG
    AGTCAACAATGCCATATGAATACATGTCCTGTAGGTGTTGCAACGACAGATGCGAAAAAA
    GAAAAAGCATTGATTGTTGGAGAAAAGCAATATCGTGTCACAAACTATGTAACAAGTTTG
    CATGAAGGCTTATTCAATATTGCAGCAGCTGTTGGCGTATCCAGTCCTACAGAAATTACT
    GCTGATCATATTGTATATCGAAAAGTCGATGGTGAGTTACAAACGATACATGATTATAAA
    TTGAAATTGATTAGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2248 [new locus tag: SA_RS12915 ]
  • symbol: SA2248
  • description: hypothetical protein
  • length: 525
  • theoretical pI: 9.24714
  • theoretical MW: 58421.1
  • GRAVY: -0.119238

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    PfaD family protein (TIGR02814; HMM-score: 12.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherisopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 10.9)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis Ferredoxin-dependent glutamate synthase (EC 1.4.7.1) 
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryAmmonia assimilation Ferredoxin-dependent glutamate synthase (EC 1.4.7.1) 
  • PFAM:
    TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 332.5)
    Glu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 18.7)
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 15.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 38
    • Cmax: 0.18
    • Cmax_pos: 38
    • Smax: 0.251
    • Smax_pos: 6
    • Smean: 0.115
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRNYPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKDYQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSDDHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSEYHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGHMEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVVSKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGMLEKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCPVGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRKVDGELQTIHDYKLKLIS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA2248 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]