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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2499 [new locus tag: SA_RS14290 ]
  • pan locus tag?: SAUPAN006487000
  • symbol: gidB
  • pan gene symbol?: gidB
  • synonym:
  • product: 16S rRNA methyltransferase GidB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2499 [new locus tag: SA_RS14290 ]
  • symbol: gidB
  • product: 16S rRNA methyltransferase GidB
  • replicon: chromosome
  • strand: -
  • coordinates: 2809792..2810511
  • length: 720
  • essential: yes DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACTGTAGAATGGTTAGCAGAACAATTAAAAGAACATAATATTGAATTAACTGAGACT
    CAAAAACAACAGTTTCAAACATATTATCGTTTACTTGTTGAATGGAATGAAAAGATGAAT
    TTGACAAGTATTACAGATGAACACGATGTATATTTGAAACATTTTTATGATTCCATTGCA
    CCTAGTTTTTATTTTGATTTTAATCAGCCTATAAGTATATGTGATGTAGGCGCTGGAGCT
    GGTTTTCCAAGTATTCCGTTAAAAATAATGTTTCCGCAGTTAAAAGTGACGATTGTTGAT
    TCATTAAATAAGCGTATTCAATTTTTAAACCATTTAGCGTCAGAATTACAATTACAGGAT
    GTCAGCTTTATACACGATAGAGCAGAAACATTTGGTAAGGGTGTCTACAGGGAGTCTTAT
    GATGTTGTTACTGCAAGAGCAGTAGCTAGATTATCCGTGTTGAGTGAATTGTGTTTACCG
    CTAATTAAAAAAGGTGGACAGTTTGTTGCATTAAAATCTTCAAAAGGTGAAGAAGAATTA
    GAAGAAGCAAAATTTGCAATTAGTGTGTTAGGTGGTAACGTTACAGAAACACATACCTTT
    AAATTGCCAGAAGATGCTGGAGAGCGCCAGATGTTCATTATTGATAAAAAAAGACAGACG
    CCGAAAAAGTACCCAAGAAAACCAGGGACGCCTAATAAGACTCCTTTACTTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2499 [new locus tag: SA_RS14290 ]
  • symbol: GidB
  • description: 16S rRNA methyltransferase GidB
  • length: 239
  • theoretical pI: 6.79958
  • theoretical MW: 27373.2
  • GRAVY: -0.371548

Function[edit | edit source]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 173.8)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 28.9)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 22.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 20.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 20)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 17.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll C-20 methyltransferase BchU (TIGR02716; EC 2.1.1.-; HMM-score: 16)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 14)
  • TheSEED  :
    • 16S rRNA (guanine(527)-N(7))-methyltransferase (EC 2.1.1.170)
    Cell Division Subsystem including YidCD  rRNA small subunit methyltransferase, glucose inhibited division protein GidB
  • PFAM:
    NADP_Rossmann (CL0063) GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 195.9)
    and 6 more
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 33.1)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 24.8)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 17.7)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 16.3)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 15.9)
    TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 11.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001883
    • TAT(Tat/SPI): 0.000185
    • LIPO(Sec/SPII): 0.000514
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPLIKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]