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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0089 [new locus tag: SACOL_RS00445 ]
  • pan locus tag?: SAUPAN000902000
  • symbol: SACOL0089
  • pan gene symbol?:
  • synonym:
  • product: myosin-cross-reactive antigen

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0089 [new locus tag: SACOL_RS00445 ]
  • symbol: SACOL0089
  • product: myosin-cross-reactive antigen
  • replicon: chromosome
  • strand: -
  • coordinates: 100187..101962
  • length: 1776
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTATTACAGTTATGGAAATTATGAAGCATTTGCGCGCCCTAAAAAACCTGAAAATGTA
    GAAAACAAATCCGCTTACTTAATCGGATCTGGTCTAGCTTCACTTGCTGCAGCTTGTTTT
    TTAATAAGGGATGGTCAAATGGAAGGTTCGAAGATTCATATTTTAGAAGAGTTACCTAAA
    GCAGGTGGTAGTCTTGATGGTGAAAATATGCCTTTAAAAGGCTATGTTGTCCGCGGTGGT
    CGTGAAATGGAGAACCACTTTGAATGTTTGTGGGACTTATTCAGATCTATCCCTTCATTA
    GAAATCGATAACGCGTCTGTATTAGATGAGTTCTATTGGCTAAACAAAGAAGACCCTAAC
    TATTCTCGCTGTCGTGTTATTGAGAAACAGGGTCAACGTTTAGTCACAGACGGAGACTTC
    ACTTTGACTAAAACGGCGATTAAAGAAATTTTAGATTTATGCTTAACGAATGAAGAAGAT
    TTAGATGATGTCAAAATAACAGATGTATTTTCCGATGACTTCTTTAATTCAAACTTTTGG
    ATTTACTGGAAAACGATGTTTGCATTTGAACCGTGGCATTCTGCAATGGAAATGCGTCGC
    TATCTAATGCGATTCGTTCATCATATTAGTGGTCTCGCAGACTTTTCAGCTTTAAAATTC
    ACTAAATATAATCAATATGAATCTTTAGTATTACCTATGGTTGAATATTTAAAATCGCAT
    GGGGTTCAATTTGAATACGATGTAAAAGTCGAAGATATTAAAATAGATGTTACGACAAGT
    CAAAAAATTGCCCGAGAAATATTAATTGACCGTAATGGTAATGCAGAATCTATTAAACTG
    ACTATAAACGATCTTGTCTTTGTGACAAACGGTAGTATTACAGAAAGCTCTACTTATGGT
    GATAATGATACACCAGCGCCACCAACTGACGAATTAGGTGGTAGTTGGACACTATGGAAA
    AATTTAGCGCGACAAAGTCCTGAATTTGGTAATCCTGATAAGTTTTGCCAAAATATTCCT
    AAAAAAAGTTGGTTTGTTTCAGCAACTTCTACAACAAACAATAAAGAGATTATCGATACA
    ATAGAAAGTATTTGTAAACGTGACCCACTTGCAGGCAAAACAGTTACAGGCGGTATTATT
    ACAATCAATGATTCTGCATGGCAAATGAGTTTTACAATCAATCGTCAGCAACAGTTTAAA
    GACCAACCTGAAAATGAAATATCTACATGGATTTATGCCTTATATTCAGATGTAAACGGC
    GATTATATTAAAAAGCCAATTACAGAATGTAGTGGTAATGAAATATGCCAAGAATGGCTG
    TATCACTTAGGTGTATCAACTGACAAAATTGAAGACTTAGCAAAACATGCATCTAATACG
    ATTCCTGTTTATATGCCATATATCACATCTTATTTCATGACGCGTGCTATCGGCGACAGA
    CCTTTAGTCGTCCCGCATCAATCTCAGAACTTAGCATTTATTGGTAACTTTGCAGAAACA
    GAGCGAGACACTGTATTTACAACAGAATATTCGGTTCGTACTGCCATGGAAGCTGTTTAT
    CAATTACTAAATATAGATCGTGGTATTCCAGAAGTCATCAATAGTCCATTTGATCTTCGC
    GTCTTAATGGATGCCATATACGAACTGAATGACCACCAAGATTTGCGTGAGATTACTAAA
    GATTCGAAAATGCAAAAACTCGCATTAGCAGGATTCCTTAAAAAGATAAAAGGTACGTAC
    ATTGAGTCATTATTAAAAGAACACAAATTGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1776

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0089 [new locus tag: SACOL_RS00445 ]
  • symbol: SACOL0089
  • description: myosin-cross-reactive antigen
  • length: 591
  • theoretical pI: 4.75677
  • theoretical MW: 67648.2
  • GRAVY: -0.371235

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15.7)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 14.8)
  • TheSEED:  
    Oleate hydratase (EC 4.2.1.53) 
  • PFAM:
    NADP_Rossmann (CL0063) MCRA; MCRA family (PF06100; HMM-score: 746.4)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25)
    P-loop_NTPase (CL0023) AAA_15; AAA ATPase domain (PF13175; HMM-score: 15)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 39
    • Cmax: 0.122
    • Cmax_pos: 39
    • Smax: 0.232
    • Smax_pos: 42
    • Smean: 0.127
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRDGQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAESIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDKFCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFTINRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKIEDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQKLALAGFLKKIKGTYIESLLKEHKLL

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: RpoF (SigB*) [2] other strains
    RpoF  (SigB*) (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]