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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0169 [new locus tag: SACOL_RS00860 ]
  • pan locus tag?: SAUPAN001024000
  • symbol: argC
  • pan gene symbol?: argC
  • synonym:
  • product: N-acetyl-gamma-glutamyl-phosphate reductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0169 [new locus tag: SACOL_RS00860 ]
  • symbol: argC
  • product: N-acetyl-gamma-glutamyl-phosphate reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 193900..194931
  • length: 1032
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTAAAGTAGGTATCGTTGGCGGTAGCGGTTATGGCGCAATTGAATTAATTCGATTG
    TTACAAACACATCCTCATGTAACGATTGCACACATCTACTCACATTCAAAAGTAGATGAA
    CCGTTGAAATTAACATTTCCACATTTACAACATATTATGCAACATTTCGAAGCACTTACA
    GTGGACAATAATGACTGTGATGTAATTTTCTTTGCGACACCAGCACCCGTAAGTAAAACA
    TGTATCCCTCCCTTAGTAGAAAAAGGTATTCATGTTATCGATTTATCTGGCGCATTTAGA
    ATTAAGAATCGTGAAATATATGAAGCATATTACAAAGAAACTGCTGCAGCACAAGATGAT
    TTGAATCATGCTATTTACAGCATTTCAGAATGGCAATCGTTTGATAACAATGGAACGAAG
    CTCATTTCTAATCCTGGCTGTTTCCCTACAGCAACATTATTAGCATTACATCCACTTATT
    AGCGAAAAAATAGTAGATTTGTCATCTATTATTATTGATGCTAAGACCGGCGTGTCAGGT
    GCTGGTCGTTCATTATCACAACGTGTTCATTTTTCAGAAATGAATGAAAATCTAAGCGCT
    TATGCAATCGGAAACCATAAACACAAACCGGAAATCGAGCAATATTTATCTATCATTGCG
    GGTCAAGATGTATCAGTCATATTTACACCACATCTCGTACCAATGACACGAGGTATTTTA
    TCAACAATATATGTCAAATTATCATCTGAATATACGACTGAATCATTACATAAATTAATG
    ACCTCTTATTATGCTAATCAGCCATTTGTCAGAATTAGAGATATTGGGACTTTTCCAACC
    ACAAAAGAAGTACTCGGTAGTAACTACTGCGATATCGGCATCTATGTAGATGAAACAACG
    CAAACAGCAATTTTAGTATCAGTGATTGATAACCTTGTCAAAGGCGCAAGTGGGCAAGCC
    ATTCAAAATTTAAATATATTATATGATTTTGAAGTGACGACTGGCCTAAATCAATCACCA
    GTTTATCCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1032

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0169 [new locus tag: SACOL_RS00860 ]
  • symbol: ArgC
  • description: N-acetyl-gamma-glutamyl-phosphate reductase
  • length: 343
  • theoretical pI: 6.22283
  • theoretical MW: 37890
  • GRAVY: 0.0157434

Function[edit source | edit]

  • reaction:
    EC 1.2.1.38?  ExPASy
    N-acetyl-gamma-glutamyl-phosphate reductaseN-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyN-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 440.3)
    MetabolismAmino acid biosynthesisGlutamate familyN-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 99.6)
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 59.4)
    MetabolismAmino acid biosynthesisAspartate familyaspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 45.7)
    acetaldehyde dehydrogenase (acetylating) (TIGR03215; EC 1.2.1.10; HMM-score: 13)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine Biosynthesis extended N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) 
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 99.6)
    NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 91.6)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 15.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 31
    • Cmax: 0.134
    • Cmax_pos: 33
    • Smax: 0.177
    • Smax_pos: 27
    • Smean: 0.12
    • D: 0.13
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKLTFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIKNREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLISEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSIIAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIGTFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTTGLNQSPVYP

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: ArgR
    ArgR (TF) important in Arginine biosynthesis, Arginine degradation

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]