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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0368 [new locus tag: SACOL_RS01850 ]
  • pan locus tag?: SAUPAN001833000
  • symbol: SACOL0368
  • pan gene symbol?:
  • synonym:
  • product: prophage L54a, HK97 family portal protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0368 [new locus tag: SACOL_RS01850 ]
  • symbol: SACOL0368
  • product: prophage L54a, HK97 family portal protein
  • replicon: chromosome
  • strand: +
  • coordinates: 375923..377161
  • length: 1239
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    GTGAATGTTATCGCAAAAGAGAATATTGTCACACGCATAAAGAAAAAATTGATAGACAAT
    TGGATTGATCAGTCAACTTCTAAGCTTTATGACTTTAGCCCATGGAAAAATAGATCTTTT
    TGGGGTGTAATTAATAATACGCTTGAAACTAATGAAACGATATTTTCAGCTATTACAAAG
    TTATCTAATTCGATGGCTAGTTTGCCCTTGAAAATGTATGAAGATTATAAAGTAGTTAAT
    ACAGAAGTATCTGATTTACTTACAGTGTCACCGAATAATTCTCTGAGCAGTTTTGATTTT
    ATTAATCAAATTGAAACAATCAGAAATGAAAAAGGTAATGCATATGTGCTAATTGAACGA
    GACATCTATCATCAACCATCAAAGCTTTTCTTATTAAATCCAGATGTTGTTGAAATGTTA
    ATTGAAAACCAATCACGTGAACTTTATTATTCCATTCATGCTGCAACTGGAAATAAATTG
    ATTGTTCATAATATGGACATGTTGCATTTTAAACACATCGTGGCATCTAATATGGTGCAA
    GGCATTAGTCCGATTGATGTGTTGAAGAATACAACTGATTTTGATAATGCAGTAAGAACC
    TTTAATCTTACAGAAATGCAAAAACCTGATTCTTTCATGCTTAAATATGGTTCCAATGTA
    GGTAAAGAAAAAAGGCAGCAAGTGTTAGAAGATTTCAAACAGTACTATGAAGAAAACGGT
    GGAATATTATTCCAAGAGCCTGGTGTTGAAATCGAACCGTTACCTAAAAAATATGTCTCT
    GAAGATATAGTGGCAAGCGAGAATTTAACAAGAGAAAGAGTAGCTAACGTTTTTCAATTG
    CCCTCAGTATTCTTAAATGCAAGATCAAATACAAATTTCGCGAAAAATGAAGAGTTAAAC
    AGATTTTACTTGCAGCATACCTTATTGCCAATCGTCAAACAGTATGAAGAAGAATTTAAT
    CGGAAACTACTTACTAAAACAGACAGAGAAAAAAATAGGTATTTTAAATTTAACGTTAAA
    TCTTATTTAAGGGCTGATAGTGCAACACAAGCAGAAGTGTACTTTAAAGCAGTTCGTAGT
    GGTTACTACACTATAAATGACATTAGAGAGTGGGAGGATTTACCACCAGTTGAAGGTGGA
    GATAAGCCGCTAATAAGCGGTGATTTATACCCAATTGACACGCCACTTGAATTAAGAAAA
    TCTTTGAAAGGTGGTGATAAAAATGTCAATGAAAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0368 [new locus tag: SACOL_RS01850 ]
  • symbol: SACOL0368
  • description: prophage L54a, HK97 family portal protein
  • length: 412
  • theoretical pI: 5.78936
  • theoretical MW: 47727.8
  • GRAVY: -0.520388

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage portal protein, HK97 family (TIGR01537; HMM-score: 342.1)
  • TheSEED:  
    Phage portal protein 
  • PFAM:
    no clan definedPhage_portal; Phage portal protein (PF04860; HMM-score: 228.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 11
    • Cmax: 0.142
    • Cmax_pos: 58
    • Smax: 0.391
    • Smax_pos: 2
    • Smean: 0.257
    • D: 0.198
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNVIAKENIVTRIKKKLIDNWIDQSTSKLYDFSPWKNRSFWGVINNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFINQIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKLIVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGSNVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVANVFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRYFKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPIDTPLELRKSLKGGDKNVNES

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]