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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0409 [new locus tag: SACOL_RS02060 ]
  • pan locus tag?: SAUPAN001875000
  • symbol: SACOL0409
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0409 [new locus tag: SACOL_RS02060 ]
  • symbol: SACOL0409
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 415962..417023
  • length: 1062
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCAAATTAGAAATGAATAAAAATACGCCTCTTGAGTTTGGTTTGTATTCCTTAGGT
    GATCATTTATTGAATCCATTGAAAGGTGAAAAAGTTAGTTATGAGCAACGTATTAATGAA
    ATTATTGAAGCAAGTAAATTAGCAGATGAAGCAGGTATTGATGTTTTTGCAGTTGGTGAA
    AGTCATCAGGAGCATTTTACAACACAGGCACATACGGTTGTGTTAGGTGCAATTGCCCAA
    GCGACAAAGCATATTAAAGTTTCAAGTTCTTCAACGATTATTAGTGCAACAGATCCTGTA
    AGAGTATTTGAAGACTTCGCGACATTAGATTTGATTTCTCATGGTAGAGCCGAAATTGTA
    GCTGGCAGAGCATCAAGAACAGGTATTTTTGACTTGTTTGGCTATGATTTAAAAGACTAT
    GATGAATTGTTTGAAGAAAAATTAGGTTTACTTTTAGAGTTAAATAAAACTGAGCGTATT
    ACTTGGTCTGGAAAATATCGTCCAGAACTTAGAAATATGAAAATATTCCCAAGACCAATC
    GATAATATATTGCCAATATGGCGTGCTGTTGGTGGTCCACCTGCAAGTGCTATTAAAGCG
    GGAAAACAAGGTGTGCCAATGATGATTACAACCCTTGGTGGCCCAGCAATGAACTTTAAA
    GGTTCTATAGATGCTTATCGTCAAGCGGCAACTGAAGCAGGTTTCGATGCTTCGCCTAAG
    TCTTTACCAGTAAGTACAGCGAGTCTGTTTTATACAGCTGAAACAACTCAGGATGCTATG
    AGAGAATTTTATCCACATTTGAATACAGGGATGTCATTTATTCGTGGTGTTGGTTATCCG
    AAACAGCAATTTGCTAATTCGTCAGATTATCGAGAAGCGCTAATGGTTGGAAGCCCGCAA
    CAAATTATTGAAAAGATATTGTATCAACACGAGTTGTATGGTCATCAACGTTTTATGGCA
    CAGCTTGATTTTGGCGGTGTGCCATTTGAAAATGTTATGAAGAATATTGAGTTAATTGGC
    AACGACATTATACCGGCGATTAAAAAGCATTTATCAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0409 [new locus tag: SACOL_RS02060 ]
  • symbol: SACOL0409
  • description: hypothetical protein
  • length: 353
  • theoretical pI: 6.52142
  • theoretical MW: 39171.5
  • GRAVY: -0.220397

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    probable oxidoreductase, LLM family (TIGR03858; EC 1.-.-.-; HMM-score: 348.7)
    Unknown functionEnzymes of unknown specificityputative luciferase-like monooxygenase, FMN-dependent, CE1758 family (TIGR04036; EC 1.-.-.-; HMM-score: 213.8)
    natural product biosynthesis luciferase-like monooxygenase domain (TIGR04020; EC 1.-.-.-; HMM-score: 91.7)
    Unknown functionEnzymes of unknown specificityluciferase family oxidoreductase, group 1 (TIGR03558; EC 1.-.-.-; HMM-score: 62.4)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv2161c family (TIGR03619; EC 1.-.-.-; HMM-score: 58.5)
    Unknown functionEnzymes of unknown specificityputative FMN-dependent luciferase-like monooxygenase, KPN_01858 family (TIGR04027; EC 1.-.-.-; HMM-score: 52.9)
    Unknown functionEnzymes of unknown specificityluciferase-type oxidoreductase, BA3436 family (TIGR03571; EC 1.-.-.-; HMM-score: 44.8)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 40.5)
    MetabolismEnergy metabolismMethanogenesis5,10-methylenetetrahydromethanopterin reductase (TIGR03555; EC 1.5.98.2; HMM-score: 37.9)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_2516 family (TIGR03621; HMM-score: 35.8)
    Unknown functionEnzymes of unknown specificityF420-dependent oxidoreductase, G6PDH family (TIGR03557; EC 1.-.-.-; HMM-score: 29.3)
    MetabolismCentral intermediary metabolismSulfur metabolismdimethyl sulfone monooxygenase SfnG (TIGR04021; EC 1.14.-.-; HMM-score: 28.5)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_2249 family (TIGR03857; HMM-score: 28.4)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_3544 family (TIGR03854; HMM-score: 28)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv3520c family (TIGR03559; HMM-score: 26.9)
    MetabolismEnergy metabolismPentose phosphate pathwayglucose-6-phosphate dehydrogenase (coenzyme-F420) (TIGR03554; EC 1.1.98.2; HMM-score: 26.7)
    Unknown functionEnzymes of unknown specificityFMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family (TIGR03860; EC 1.-.-.-; HMM-score: 23.5)
    Unknown functionEnzymes of unknown specificityF420-dependent oxidoreductase, CPS_4043 family (TIGR03842; HMM-score: 20.5)
    Unknown functionEnzymes of unknown specificitycoenzyme F420-dependent oxidoreductase, NP1902A family (TIGR04024; EC 1.14.-.-; HMM-score: 17.7)
    Unknown functionEnzymes of unknown specificityF420-dependent oxidoreductase, MSMEG_4879 family (TIGR03564; EC 1.-.-.-; HMM-score: 15.9)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_2906 family (TIGR03856; HMM-score: 11.8)
  • TheSEED:  
    Bacterial luciferase family protein 
  • PFAM:
    no clan definedBac_luciferase; Luciferase-like monooxygenase (PF00296; HMM-score: 142.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 12
    • Cmax: 0.112
    • Cmax_pos: 30
    • Smax: 0.189
    • Smax_pos: 29
    • Smean: 0.145
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEASKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRVFEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERITWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMNFKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRGVGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMKNIELIGNDIIPAIKKHLSK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: RpoF (SigB*) [4] other strains
    RpoF  (SigB*) (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]