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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0468 [new locus tag: SACOL_RS02370 ]
  • pan locus tag?: SAUPAN002104000
  • symbol: SACOL0468
  • pan gene symbol?: ssl1
  • synonym:
  • product: superantigen-like protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0468 [new locus tag: SACOL_RS02370 ]
  • symbol: SACOL0468
  • product: superantigen-like protein
  • replicon: chromosome
  • strand: +
  • coordinates: 470357..471037
  • length: 681
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAATTTAAAGCGATAGCAAAAGCAAGTTTAGCATTGGGAATGTTAGCAACAGGTGTA
    ATTACATCGAATGTACAATCAGTACAAGCGAAAGCAGAAGTTAAACAACAAAGTGAATCA
    GAGTTAAAACACTATTATAATAAACCAATTTTAGAGCGTAAAAATGTGACTGGATTTAAA
    TATACTGATGAGGGTAAACACTATTTAGAAGTCACAGTAGGGCAACAGCATTCTCGAATC
    ACTTTACTTGGATCTGATAAAGATAAATTTAAAGACGGAGAAAACTCAAATATAGATGTG
    TTTATCCTTAGAGAAGGTGACAGTAGACAAGCAACAAATTACTCAATTGGTGGCGTTACA
    AAATCAAATAGTGTGCAGTATATTGATTATATCAATACGCCAATTTTAGAAATCAAGAAA
    GATAATGAAGATGTACTTAAAGATTTTTACTACATTTCAAAAGAAGACATCTCATTAAAA
    GAACTTGATTATAGATTAAGAGAACGTGCGATTAAACAACACGGCTTGTATTCAAATGGT
    CTTAAACAAGGTCAAATTACAATTACAATGAATGATGGCACAACACATACAATCGATTTA
    AGTCAAAAACTTGAAAAAGAACGTATGGGTGAGTCAATCGACGGCACTAAGATTAATAAA
    ATTCTAGTAGAAATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    681

Protein[edit source | edit]

Protein Data Bank: 4RH6
Protein Data Bank: 4O1N

General[edit source | edit]

  • locus tag: SACOL0468 [new locus tag: SACOL_RS02370 ]
  • symbol: SACOL0468
  • description: superantigen-like protein
  • length: 226
  • theoretical pI: 9.02582
  • theoretical MW: 25646.9
  • GRAVY: -0.642035

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Exotoxin 6 
  • PFAM:
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 110.1)
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 21.6)
    MAP; MAP domain (PF03642; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.16
    • Extracellular Score: 9.7
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 3
    • Predicted Cleavage Site: SVQAKAEV
  • SignalP: Signal peptide length 31 aa
    • Ymax: 0.689
    • Ymax_pos: 31
    • Cmax: 0.571
    • Cmax_pos: 31
    • Smax: 0.902
    • Smax_pos: 13
    • Smean: 0.851
    • D: 0.752
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKHYYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFILREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLKELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKINKILVEMK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol. Med. Microbiol.: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)