From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0494 [new locus tag: SACOL_RS02495 ]
  • pan locus tag?: SAUPAN002163000
  • symbol: nuoF
  • pan gene symbol?: mpsA
  • synonym:
  • product: NADH dehydrogenase subunit 5

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0494 [new locus tag: SACOL_RS02495 ]
  • symbol: nuoF
  • product: NADH dehydrogenase subunit 5
  • replicon: chromosome
  • strand: +
  • coordinates: 493838..495322
  • length: 1485
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    GTGTTAAGTTTTCAATTGCTATTTTCACTGTTTGTTATTGCGCTTATCATTGCATTGATA
    AGTGGCTTGTTGTTTTTAGCACCAGTTATGCCAATGAGATATATTAAATTACATTTATAC
    ATACTAGTCATGCCAGTATTATTTGCAGTCATTGGCTTTTTCGGTATTCATGGTCAACAT
    GTCTTAGGTCCATTTAAAATAGATCGTTTATCTTGGTTATTAGCTGGCTTTGTAATGGCG
    CTTGGCTTTATTATTCAAAAGTTTTCAATGCGATATTTACTAGGTGATCATCATTATAGA
    CATTACTTTCCATTGTTCACTGCGATTACGTCGTTTGCATCTTTAGCATGGATGTCTGAA
    GACTTAAGACTGATGGCACTCTGCTGGGGTATTACATTATTATGTTTAACATTGCTGATG
    AACGTTAATCGTTTTTGGAAAGTGCCACGTGAGTCTGCGAAATTATCAAGCATGACATTT
    TTATGTGGTTGGCTTGCATTCGTTGGAGCAATTGTAACTATTTATATTGCGACTGGCGAG
    TGGCGGGTACCACAACATATAGTTCATCCGACATGGTCATTGTTGACGAATGTACTACTT
    GTATTAGCTGTCATGATACCGGCAGCACAATTTCCTTTTCATCGATGGTTGATTGAATCT
    GTAACGGCACCAACGCCAGTATCGGCAATTATGCATGCAGGAATTGTGAATGCAGGTGGT
    GTTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTT
    ATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGGTATTAGCTTAGTTCAAGTTGACTAT
    AAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCA
    TTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACA
    TTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT
    AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTA
    TTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGG
    TCATTACTTGTATCTTGGAATCAAATGGTAGCCTTTAGTAAAGGACGCATGGCACGTCTT
    GTTGGTATGATTTTGATTGCAATCGTGACATTTATCTACGTCATCACACATAATTATTTT
    TACGCTGTATTACAAAATATAACAACACATGCGACAACGCCGCCTACAGTGAGTGTCATC
    ATTAGTGTTGTTATTTTAATCTTTGGCAGTTTATTAAGTATTTGGGTGGCGCGGCATCGA
    TACTCTAAGGCTTTTGAGGTATTGTACGTGTGGTTAGTTAATCTAGGTGAAGCACGCTCG
    AAAGCGATAGAAAGTCATCCGAATTATTTGAAGAAGTATTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0494 [new locus tag: SACOL_RS02495 ]
  • symbol: NuoF
  • description: NADH dehydrogenase subunit 5
  • length: 494
  • theoretical pI: 10.2961
  • theoretical MW: 55314.2
  • GRAVY: 0.90668

Function[edit source | edit]

  • reaction:
    EC 1.6.5.3?  ExPASy
    NADH:ubiquinone reductase (H+-translocating)NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out)
  • TIGRFAM:
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 128.6)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmonovalent cation:proton antiporter (TIGR00940; HMM-score: 88.7)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 56.3)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 47.3)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 27.2)
  • TheSEED:  
    NADH dehydrogenase, subunit 5 
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 175.5)
    no clan definedProton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 22.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.14
    • Ymax_pos: 11
    • Cmax: 0.147
    • Cmax_pos: 34
    • Smax: 0.273
    • Smax_pos: 3
    • Smean: 0.197
    • D: 0.162
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTFLCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAFSKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGSLLSIWVARHRYSKAFEVLYVWLVNLGEARSKAIESHPNYLKKYL

Experimental data[edit source | edit]

  • experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]