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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0595 [new locus tag: SACOL_RS03085 ]
  • pan locus tag?: SAUPAN002321000
  • symbol: SACOL0595
  • pan gene symbol?:
  • synonym:
  • product: M20/M25/M40 family peptidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0595 [new locus tag: SACOL_RS03085 ]
  • symbol: SACOL0595
  • product: M20/M25/M40 family peptidase
  • replicon: chromosome
  • strand: -
  • coordinates: 619624..620799
  • length: 1176
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTTAGATTGGTTCCAACTAGCAAATAATAAAGAAAATAAAACAATTCAACTCAGACGT
    TATTTACACCAATATCCTGAGTTATCTTTTGAAGAATTTCAAACACATGATTATATTGTT
    AACCAATTAAGCCAGTTATCTTGTGATATTGAAACTCCTATCGGCCGCAATGGTATCAAA
    GCCACATTTAAAGGCTTGGGCACAGGACCTACCATTGCATTAAGAGCTGACTTTGATGCG
    TTACCTGTCGAGGAATTAAACGATGTGCCTTATAAGTCTAAAAATCCAGGATGTATGCAT
    GCGTGTGGGCATGATGGTCATACAGCAATTTTACTTACTGTAGCAGAGATTTTAGATGAA
    CATAAACATTTATTAGAAGGTAATGTTGTATTAATATTCCAGTACGGTGAAGAAATCATG
    CCAGGTGGTTCTCAAGAAATGATAGATGCTGGATGTCTGGAAAATGTAGATAGAATTTAC
    GGTACACATTTATGGAGTGGTTATCCTACTGGAACTATTCATTCACGTGCGGGGGCAATC
    ATGGCCTCTCCAGATGAATTTAGCGTCACATTTAAAGGTCGTGGTGGTCATGGTGCCAAA
    CCTCATGAAACCATTGACCCAATAGTAATCATGGCAGAGTTCATTTTAAGTGCACAAAAA
    ATAATTTCACGTACGATCGATCCAGTTAAACAAGCTGTACTTTCATTTGGAATGATTCAA
    GCAGGCACCACAGATAGCGTTATTCCTGATCAAGCATTTTGTAAAGGTACAGTTAGAACT
    TTCGATTCAGATATACAGAATCATGTAATGGACAAAATGGATAAGCTTCTACAAGGTTTA
    GCAATAGCAAATGATATTAACTATGATTTGAATTATATTAAAGGGTATTTGCCTGTCCAT
    AATAACGAGAAAGCATATCAAGTTATTAAAGAAGCAACAAATGACTTACATGTACGCTTT
    AATGAATCAGATTTAATGATGATTGGAGAAGATTTTTCACATTATTTGAAAGTACGACCA
    GGTGCATTCTTTCTGACTGGTTGTGGTAATGAAAGTAAAGGTATTACAGCGCCACACCAT
    AATCCCAAATTTGATATTGATGAAAAATCATTAAAATACGCTGTAGCTGTATTTTTAAAA
    ATCATAGAATTAGAGCAAGTATTTAAAACAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0595 [new locus tag: SACOL_RS03085 ]
  • symbol: SACOL0595
  • description: M20/M25/M40 family peptidase
  • length: 391
  • theoretical pI: 5.65496
  • theoretical MW: 43657.4
  • GRAVY: -0.23555

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesamidohydrolase (TIGR01891; HMM-score: 385.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 38)
    MetabolismAmino acid biosynthesisGlutamate familyacetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 30.7)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 26.5)
    MetabolismAmino acid biosynthesisAspartate familysuccinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 24.2)
    MetabolismAmino acid biosynthesisAspartate familysuccinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 21.7)
    MetabolismCentral intermediary metabolismNitrogen metabolismallantoate amidohydrolase (TIGR03176; EC 3.5.3.9; HMM-score: 17.3)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47) 
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 114.2)
    no clan definedM20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 38.3)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 14
    • Cmax: 0.11
    • Cmax_pos: 62
    • Smax: 0.234
    • Smax_pos: 7
    • Smean: 0.134
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLSQLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHACGHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIYGTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSAQKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKLLQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSHYLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 86 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL0595 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]