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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0764 [new locus tag: SACOL_RS03925 ]
  • pan locus tag?: SAUPAN002589000
  • symbol: SACOL0764
  • pan gene symbol?: csbB
  • synonym:
  • product: glycosyl transferase, group 2 family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0764 [new locus tag: SACOL_RS03925 ]
  • symbol: SACOL0764
  • product: glycosyl transferase, group 2 family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 786676..787659
  • length: 984
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATTAGAGTCGTCATTCCTTGTTTTAATGAAGGGGAAGTCATTACACAAACACAT
    CAACAATTAACTGAAATACTTTCACAAGATAGTAGTGTGAAAGGCTATGATTATAATATG
    CTTTTCATAGATGATGGTAGTACGGATACCACTATAGATGAAATGCAACATCTTGCCACA
    ATAGATAGGCATGTCAGCTTTATTTCTTTTAGTAGAAATTTTGGAAAAGAAGCAGCTATG
    ATTGCAGGTTACCAGCATAGTACTGAATTTGATGCAGTCATCATGATAGATTGTGATTTG
    CAACATCCACCTGAATATATTCCGAAAATGGTTGAAGGTTTTATGGAAGGCTATGATCAA
    GTGATTGCAAAGCGTGATAGAAGTGGTGAAAATTTTAGTCGCAAAACATTAAGCCATTTG
    TATTATAAGTTAGTTAATTGCTTTGTAGAAGAAGTACAATTTGATGATGGTGTTGGTGAT
    TTTAGACTTTTAAGCCAAAGAGCTGTTAAATCCATTGCATCACTTGAAGAATATAATCGA
    TTTTCAAAAGGGTTATTTGAATGGATAGGCTATAATACTAAAGTGTTTACGTATCAAAAT
    GTTGAGAGACAAAAAGGGGAATCTAAGTGGTCCTTTAAAAAGTTATTTAATTATGGTATT
    GATGGATTGATTTCCTTTAATAGTAAACCTTTGAGAATGATGATTTATCTTGGCTTGTTT
    ATCTTTTCAATAAGCGTGTTATATATTATCTATTTATTCATCAATATTATGATATCTGGT
    GTTAATATTCCGGGATATTTTTCAACGATTGCAGCTATTTTATTATTAGGCGGCATACAG
    TTAATTTCAATTGGTGTTGTAGGTGAATATATTGGCAGGATATATTATGAAGTTAAGGCA
    CGTCCTAAATATATTATTCAAGCTACAAATCTTTCAAGTCTTGAAAATGATGAGAAGGAT
    ACCCATAAAGTTTATTCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0764 [new locus tag: SACOL_RS03925 ]
  • symbol: SACOL0764
  • description: glycosyl transferase, group 2 family protein
  • length: 327
  • theoretical pI: 5.92494
  • theoretical MW: 37576.9
  • GRAVY: -0.0776758

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 47.8)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 42.7)
    chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 31.8)
    hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 20.3)
    Unknown functionEnzymes of unknown specificitytransferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 20.2)
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 17.4)
  • TheSEED:  
    CsbB stress response protein 
  • PFAM:
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 89.9)
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 30.3)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 18.6)
    Glyco_transf_21; Glycosyl transferase family 21 (PF13506; HMM-score: 14.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.137
    • Ymax_pos: 24
    • Cmax: 0.116
    • Cmax_pos: 24
    • Smax: 0.227
    • Smax_pos: 18
    • Smean: 0.154
    • D: 0.144
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFIDDGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQHPPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGDFRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNYGIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLLGGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]