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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0778 [new locus tag: SACOL_RS04000 ]
  • pan locus tag?: SAUPAN002605000
  • symbol: SACOL0778
  • pan gene symbol?: ltaS
  • synonym:
  • product: sulfatase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0778 [new locus tag: SACOL_RS04000 ]
  • symbol: SACOL0778
  • product: sulfatase
  • replicon: chromosome
  • strand: +
  • coordinates: 798690..800630
  • length: 1941
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGTTCACAAAAAAAGAAAATTAGTCTTTTTGCGTTCTTCTTATTAACCGTAATAACG
    ATTACCTTGAAGACGTATTTTTCTTATTATGTTGATTTTTCTTTAGGTGTTAAAGGTTTA
    GTACAAAACTTAATATTATTGATGAATCCTTATAGTTTAGTAGCACTGGTTTTAAGTGTG
    TTCCTATTCTTTAAAGGCAAAAAAGCATTTTGGTTCATGTTCATAGGCGGCTTCTTATTG
    ACGTTCCTATTATATGCCAATGTTGTGTACTTTAGATTCTTCTCTGATTTTTTAACGTTT
    AGTACTTTAAACCAAGTAGGTAACGTAGAATCTATGGGTGGTGCGGTTAGTGCATCATTC
    AAATGGTATGACTTTGTTTATTTCATTGATACGTTAGTTTACTTATTCATTTTAATATTT
    AAAACAAAATGGTTAGACACAAAAGCATTTAGTAAGAAATTTGTTCCTGTCGTAATGGCG
    GCTTCAGTAGCATTATTCTTCTTAAACTTAGCTTTTGCTGAAACTGACAGACCAGAATTA
    TTAACACGTACATTTGACCATAAATATTTAGTGAAATATTTAGGACCTTATAACTTTACA
    GTATACGATGGTGTTAAAACTATCGAAAATAATCAACAAAAAGCGCTAGCATCTGAAGAT
    GACTTAACAAAAGTATTAAATTATACGAAACAACGTCAAACAGAGCCTAACCCAGAATAT
    TATGGGGTGGCAAAGAAGAAAAATATTATTAAGATTCATTTAGAAAGTTTCCAAACCTTC
    TTAATTAATAAAAAGGTTAATGGTAAAGAAGTAACACCGTTTTTAAACAAATTATCAAGT
    GGGAAAGAGCAATTCACATACTTCCCTAACTTTTTCCATCAAACAGGTCAAGGTAAAACA
    TCTGACTCTGAATTTACAATGGATAACAGTTTATACGGTTTACCGCAAGGTTCTGCCTTT
    TCATTAAAAGGAGATAATACGTATCAGTCATTACCAGCAATTTTAGATCAAAAGCAAGGC
    TACAAATCTGATGTCATGCACGGTGACTATAAAACATTCTGGAACAGAGACCAAGTATAT
    AAACACTTTGGTATCGATAAATTCTATGATGCAACATACTATGACATGTCAGATAAAAAC
    GTTGTAAACTTAGGCTTGAAAGACAAAATTTTCTTTAAAGATTCTGCTAATTATCAAGCT
    AAGATGAAATCACCATTCTATTCTCATTTAATTACATTGACTAACCACTATCCATTCACA
    TTAGATGAAAAGGATGCAACTATTGAGAAATCAAACACAGGTGATGCAACAGTTGATGGT
    TATATTCAAACAGCACGTTATTTAGACGAAGCATTAGAAGAATATATTAATGACTTGAAG
    AAAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCT
    GAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTT
    ACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAAT
    AATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGAT
    ACGAAGAACTATTTAATGTTCGGTACTGATTTATTCTCTAAAGGTCATAATCAAGTAGTT
    CCATTCAGAAATGGTGACTTTATAACAAAAGATTATAAATATGTTAATGGTAAGATTTAT
    TCTAATAAAAATAATGAACTCATAACTACTCAACCAGCTGATTTCGAAAAGAATAAAAAG
    CAAGTTGAAAAGGATCTCGAAATGAGTGACAACGTGCTTAATGGTGATTTGTTTAGATTC
    TACAAAAATCCAGACTTCAAAAAGGTAAATCCTTCGAAGTATAAATATGAAACAGGACCT
    AAAGCAAACTCTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1941

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0778 [new locus tag: SACOL_RS04000 ]
  • symbol: SACOL0778
  • description: sulfatase
  • length: 646
  • theoretical pI: 9.4708
  • theoretical MW: 74399.6
  • GRAVY: -0.317647

Function[edit | edit source]

  • reaction:
    EC 2.7.8.-?  ExPASy
  • TIGRFAM:
    choline-sulfatase (TIGR03417; EC 3.1.6.6; HMM-score: 20.4)
    and 1 more
    frataxin (TIGR03422; HMM-score: 12.3)
  • TheSEED  :
    • Lipoteichoic acid synthase LtaS Type Ib
    Cell Wall and Capsule Gram-Positive cell wall components Polyglycerolphosphate lipoteichoic acid biosynthesis  Lipoteichoic acid synthase LtaS Type Ib
  • PFAM:
    Alk_phosphatase (CL0088) Sulfatase; Sulfatase (PF00884; HMM-score: 113)
    and 3 more
    DUF229; Protein of unknown function (DUF229) (PF02995; HMM-score: 19.8)
    Phosphodiest; Type I phosphodiesterase / nucleotide pyrophosphatase (PF01663; HMM-score: 18.8)
    DUF1501; Protein of unknown function (DUF1501) (PF07394; HMM-score: 13.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008585
    • TAT(Tat/SPI): 0.000154
    • LIPO(Sec/SPII): 0.048829
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNLILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFSTLNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMAASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALASEDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLNKLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAILDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFFKDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDEALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGFWIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGDFITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNPDFKKVNPSKYKYETGPKANSKK

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]