From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0918 [new locus tag: SACOL_RS04710 ]
  • pan locus tag?: SAUPAN002899000
  • symbol: sufB
  • pan gene symbol?: sufB
  • synonym:
  • product: FeS assembly protein SufB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0918 [new locus tag: SACOL_RS04710 ]
  • symbol: sufB
  • product: FeS assembly protein SufB
  • replicon: chromosome
  • strand: +
  • coordinates: 925370..926767
  • length: 1398
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTAAAAAAGCACCTGATGTTGGGGATTATAAATATGGATTCCACGACGATGATGTG
    TCCATTTTCAGATCAGAACGTGGTTTAACTGAGAATATCGTTAGAGAAATTTCTAACATG
    AAAAATGAGCCGGAATGGATGTTAGATTTCCGTCTTAAATCATTAAAATTGTTTTATAAA
    ATGCCAATGCCTCAATGGGGTGGCGACTTATCAGAATTGAATTTCGATGACATTACTTAC
    TATGTAAAGCCTTCAGAACAAGCTGAACGTTCATGGGATGAAGTGCCAGAAGAAATTAAA
    AGAACTTTCGATAAATTAGGAATTCCTGAAGCTGAACAAAAATATTTAGCTGGTGTTTCT
    GCTCAATATGAATCTGAAGTTGTTTACCATAATATGGAAAAAGAACTTGAAGAAAAAGGT
    ATTATCTTTAAAGATACAGATAGTGCTTTACAAGAAAATGAAGAATTATTCAAAAAATAC
    TTTGCTTCTGTAGTACCTGCAGCAGATAACAAATTTGCGGCGTTAAACTCAGCAGTATGG
    TCAGGTGGTTCGTTCATTTATGTACCTAAAAATATCAAACTAGATACGCCACTACAAGCT
    TATTTCCGTATTAACTCTGAGAACATGGGTCAATTTGAACGTACATTAATCATTGCTGAT
    GAAGGTGCTTCTGTACATTACGTAGAAGGTTGTACTGCACCAGTTTATACAACTAGTTCT
    TTACACTCTGCTGTTGTGGAAATCATTGTGCATAAAGATGCGCACGTTCGTTATACTACG
    ATTCAAAACTGGGCGAACAATGTATACAATTTAGTTACAAAACGTACTTTTGTTTATGAA
    AACGGAAATATGGAATGGGTAGATGGTAACTTAGGTTCTAAGTTAACGATGAAATATCCA
    AACTGTGTTCTTTTAGGTGAAGGTGCAAAAGGTAGTACATTATCTATTGCATTTGCTGGT
    AAAGGACAAGTTCAAGATGCCGGTGCTAAAATGATTCATAAAGCACCTAATACATCTTCT
    ACAATTGTTTCTAAATCTATTTCTAAAAATGGTGGTAAAGTTATTTATCGCGGTATTGTT
    CATTTTGGACGTAAAGCAAAAGGTGCTCGTTCAAATATTGAATGTGATACATTAATCTTA
    GATAACGAATCAACATCAGATACAATTCCATATAACGAAGTATTCAACGATCAAATATCA
    TTAGAACATGAAGCCAAGGTTTCAAAAGTTTCTGAAGAACAATTATTCTATCTAATGAGT
    CGTGGTATTTCTGAAGAAGAAGCGACAGAAATGATTGTTATGGGATTCATCGAACCATTT
    ACAAAAGAACTTCCAATGGAATACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATG
    GAAGGTAGTATTGGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1398

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0918 [new locus tag: SACOL_RS04710 ]
  • symbol: SufB
  • description: FeS assembly protein SufB
  • length: 465
  • theoretical pI: 4.81321
  • theoretical MW: 52531.1
  • GRAVY: -0.397849

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherFeS assembly protein SufB (TIGR01980; HMM-score: 767.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherFeS assembly protein SufD (TIGR01981; HMM-score: 201.1)
  • TheSEED:  
    Sulfur MetabolismOrganic sulfur assimilationAt5g37530 (CsdL protein family) Iron-sulfur cluster assembly protein SufB 
  • PFAM:
    no clan definedUPF0051; Uncharacterized protein family (UPF0051) (PF01458; HMM-score: 250.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 70
    • Cmax: 0.105
    • Cmax_pos: 5
    • Smax: 0.117
    • Smax_pos: 66
    • Smean: 0.065
    • D: 0.085
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEPEWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKYFASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLIIADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRTFVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHKAPNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYNEVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 2591 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SACOL1919 (PerR*)
    PerR* (TF) important in Oxidative stress responseRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 7.07 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]