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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0970 [new locus tag: SACOL_RS04970 ]
  • pan locus tag?: SAUPAN003076000
  • symbol: rexB
  • pan gene symbol?: addB
  • synonym:
  • product: exonuclease RexB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0970 [new locus tag: SACOL_RS04970 ]
  • symbol: rexB
  • product: exonuclease RexB
  • replicon: chromosome
  • strand: +
  • coordinates: 971982..975458
  • length: 3477
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACATTACATGCTTATTTAGGTAGAGCGGGAACAGGTAAGTCTACGAAAATGTTGACC
    GAAATAAAACAAAAAATGAAAGCAGATCCGCTTGGAGATCCAATCATTTTAATTGCGCCA
    ACTCAAAGTACATTTCAATTAGAACAAGCCTTTGTCAATGATCCGGAATTAAATGGTAGT
    TTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGT
    AGTTATAGCGAACAAAAGTTATCTAAAGCTGCAACGGAAATGATGATTTATAACATTGTT
    CAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGT
    GAAAAATTAACAGAACAAATTCAAGATTTTAAAAAATATGCAGTAACGCCTGAACATTTA
    GAACACTTTATTGCTGATAAAAATATGCAAACTCGAACTAAAAATAAGTTAGAGGATATT
    GCTTTAATATACCGTGAGTTCGAACAACGCATTCAAAACGAGTTTATTACGGGTGAGGAT
    TCATTACAATATTTTATTGATTGTATGCCGAAATCAGAGTGGCTAAAACGTGCTGATATA
    TATATTGATGGTTTTCACAACTTTTCAACGATTGAGTATTTAATAATCAAAGGATTAATT
    AAATATGCGAAGAGTGTCACAATTATATTGACGACAGATGGTAACCACGATCAATTTAGT
    TTATTTAGAAAACCATCGGAAGTGTTACGACATATTGAAGAAATAGCAAATGAACTCAAT
    ATTTCTATTGAACGTCAATATTTCAACCAATTATATCGCTTCAATAATCAAGATTTAAAG
    CATCTTGAACAAGAATTTGATGCACTTCAAATCAATCGAGTGGCATGTCAAGGTCATATC
    AATATTTTAGAATCTGCGACTATGAGAGAGGAAATAAATGAAATTGCGCGACGTATCATC
    GTTGATATTCGTGATAAGCAATTACGATATCAAGATATTGCTATTTTATATCGTGATGAA
    TCTTATGCTTATTTATTTGATTCCATATTACCGCTTTATAATATTCCTTATAATATTGAT
    ACAAAGCGTTCGATGACACATCATCCGGTCATGGAAATGATTCGTTCATTGATTGAAGTT
    ATTCAATCTAATTGGCAAGTGAATCCAATGCTACGCTTATTGAAGACTGATGTGTTAACG
    GCATCATATCTAAAAAGTGCATACTTAGTTGATTTACTTGAAAATTTTGTACTTGAACGT
    GGTATATACGGTAAACGTTGGTTAGATGATGAGCTATTTAATGTCGAACATTTTAGCAAA
    ATGGGGCGTAAAGGGCATAAACTGACCGAAGATGAACGTAACACATTTGAACAAGTCGTT
    AAGTTAAAGAAAGATGTCATTGATAAAATTTTACATTTTGAAAAGCAAATGTCACAAGCG
    GAAACTGTAAAAGATTTTGCAACTGCTTTTTATGAAAGTATGGAATATTTCGAACTGCCA
    AATCAATTGATGACAGAGCGAGATGAACTTGATTTAAATGGTAATCATGAAAAGGCGGAG
    GAAATTGATCAAATATGGAATGGCTTAATTCAAATCCTTGATGACTTAGTTCTAGTATTT
    GGAGATGAACCAATGTCGATGGAACGTTTCTTAGAAGTATTTGATATTGGTTTAGAACAA
    TTAGAATTTGTTATGATTCCGCAAACATTGGACCAAGTAAGTATTGGTACGATGGATTTG
    GCTAAAGTCGATAATAAGCAACATGTTTACTTAGTAGGTATGAATGATGGAACGATGCCA
    CAACCAGTAACTGCGTCAAGCTTGATTACAGATGAAGAAAAGAAATACTTTGAACAGCAG
    GCTAATGTCGAGTTAAGTCCAACATCAGATATTTTACAGATGGATGAAGCATTTGTTTGT
    TATGTTGCTATGACTAGAGCTAAGGGAGATGTTACATTTTCTTACAGTCTAATGGGATCA
    AGTGGTGATGATAAGGAGATCAGCCCATTTTTAAATCAAATTCAATCATTGTTCAACCAA
    TTGGAAATTACTAACATTCCTCAATACCATGAAGTTAACCCATTGTCACTAATGCAACAT
    GCTAAGCAAACCAAAATTACATTATTTGAAGCATTGCGTGCTTGGTTATATGATGAAATT
    GTGGCTGATAGTTGGTTAGATGCTTATCAAGTAATTAGAGATAGCGATCATTTAAATCAA
    GGTTTAGATTATTTAATGTCAGCATTAACGTTTGACAATGAAACTGTAAAATTAGGTGAA
    ACGTTGTCTAAAGATTTATATGGTAAGGAAATCAATGCCAGTGTATCCCGTTTTGAAGGT
    TATCAACAATGCCCATTTAAACACTATGCGTCACATGGTCTGAAACTAAATGAGCGAACG
    AAGTATGAACTTCAAAACTTTGATTTAGGTGATATTTTCCATTCTGTTTTAAAATATATA
    TCTGAACGTATTAATGGCGATTTTAAACAATTAGACCTGAAAAAAATAAGACAATTAACG
    AATGAAGCATTGGAAGAAATTTTACCTAAAGTTCAGTTTAATTTATTAAATTCTTCAGCT
    TACTATCGTTATTTATCAAGACGCATTGGCGCTATTGTAGAAACAACACTAAGCGCATTA
    AAATATCAAGGCACGTATTCAAAGTTTATGCCAAAACATTTTGAGACAAGTTTTAGAAGG
    AAACCAAGAACAAATGACGAATTAATTGCACAAACATTAACGACAACTCAAGGTATTCCA
    ATTAATATTAGAGGGCAAATTGACCGTATCGATACGTATACAAAGAATGATACAAGTTTT
    GTTAATATCATTGACTATAAATCCTCTGAAGGTAGTGCGACACTTGATTTAACGAAAGTA
    TATTATGGTATGCAAATGCAAATGATGACATACATGGATATCGTTTTACAAAATAAACAA
    CGCCTTGGATTAACAGATATTGTGAAACCAGGTGGATTATTATACTTCCATGTACATGAA
    CCTAGAATTAAATTTAAATCATGGTCTGATATTGATGAAGATAAACTAGAACAAGATTTA
    ATTAAAAAGTTTAAGTTGAGTGGTTTAGTTAATGCAGACCAAACTGTTATTGATGCATTG
    GATATTCGTTTAGAACCTAAATTCACTTCAGATATTGTACCAGTTGGTTTGAATAAAGAT
    GGCTCTTTGAGTAAACGAGGCAGCCAAGTGGCAGATGAAGCAACGATTTATAAATTCATC
    CAACATAACAAAGAGAATTTTATAGAAACAGCTTCAAATATTATGGATGGACATACTGAA
    GTTGCACCATTAAAGTACAAACAAAAATTGCCATGTGCTTTTTGTAGTTATCAATCGGTA
    TGTCATGTAGATGGCATGATTGATAGTAAGCGATATCGAACTGTAGATGAAACAATAAAT
    CCAATTGAAGCAATTCAAAATATTAACATTAATGATGAATTTGGGGGTGAGCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3477

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0970 [new locus tag: SACOL_RS04970 ]
  • symbol: RexB
  • description: exonuclease RexB
  • length: 1158
  • theoretical pI: 5.06875
  • theoretical MW: 134490
  • GRAVY: -0.423575

Function[edit source | edit]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicaseATP + H2O = ADP + phosphate
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairhelicase-exonuclease AddAB, AddB subunit (TIGR02773; EC 3.1.-.-,3.6.4.12; HMM-score: 1417.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent nuclease subunit B (TIGR02774; HMM-score: 173.6)
    probable DNA repair protein (TIGR03623; HMM-score: 32.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 30.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairdouble-strand break repair protein AddB (TIGR02786; HMM-score: 30)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 14.2)
  • TheSEED:  
    DNA MetabolismDNA repairATP-dependent Nuclease ATP-dependent nuclease, subunit B 
  • PFAM:
    PDDEXK (CL0236) PDDEXK_1; PD-(D/E)XK nuclease superfamily (PF12705; HMM-score: 174.4)
    P-loop_NTPase (CL0023) UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 58.6)
    AAA_30; AAA domain (PF13604; HMM-score: 14.5)
    no clan definedImm21; Immunity protein 21 (PF15589; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.151
    • Ymax_pos: 12
    • Cmax: 0.151
    • Cmax_pos: 29
    • Smax: 0.311
    • Smax_pos: 3
    • Smean: 0.216
    • D: 0.177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQSTFQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQEQQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDIALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKGLIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNNQDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIAILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLRLLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKGHKLTEDERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERDELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMIPQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVELSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLEITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQGLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNERTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLLNSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLTTTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYMDIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLVNADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFIETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQNININDEFGGEQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 90.52 h [4]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]