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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1074 [new locus tag: SACOL_RS05480 ]
  • pan locus tag?: SAUPAN003278000
  • symbol: purK
  • pan gene symbol?: purK
  • synonym:
  • product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1074 [new locus tag: SACOL_RS05480 ]
  • symbol: purK
  • product: phosphoribosylaminoimidazole carboxylase ATPase subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 1083391..1084515
  • length: 1125
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACTTCAATAAATTAAAGTTTGGTGCGACTATTGGCATTATTGGTGGTGGTCAGCTT
    GGAAAGATGATGGCACAATCAGCTCAAAAAATGGGTTATAAAGTGGTTGTATTGGATCCT
    TCTGAAGATTGTCCATGTAGATACGTTGCACACGAATTTATACAAGCCAAGTATGACGAT
    GAAAAGGCACTCAATCAATTAGGACAAAAATGTGATGTGATTACTTATGAATTTGAAAAC
    ATTTCAGCCCAACAATTAAAACTATTATGTGAAAAGTACAATATTCCGCAAGGTTACCAA
    GCTATACAGTTATTACAAGATCGCTTAACTGAAAAAGAAACATTAAAAAGTGCTGGTACC
    AAAGTTGTCCCGTTCATTTCAGTAAAAGAATCTACAGATATTGACAAAGCAATTGAAACA
    TTAGGATATCCTTTTATTGTAAAAACTAGATTTGGTGGCTACGATGGCAAAGGTCAAGTT
    TTAATTAACAACGAAAAAGACTTACAAGAAGGTTTTAAATTAATTGAAACTAGTGAATGC
    GTAGCTGAAAAATATTTGAATATCAAGAAAGAAGTATCTCTTACTGTTACAAGAGGAAAC
    AACAATCAAATCACTTTTTTCCCATTACAAGAAAATGAGCATAGAAATCAAATACTTTTC
    AAAACAATTGTTCCAGCGAGAATAGATAAAACAGCTGAGGCGAAAGAGCAAGTTAATAAA
    ATTATCCAATCGATTCATTTCATTGGAACATTTACAGTTGAATTTTTTATAGATAGTAAC
    AACCAATTGTATGTGAACGAGATAGCACCAAGGCCTCACAATTCCGGACATTATTCAATT
    GAAGCATGTGATTATTCACAATTTGATACTCATATTTTAGCAGTTACCGGACAATCATTA
    CCAAATTCAATTGAATTATTAAAGCCAGCAGTCATGATGAACTTACTAGGTAAAGATTTA
    GATTTATTGGAAAATGAATTTAATGAACATCCAGAGTGGCACTTACATATTTATGGTAAG
    TCTGAGCGTAAAGATAGCAGAAAAATGGGGCATATGACTGTACTAACGAATGATGTAAAC
    CAAACTGAACAAGATATGTACGCTAAATTTGAGGGGAGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

Protein[edit source | edit]

Protein Data Bank: 3ORQ
Protein Data Bank: 3ORR

General[edit source | edit]

  • locus tag: SACOL1074 [new locus tag: SACOL_RS05480 ]
  • symbol: PurK
  • description: phosphoribosylaminoimidazole carboxylase ATPase subunit
  • length: 374
  • theoretical pI: 5.8331
  • theoretical MW: 42493.1
  • GRAVY: -0.437968

Function[edit source | edit]

  • reaction:
    EC 6.3.4.18?  ExPASy
    5-(carboxyamino)imidazole ribonucleotide synthaseATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
    EC 4.1.1.21?  ExPASy
    Phosphoribosylaminoimidazole carboxylase5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 385.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 112.8)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 38.7)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 37.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesiscarbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 37.1)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 35.9)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesispyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 29.9)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 23.4)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 20.4)
    MetabolismAmino acid biosynthesisSerine familyphosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 18.8)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 18.4)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 11.9)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesDe Novo Purine Biosynthesis Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21) 
  • PFAM:
    ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 160.9)
    CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 48.3)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 27.1)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 27)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 24.5)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 23.5)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 21.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20.7)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 19.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.6)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 16.5)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 16.4)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 15.8)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 15)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.3)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 13.7)
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 13.2)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 12.9)
    ATP-grasp (CL0179) ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 12.5)
    DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 12.3)
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 12.2)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.303
    • Ymax_pos: 28
    • Cmax: 0.316
    • Cmax_pos: 28
    • Smax: 0.396
    • Smax_pos: 24
    • Smean: 0.284
    • D: 0.296
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQVLINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 281 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]