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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1251 [new locus tag: SACOL_RS06390 ]
  • pan locus tag?: SAUPAN003522000
  • symbol: SACOL1251
  • pan gene symbol?: ftsY
  • synonym:
  • product: cell division protein FtsY

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1251 [new locus tag: SACOL_RS06390 ]
  • symbol: SACOL1251
  • product: cell division protein FtsY
  • replicon: chromosome
  • strand: +
  • coordinates: 1260104..1261354
  • length: 1251
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTT
    AAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCA
    ACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTG
    AGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAA
    GCTCAAAAAATGGGTGCTAAATTTAAAGCAGGACTCGAAAAATCTCGTCAAAATTTCCAA
    GAACAATTAAATAATTTGATAGCGAGATATCGTAAAGTAGATGAAGACTTTTTTGAAGCT
    TTAGAAGAAATGTTAATCACTGCAGACGTCGGTTTTAATACAGTGATGACGTTAACTGAA
    GAATTACGTATGGAAGCACAACGACGTAATATTCAAGATACTGAAGATTTGCGTGAAGTC
    ATTGTTGAAAAAATCGTAGAGATTTACCATCAAGAAGATGATAATTCAGAAGCTATGAAC
    TTAGAAGATGGTCGTTTAAATGTCATTTTAATGGTTGGTGTGAATGGTGTTGGTAAAACA
    ACAACAATTGGAAAATTAGCTTACCGATATAAAATGGAAGGTAAAAAAGTAATGTTAGCT
    GCGGGCGATACTTTTAGAGCGGGTGCTATTGATCAATTGAAAGTTTGGGGCGAACGTGTT
    GGTGTAGACGTAATTAGCCAAAGTGAAGGTTCTGATCCAGCTGCTGTTATGTATGATGCG
    ATTAATGCCGCTAAAAACAAAGGTGTTGATATTTTAATCTGTGATACCGCTGGACGTTTA
    CAAAATAAAACAAATCTAATGCAAGAATTAGAAAAAGTTAAGCGTGTAATTAATCGAGCA
    GTGCCAGATGCGCCTCATGAAGCATTACTATGTTTAGATGCTACAACTGGTCAGAATGCG
    TTGTCACAAGCTAGAAACTTTAAAGAAGTAACAAATGTTACAGGTATTGTATTAACGAAA
    TTAGATGGTACAGCCAAAGGTGGTATCGTATTAGCCATTCGTAATGAATTGCACATCCCA
    GTTAAATATGTAGGTTTAGGTGAGCAATTAGATGACTTACAACCATTTAACCCTGAAAGT
    TATGTCTACGGCTTATTCGCTGATATGATTGAACAAAATGAAGAAATAACAACAGTTGAA
    AATGATCAAATTGTAACAGAAGAAAAGGACGATAATCATGGGTCAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1251 [new locus tag: SACOL_RS06390 ]
  • symbol: SACOL1251
  • description: cell division protein FtsY
  • length: 416
  • theoretical pI: 4.39014
  • theoretical MW: 46590
  • GRAVY: -0.545673

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingsignal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 388.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingsignal recognition particle protein (TIGR00959; HMM-score: 233.6)
    signal recognition particle protein SRP54 (TIGR01425; HMM-score: 185.8)
    Cellular processesCellular processesChemotaxis and motilityflagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 91.4)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 18.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesLAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)
    Signal transductionRegulatory functionsProtein interactionsLAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)
    arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16)
    Genetic information processingProtein fateProtein modification and repairhydrogenase accessory protein HypB (TIGR00073; HMM-score: 15.7)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 13.2)
    Unknown functionGeneralputative selenium-dependent hydroxylase accessory protein YqeC (TIGR03172; HMM-score: 12.9)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 12)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryTwo cell division clusters relating to chromosome partitioning Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1) 
    Protein MetabolismProtein biosynthesisUniversal GTPases Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1) 
    Bacterial Cell Division Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1) 
  • PFAM:
    P-loop_NTPase (CL0023) SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 273.5)
    no clan definedSRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 48.9)
    P-loop_NTPase (CL0023) ArgK; ArgK protein (PF03308; HMM-score: 19)
    AAA_30; AAA domain (PF13604; HMM-score: 18.6)
    AAA_17; AAA domain (PF13207; HMM-score: 16.9)
    ABC_tran; ABC transporter (PF00005; HMM-score: 15.5)
    AAA_31; AAA domain (PF13614; HMM-score: 15.2)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 15.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.7)
    AAA_22; AAA domain (PF13401; HMM-score: 14.5)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.3)
    AAA_33; AAA domain (PF13671; HMM-score: 14.1)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.4)
    AAA_24; AAA domain (PF13479; HMM-score: 13.2)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 12.8)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 9.5)
    no clan definedScm3; Centromere protein Scm3 (PF10384; HMM-score: 6.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL0452(ahpC)alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    SACOL0842(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    SACOL1329(femC)glutamine synthetase  [1] (data from MRSA252)
    SACOL1734(gapA2)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    SACOL1741(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SACOL1274(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SACOL1449(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [1] (data from MRSA252)
    SACOL2104(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SACOL2553pyruvate oxidase  [1] (data from MRSA252)
    SACOL2561hydroxymethylglutaryl-CoA synthase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.165
    • Ymax_pos: 13
    • Cmax: 0.107
    • Cmax_pos: 21
    • Smax: 0.311
    • Smax_pos: 11
    • Smean: 0.259
    • D: 0.202
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDANDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQLNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREVIVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVMLAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDTAGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVTGIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQNEEITTVENDQIVTEEKDDNHGSK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3] [4] [5]
    quantitative data / protein copy number per cell: 543 [6]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 65.44 h [7]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  4. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  7. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]