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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1283 [new locus tag: SACOL_RS06550 ]
  • pan locus tag?: SAUPAN003565000
  • symbol: polC
  • pan gene symbol?: polC
  • synonym:
  • product: DNA polymerase III PolC

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1283 [new locus tag: SACOL_RS06550 ]
  • symbol: polC
  • product: DNA polymerase III PolC
  • replicon: chromosome
  • strand: +
  • coordinates: 1293299..1297609
  • length: 4311
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTA
    GATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGA
    ACATGGGAATTTCATATTACATTACCACAATTCTTAGCTCATGAAGATTATTTATTATTT
    ATAAATGCAATAGAGCAAGAGTTTAAAGATATCGCCAACGTTACATGTCGTTTTACGGTA
    ACAAATGGCACGAATCAAGATGAACATGCAATTAAATACTTTGGGCACTGTATTGACCAA
    ACAGCTTTATCTCCAAAAGTTAAAGGTCAATTGAAACAGAAAAAGCTTATTATGTCTGGA
    AAAGTATTAAAAGTAATGGTATCAAATGACATTGAACGTAATCATTTTGATAAGGCATGT
    AATGGAAGTCTTATCAAAGCGTTTAGAAATTGTGGTTTTGATATCGATAAAATCATATTC
    GAAACAAATGATAATGATCAAGAACAAAACTTAGCTTCTTTAGAAGCACATATTCAAGAA
    GAAGACGAACAAAGTGCACGATTGGCAACAGAGAAACTTGAAAAAATGAAAGCTGAAAAA
    GCGAAACAACAAGATAACAACGAAAGTGCTGTCGATAAGTGTCAAATTGGTAAGCCGATT
    CAAATTGAAAATATTAAACCAATTGAATCTATTATTGAGGAAGAGTTTAAAGTTGCAATA
    GAGGGTGTCATTTTTGATATAAACTTAAAAGAACTTAAAAGTGGTCGCCATATCGTAGAA
    ATTAAAGTGACTGACTATACGGACTCTTTAGTTTTAAAAATGTTTACTCGTAAAAACAAA
    GATGATTTAGAACATTTTAAAGCGCTAAGTGTTGGTAAATGGGTTAGGGCTCAAGGTCGT
    ATTGAAGAAGATACATTTATTAGAGATTTAGTTATGATGATGTCTGATATTGAAGAGATT
    AAAAAAGCGACAAAAAAAGATAAGGCTGAAGAAAAGCGTGTAGAATTCCACTTGCATACT
    GCAATGAGCCAAATGGATGGTATACCCAATATTGGTGCGTATGTTAAACAGGCAGCAGAC
    TGGGGACATCCAGCCATTGCGGTTACAGACCATAATGTTGTGCAAGCATTTCCAGATGCT
    CACGCAGCAGCGGAAAAACATGGCATTAAAATGATATACGGTATGGAAGGTATGTTAGTT
    GATGATGGTGTTCCGATTGCATACAAACCACAAGATGTCGTATTAAAAGATGCTACTTAT
    GTTGTGTTCGACGTTGAGACAACTGGTTTATCAAATCAGTATGATAAAATCATCGAGCTT
    GCAGCTGTGAAAGTTCATAACGGTGAAATCATCGATAAGTTTGAAAGGTTTAGTAATCCG
    CATGAACGATTATCGGAAACGATTATCAATTTGACGCATATTACTGATGATATGTTAGTA
    GATGCCCCTGAGATTGAAGAAGTACTTACAGAGTTTAAAGAATGGGTTGGCGATGCGATA
    TTCGTAGCGCATAATGCTTCGTTTGATATGGGCTTCATCGATACGGGATATGAACGTCTT
    GGGTTTGGACCATCAACGAATGGTGTTATCGATACTTTAGAATTATCTCGTACGATTAAT
    ACTGAATATGGTAAACATGGTTTGAATTTCTTGGCTAAAAAATATGGCGTAGAATTAACG
    CAACATCACCGTGCCATTTATGATACAGAAGCAACAGCTTACATTTTCATAAAAATGGTT
    CAACAAATGAAAGAATTAGGCGTATTAAATCATAACGAAATCAACAAAAAACTCAGTAAT
    GAAGATGCATATAAACGTGCAAGACCTAGTCATGTCACATTAATTGTACAAAACCAACAA
    GGTCTTAAAAATCTATTTAAAATTGTAAGTGCATCATTGGTGAAGTATTTCTACCGTACA
    CCTCGAATTCCACGTTCATTGTTAGATGAATATCGTGAGGGATTATTGGTAGGTACAGCG
    TGTGATGAAGGTGAATTATTTACGGCAGTTATGCAGAAGGACCAGAGTCAAGTTGAAAAA
    ATTGCCAAATATTATGATTTTATTGAAATTCAACCACCGGCACTTTATCAAGATTTAATT
    GATAGAGAGCTTATTAGAGATACTGAAACATTACATGAAATTTATCAACGTTTAATACAT
    GCAGGTGACACAGCGGGTATACCTGTTATTGCGACAGGAAATGCACACTATTTGTTTGAA
    CATGATGGTATCGCACGTAAAATTTTAATAGCATCACAACCCGGCAATCCACTTAATCGC
    TCAACTTTACCGGAAGCACATTTTAGAACTACAGATGAAATGTTAAACGAGTTTCATTTT
    TTAGGTGAAGAAAAAGCGCATGAAATTGTTGTGAAAAATACAAACGAATTAGCAGATCGA
    ATTGAACGTGTTGTTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAAC
    GAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTGAAGACCTGCCT
    CAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCG
    GTAATTTACTTAATTTCGCAACGTTTAGTTAAAAAATCATTAGATGATGGATACTTAGTT
    GGTTCCCGTGGTTCAGTAGGTTCTAGTTTTGTAGCGACAATGACTGAGATTACTGAAGTA
    AACCCGTTACCGCCACACTATATTTGTCCGAACTGTAAAACGAGTGAATTTTTCAATGAT
    GGTTCAGTAGGATCAGGATTTGATTTACCTGATAAGACGTGTGAAACTTGTGGAGCGCCA
    CTTATTAAAGAAGGACAAGATATTCCGTTTGAAACATTTTTAGGATTTAAGGGAGATAAA
    GTTCCTGATATCGACTTAAACTTTAGTGGTGAATATCAACCGAATGCCCATAACTACACA
    AAAGTATTATTTGGTGAGGATAAAGTATTCCGTGCAGGTACAATTGGTACTGTTGCTGAA
    AAGACTGCTTTTGGTTATGTTAAAGGTTATTTGAATGATCAAGGTATCCACAAAAGAGGT
    GCTGAAATAGATCGACTCGTTAAAGGATGTACAGGTGTTAAACGTACAACTGGACAGCAT
    CCAGGGGGTATTATTGTAGTACCTGATTACATGGATATTTATGATTTTACGCCGATACAA
    TATCCTGCCGATGATCAAAATTCAGCATGGATGACGACACATTTTGATTTCCATTCTATT
    CATGATAATGTATTAAAACTTGATATACTTGGACACGATGATCCAACAATGATTCGTATG
    CTTCAAGATTTATCAGGAATTGATCCAAAAACAATACCTGTAGATGATAAAGAAGTTATG
    CAGATATTTAGTACACCTGAAAGTTTGGGTGTTACTGAAGATGAAATTTTATGTAAAACA
    GGTACATTTGGGGTACCAGAATTCGGTACAGGATTCGTGCGTCAAATGTTAGAAGATACA
    AAGCCAACAACATTTTCTGAATTAGTTCAAATCTCAGGATTATCTCATGGTACAGATGTG
    TGGTTAGGCAATGCTCAAGAATTAATTAAAACCGGTATATGTGATTTATCAAGTGTAATT
    GGTTGTCGTGATGATATCATGGTTTATTTAATGTATGCTGGTTTAGAACCATCAATGGCT
    TTTAAAATAATGGAGTCAGTACGTAAAGGTAAAGGTTTAACTGAAGAAATGATTGAAACG
    ATGAAAGAAAATGAAGTGCCAGATTGGTATTTAGATTCATGTCTTAAAATTAAGTACATG
    TTCCCTAAAGCCCATGCAGCAGCATACGTTTTAATGGCAGTACGTATCGCATATTTCAAA
    GTACATCATCCACTTTATTACTATGCATCTTACTTTACAATTCGTGCGTCAGACTTTGAT
    TTAATCACGATGATTAAAGATAAAACAAGCATTCGAAATACTGTAAAAGACATGTATTCT
    CGCTATATGGATCTAGGTAAAAAAGAAAAAGACGTATTAACAGTCTTGGAAATTATGAAT
    GAAATGGCGCATCGAGGTTATCGAATGCAACCGATTAGTTTAGAAAAGAGTCAGGCGTTC
    GAATTTATCATTGAAGGCGATACACTTATTCCGCCGTTCATATCAGTGCCTGGGCTTGGC
    GAAAACGTTGCGAAACGAATTGTTGAAGCTCGTGACGATGGCCCATTTTTATCAAAAGAA
    GATTTAAACAAAAAAGCTGGATTATCTCAGAAAATTATTGAGTATTTAGATGAGTTAGGC
    TCATTACCGAATTTACCAGATAAAGCTCAACTTTCGATATTTGATATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4311

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1283 [new locus tag: SACOL_RS06550 ]
  • symbol: PolC
  • description: DNA polymerase III PolC
  • length: 1436
  • theoretical pI: 5.0377
  • theoretical MW: 162459
  • GRAVY: -0.325905

Function[edit source | edit]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymeraseDeoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 2033.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 217.9)
    Genetic information processingDNA metabolismDegradation of DNAexonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 184.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairputative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 149.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 94.7)
    Genetic information processingTranscriptionRNA processingribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 45.4)
  • TheSEED:  
    DNA-replication DNA polymerase III polC-type (EC 2.7.7.7) 
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha subunit (PF07733; HMM-score: 331.3)
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 182.2)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 149.3)
    DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 63.1)
    no clan definedDNA_pol3_a_NI; DNA polymerase III polC-type N-terminus I (PF14480; HMM-score: 59.8)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 37.9)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 36.4)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.6)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 15.4)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 11.2)
    RNase_H (CL0219) CAF1; CAF1 family ribonuclease (PF04857; HMM-score: 5.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 26
    • Cmax: 0.112
    • Cmax_pos: 36
    • Smax: 0.17
    • Smax_pos: 22
    • Smean: 0.096
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEFHITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTALSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIFETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGKPIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFTRKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMIYGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEIIDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASFDMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAIYDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKNLFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIAKYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFEHDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELADRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIGNGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKTSEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEYQPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGCTGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLDILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVPEFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRDDIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFPKAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYSRYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPGLGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 42.51 h [4]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]