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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1286 [new locus tag: SACOL_RS06565 ]
  • pan locus tag?: SAUPAN003568000
  • symbol: SACOL1286
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1286 [new locus tag: SACOL_RS06565 ]
  • symbol: SACOL1286
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1299583..1299867
  • length: 285
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    ATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAA
    AAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGA
    AAGAAACAAGGCCGTGGCGCATATGTTTCTAAAGATGTTGCTATGGTTGAAAAAGCACAA
    CAAAAAGAAATTTTAGAAAAATATTTTAAAGCATCTAAAGAGCAATTGGATCCTGTTTAC
    AAAGAAATTATTAGATTAATTTATAGAGAAGAGATCCCAAAATGA
    60
    120
    180
    240
    285

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1286 [new locus tag: SACOL_RS06565 ]
  • symbol: SACOL1286
  • description: hypothetical protein
  • length: 94
  • theoretical pI: 10.3227
  • theoretical MW: 11040.1
  • GRAVY: -0.705319

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    RNA MetabolismTranscriptionTranscription factors bacterial COG2740: Predicted nucleic-acid-binding protein implicated in transcription termination 
    NusA-TFII Cluster COG2740: Predicted nucleic-acid-binding protein implicated in transcription termination 
  • PFAM:
    no clan definedDUF448; Protein of unknown function (DUF448) (PF04296; HMM-score: 84.4)
    ApoA-II; Apolipoprotein A-II (ApoA-II) (PF04711; HMM-score: 14.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.23
    • Ymax_pos: 11
    • Cmax: 0.107
    • Cmax_pos: 62
    • Smax: 0.719
    • Smax_pos: 1
    • Smean: 0.486
    • D: 0.33
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQGRGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 171 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 13.92 h [4]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  4. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]