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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1316 [new locus tag: SACOL_RS06710 ]
  • pan locus tag?: SAUPAN003607000
  • symbol: mutL
  • pan gene symbol?: mutL
  • synonym:
  • product: DNA mismatch repair protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1316 [new locus tag: SACOL_RS06710 ]
  • symbol: mutL
  • product: DNA mismatch repair protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1333086..1335095
  • length: 2010
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGAAAATTAAAGAACTCCAAACCTCATTAGCAAATAAAATCGCAGCAGGTGAAGTA
    GTTGAAAGACCGAGTTCTGTTGTGAAAGAACTGTTGGAAAATGCTATAGATGCAGGCGCT
    ACAGAAATAAGCATTGAAGTAGAGGAATCTGGCGTCCAATCTATTCGCGTAGTCGATAAT
    GGAAGCGGAATTGAAGCGGAAGACTTAGGATTAGTATTTCATAGACATGCGACTAGTAAA
    TTAGATCAAGATGAAGATTTATTTCATATTAGGACATTAGGATTCCGTGGTGAAGCACTA
    GCCAGTATTTCATCAGTTGCTAAAGTAACATTGAAGACTTGCACGGATAATGCTAATGGA
    AATGAAATATATGTAGAAAATGGTGAAATATTAAATCATAAGCCTGCAAAAGCGAAAAAA
    GGAACAGATATACTTGTAGAATCATTATTTTATAATACACCAGCACGTTTAAAATATATT
    AAAAGTTTATACACTGAACTAGGTAAAATAACAGATATTGTCAACAGAATGGCAATGAGC
    CATCCGGACATTCGAATAGCACTCATTTCAGATGGCAAAACAATGTTAAGTACAAATGGT
    TCAGGACGAACTAATGAAGTGATGGCAGAGATTTATGGGATGAAAGTTGCACGAGATTTA
    GTACATATATCTGGAGATACAAGTGATTATCACATTGAAGGTTTTGTTGCAAAGCCTGAA
    CATTCTAGAAGTAATAAGCACTATATTTCTATTTTTATTAATGGACGATACATTAAAAAC
    TTTATGCTAAATAAAGCGATTTTAGAAGGCTATCATACACTCTTAACAATAGGTAGGTTC
    CCGATTTGTTATATTAATATTGAAATGGATCCAATCTTAGTAGACGTAAATGTTCATCCA
    ACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAA
    ATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCA
    AAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAA
    AACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTT
    ATGGAAGAAAATCAAAACGATGAGATAGTTATAAAAGAAGATTCATATAATCCATTCGTA
    ACGAAAACATCTGAAAGTTTAATAGCTGATGATGAATCTTCTGGTTATAATAATACACGT
    GAAAAAGATGAAGACTACTTCAAAAAACAACAAGAAATTTTACAAGAAATGGATCAAACA
    TTTGATTCAAATGATGGTACAACTGTGCAAAATTATGAGAATAAAGCGTCTGATGATTAT
    TATGATGTAAACGATATTAAAGGAACAAAAAGTAAAGACCCTAAACGAAGAATTCCATAT
    ATGGAAATTGTTGGACAAGTACATGGAACGTATATTATTGCTCAAAATGAATTTGGCATG
    TACATGATTGACCAGCATGCAGCTCAAGAAAGAATAAAATATGAATATTTTCGAGATAAA
    ATAGGTGAGGTTACCAATGAAGTACAAGATTTATTAATCCCGTTAACATTTCATTTTTCA
    AAAGATGAACAATTAGTCATTGATCAATATAAAAATGAGCTTCAACAAGTAGGTATCATG
    TTAGAACATTTTGGTGGTCATGATTATATTGTAAGTAGCTATCCAGTTTGGTTCCCTAAA
    GATGAAGTAGAAGAAATTATTAAAGATATGATTGAGCTAATTTTGGAAGAGAAAAAAGTA
    GATATCAAAAAATTACGTGAAGATGTAGCAATCATGATGTCATGTAAAAAATCCATTAAA
    GCGAATCATTATTTACAAAAACATGAAATGTCTGATTTAATTGATCAATTAAGAGAAGCG
    GAAGATCCATTTACATGTCCACATGGTCGTCCAATAATCATTAATTTTTCAAAATACGAA
    TTAGAAAAATTATTTAAGCGTGTGATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2010

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1316 [new locus tag: SACOL_RS06710 ]
  • symbol: MutL
  • description: DNA mismatch repair protein
  • length: 669
  • theoretical pI: 5.05367
  • theoretical MW: 76854.5
  • GRAVY: -0.538715

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA mismatch repair protein MutL (TIGR00585; HMM-score: 338.9)
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 16.1)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 16.1)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 10.8)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial MutL-MutS system DNA mismatch repair protein MutL 
  • PFAM:
    no clan definedMutL_C; MutL C terminal dimerisation domain (PF08676; HMM-score: 157.2)
    S5 (CL0329) DNA_mis_repair; DNA mismatch repair protein, C-terminal domain (PF01119; HMM-score: 130.2)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 48.6)
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 35.2)
    FixH-like (CL0488) FixH; FixH (PF05751; HMM-score: 11.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 41
    • Cmax: 0.147
    • Cmax_pos: 41
    • Smax: 0.202
    • Smax_pos: 17
    • Smean: 0.127
    • D: 0.13
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEISIEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALASISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYIKSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVARDLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLTIGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKNNLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIKEDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQNYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAAQERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGGHDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHYLQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 60 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]