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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1420 [new locus tag: SACOL_RS07230 ]
  • pan locus tag?: SAUPAN003793000
  • symbol: SACOL1420
  • pan gene symbol?: phoU
  • synonym:
  • product: phosphate transport system protein PhoU

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1420 [new locus tag: SACOL_RS07230 ]
  • symbol: SACOL1420
  • product: phosphate transport system protein PhoU
  • replicon: chromosome
  • strand: -
  • coordinates: 1431554..1432195
  • length: 642
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAATAATTAGACAACGATATCAGGAGCAACTTGATGATTTAATAAAAGAATTACGT
    CGGTTAGGTGCAAATGTCTATGTGAGTATTGAAAATGGTATAAAATCATTAAGTATTGAC
    GATAGAGGCTTTGCACGACAAACAGTTAAAAACGATAAACATATCAATCAATTAAATTAT
    GATATTAATGAGCGAGTTATCATGTTAATTACAAAGCAACAGCCCATTGCGAGTGATTTG
    CGTATGATGATTTCTTCATTAAAAATCGCCTCCGATTTAGAAAGAATAGGAGATAATGCC
    TCGAGTATTGCCAATATTCGATTGCGTACAAAGATTACAGATGATTATGTGTTAACCCGT
    TTAAAGACAATGGGTAAATTAGCTATGTTAATGTTAAAGGACTTAGATCAAGCATTTAAA
    AAGAAAGATACCGTATTAATAAGAGAAATAATTGAGCGTGATGAAGATATCGATGACTTA
    TATAGTCATATTATTAACGCAACGTATCTTATTGATAACGATCCATTTGTCGCTGCACAA
    GCTCATTTAGCAGCAAGACATTTAGAACGTATTGGTGATCATATTATTAACATCGCTGAA
    AGTGTTTATTTTTATTTAACAGGTACACATTACGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1420 [new locus tag: SACOL_RS07230 ]
  • symbol: SACOL1420
  • description: phosphate transport system protein PhoU
  • length: 213
  • theoretical pI: 6.5234
  • theoretical MW: 24619.1
  • GRAVY: -0.284507

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 225.6)
    Signal transductionRegulatory functionsOtherphosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 225.6)
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryHigh affinity phosphate transporter and control of PHO regulon Phosphate transport system regulatory protein PhoU 
    Phosphorus Metabolism - no subcategoryPhosphate metabolism Phosphate transport system regulatory protein PhoU 
  • PFAM:
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 125)
    no clan definedBclx_interact; Bcl-x interacting, BH3 domain (PF08945; HMM-score: 10)
    Met_repress (CL0057) SeqA_N; SeqA protein N-terminal domain (PF17206; HMM-score: 8.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.108
    • Ymax_pos: 46
    • Cmax: 0.111
    • Cmax_pos: 46
    • Smax: 0.14
    • Smax_pos: 38
    • Smean: 0.089
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGFARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASSIANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDLYSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]