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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1428 [new locus tag: SACOL_RS07275 ]
  • pan locus tag?: SAUPAN003806000
  • symbol: lysC
  • pan gene symbol?: lysC
  • synonym:
  • product: aspartate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1428 [new locus tag: SACOL_RS07275 ]
  • symbol: lysC
  • product: aspartate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1440601..1441806
  • length: 1206
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGTAACAAGAAGTGTGTTGAAATTTGGCGGATCATCCGTCAGTGATTTTACAAAATTA
    AAAAGGATCGCTGAAATGTTAAAGGAGCGAGTCAATCAAGATGAACAATTAATTGTCGTT
    GTAAGTGCTATGGGTAACACAACAGATCAATTAATGACGAATGTATCAACCTTGACTAAA
    GCACCAAAACAACAAGAACTGGCATTATTATTGACAACCGGAGAGCAACAAACTGTATCT
    TATTTATCAATGGTATTAAATGATATCGGTATGAATGCCAAAGCAATGACTGGCTATCAA
    GCGGGTATTAAAACCATTGGCCATCATTTAAAAAGTAAAATTGCTCAAATTAATCCTCAA
    ACATTTGAACAAGCCTTTCAAGAAAACGATATTTTAGTAGTTGCTGGATTTCAAGGCATC
    AATGAACATCAGGAATTAACAACTTTAGGCAGAGGTGGTTCTGATACGACCGCTGTGGCA
    CTTGCTGTTAGTAATCAAATACCTTGTGAAATTTATACCGACGTTGATGGTGTGTATGCC
    ACTGACCCAAGACTTTTACCAAAGGCTAAACGACTAGACATCGTCTCATATGAAGAAATG
    ATGGAAATGAGCGCTTTAGGTGCTGGTGTACTTGAAACAAGAAGTGTTGAATTAGCTAAA
    AACTATAATATCCCTTTATATTTAGGAAAAACTTTATCGAACGTGAAAGGAACATGGATT
    ATGTCAAATGAAGAAATATTAGAGAAAAAAGCAGTTACTGGTGTGGCTTTGGATAAACAT
    ATGATGCATGTAACAATTAGTTATCCCCTACCTGACAATCAGCTACTTACCCAACTATTT
    ACGGAACTTGAAGAAGGTGCTGTAAATGTTGATATGATTTCACAAATCGTCAACTTGGAT
    GGGCTACAACTATCCTTCACGATTAAAGATAGTGATTTTCATCAAATTTCTATGATTCTT
    GAAACATTAAAGAATCAATATGAAGCATTAGCTTATAAAATCAATGAGCATTATGTCAAA
    ATTTCATTAATTGGCTCAGGCATGCGTGATATGTCAGGTGTGGCATCAAAAGCATTTTTG
    ACATTAATTGAAAATAATATACCTTTCTACCAAACAACAACATCTGAAATAAGTATTTCA
    TACGTCATTGATGATTTTAATGGGCAACAAGCGGTAGAAAAACTATATGACGCATTTAAC
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1428 [new locus tag: SACOL_RS07275 ]
  • symbol: LysC
  • description: aspartate kinase
  • length: 401
  • theoretical pI: 4.70763
  • theoretical MW: 44374.6
  • GRAVY: -0.0608479

Function[edit source | edit]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinaseATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyaspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 352.2)
    MetabolismAmino acid biosynthesisAspartate familyaspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 329.7)
    MetabolismAmino acid biosynthesisAspartate familyputative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 105.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsUMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsputative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 31.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiamine pyrophosphokinase (TIGR01378; EC 2.7.6.2; HMM-score: 16.2)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway Aspartokinase (EC 2.7.2.4) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Aspartokinase (EC 2.7.2.4) 
  • PFAM:
    no clan definedAA_kinase; Amino acid kinase family (PF00696; HMM-score: 147.8)
    ACT (CL0070) ACT_7; ACT domain (PF13840; HMM-score: 26.9)
    no clan definedTPK_catalytic; Thiamin pyrophosphokinase, catalytic domain (PF04263; HMM-score: 20.4)
    E-set (CL0159) Alpha_adaptinC2; Adaptin C-terminal domain (PF02883; HMM-score: 14.7)
    Reo_sigma (CL0326) Reo_sigmaC; Reovirus sigma C capsid protein (PF04582; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.183
    • Ymax_pos: 11
    • Cmax: 0.123
    • Cmax_pos: 61
    • Smax: 0.43
    • Smax_pos: 2
    • Smean: 0.325
    • D: 0.238
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVTRSVLKFGGSSVSDFTKLKRIAEMLKERVNQDEQLIVVVSAMGNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAGIKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVALAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVELAKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLLTQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKINEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAVEKLYDAFNI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]