From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1548 [new locus tag: SACOL_RS07885 ]
  • pan locus tag?: SAUPAN004037000
  • symbol: SACOL1548
  • pan gene symbol?: rnz
  • synonym:
  • product: AtsA/ElaC family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1548 [new locus tag: SACOL_RS07885 ]
  • symbol: SACOL1548
  • product: AtsA/ElaC family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1582934..1583854
  • length: 921
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAGTTACATTTTTTGGAACGAGTGCAGGTTTGCCTACAAAAGAGAGAAATACACAA
    GCAATCGCCTTAAATTTAGAACCATATTCCAATTCCATATGGCTTTTCGACGTTGGTGAA
    GGTACACAGCACCAAATTTTACATCATGCAATTAAATTAGGAAAAGTGACACATATATTT
    ATTACTCATATGCATGGCGATCATATTTTTGGTTTGCCAGGATTACTTTCTAGTCGTTCT
    TTTCAGGGCGGTGAACAGAAGCCGCTTACATTGGTTGGACCAAAAGGAATTAAAGCATAT
    GTGGAAATGTCTATGAATTTATCAGAGTCACATTTAAACTATCCAATTACATATATTGAA
    ATCGATGATCATCTGACATATCATCATGATGGTTTTACTGTAGAGGCGCATTTACTTAAT
    CATGGTATTCCATCATATGGATATAGAGTAATGGCACCTGAAACTACAGGTACAATCAAT
    GTTGAAGCGTTAAAAAACATTGGTCTTGAACCAGGTCCTAAATATCAAGAAGTAAAATCT
    CATGATACATTTGAACATAATGGTCAAGTTTATCAATCAAAAGATTTTAGAGGTGAGTCC
    AAACAGGGTCCAGTTGTAGCAATTTTTGGTGATACAAAACCATGTTCTAATGAACGTGTG
    ATTTCTAGAGATGCTGATGTGATGGTACATGAAGCGACCTATATAGATGGTGAAAAGCAT
    TTAGCCAACAATTATCATCATAGCCATATTGAAGACGTATTTGCATTGATAAAAGAAGCC
    AATGTTAAACGTACGTTGATTACACATTTGAGCAATCGCTATAATACAGAAGATATAAAT
    GAAATTTATCAAACATTGATACAAAATGAAGATACACCTAATTTTAATTTTGTAAAAGAT
    TTTGATAGTTTTAAAATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1548 [new locus tag: SACOL_RS07885 ]
  • symbol: SACOL1548
  • description: AtsA/ElaC family protein
  • length: 306
  • theoretical pI: 6.22105
  • theoretical MW: 34584.5
  • GRAVY: -0.446078

Function[edit source | edit]

  • reaction:
    EC 3.1.26.11?  ExPASy
    Ribonuclease ZEndonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule
  • TIGRFAM:
    Genetic information processingTranscriptionRNA processingribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 338.2)
    Genetic information processingTranscriptionRNA processingribonuclease BN (TIGR02649; EC 3.1.-.-; HMM-score: 251.7)
    Genetic information processingTranscriptionRNA processingribonuclease Z, Thermotoga type (TIGR02650; EC 3.1.26.11; HMM-score: 56.4)
    Cellular processesCellular processesDetoxificationhydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 15.1)
    Unknown functionEnzymes of unknown specificityquinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 12.9)
    phosphonate metabolism protein PhnP (TIGR03307; HMM-score: 12.2)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationtRNA processing Ribonuclease Z (EC 3.1.26.11) 
  • PFAM:
    Metallo-HOrase (CL0381) Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 54.3)
    Lactamase_B_4; tRNase Z endonuclease (PF13691; HMM-score: 33)
    Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 31.7)
    no clan definedMBF2; Transcription activator MBF2 (PF15868; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 22
    • Cmax: 0.147
    • Cmax_pos: 22
    • Smax: 0.257
    • Smax_pos: 19
    • Smean: 0.134
    • D: 0.143
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQHQILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVEMSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTINVEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNERVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNTEDINEIYQTLIQNEDTPNFNFVKDFDSFKI

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 105 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL1548 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]