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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1555 [new locus tag: SACOL_RS07925 ]
  • pan locus tag?: SAUPAN004064000
  • symbol: SACOL1555
  • pan gene symbol?:
  • synonym:
  • product: M20/M25/M40 family peptidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1555 [new locus tag: SACOL_RS07925 ]
  • symbol: SACOL1555
  • product: M20/M25/M40 family peptidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1591772..1592905
  • length: 1134
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATTAATGAGCAAAGATTACTTAATACGTTTTTTGAACTTGTACAAATCGATTCTGAG
    ACAGGGAATGAATCAACAATCCAACCTATTTTAAAAGAAAAATTCATAGCATTAGGGTTA
    GATGTTAAAGAAGATGAAGCGGCTAAACATCCTAAATTAGGTGCCAATAATCTAGTGTGT
    ACAATGAATAGTACTATCGAAGAAGGCGAAGTACCTAAATTATATTTGACGAGCCATATG
    GATACTGTTGTACCCGCAATTAATGTAAAGCCAATTGTAAAAGATGACGGCTACATATAC
    TCTGATGGTACGACTATTCTAGGTGCAGATGATAAAGCAGGATTAGCAGCGATGCTTGAA
    GTTTTGCAAGTGATAAAGGAACAACAAATCCCACATGGACAAATTCAATTTGTGATTACT
    GTTGGAGAAGAATCAGGTTTAATTGGTGCTAAAGAATTAAATTCAGAGTTGTTGGACGCG
    GACTTTGGTTATGCTATTGATGCAAGTGCTGATGTCGGTACTACGGTTGTAGGTGCACCG
    ACGCAAATGTTAATTTCAGCTAAAATTTTTGGCAAAACGGCTCATGCAAGTACGCCAAAA
    GAGGGTGTTAGTGCTATTAATATTGCGGCGAAAGCAATTAGTCGAATGAAATTAGGACAA
    GTTGATGAAATAACTACTGCCAATATTGGTAAGTTTCATGGCGGTTCAGCTACGAATATT
    GTTGCAGATGAAGTCATTTTAGAAGCAGAGGCACGTTCTCACGATCCAGAAAGAATAAAA
    ACACAAGTCAAACATATGACTGATGTGTTTGAAACTACTGCATCAGAATTAGGTGGGAAA
    GCAGTAGTTACAGTGGAGCAAAGTTATCCAGGATTTAAAATTAATGATAATGAAGCGGTT
    GTTAAAATTGCACAAGAAAGTGCTCGTAATTTAGGATTATCTGCAAATACAATTATTTCT
    GGTGGCGGTTCAGACGGCAGTATTATTAATACATTTGGTATTCCTTCAGTCATTTTAGGA
    GTGGGTTATGAAAAAATCCATACAACAAATGAGAGAATGCCGATTAAATCATTGAACTTG
    CTTGCAAGTCAAGTGTTAGAAATAATCAAAATAGTTGCGCGACATTCGAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1134

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1555 [new locus tag: SACOL_RS07925 ]
  • symbol: SACOL1555
  • description: M20/M25/M40 family peptidase
  • length: 377
  • theoretical pI: 4.79982
  • theoretical MW: 40243.6
  • GRAVY: -0.00928382

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    peptidase T-like protein (TIGR01883; HMM-score: 402.7)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 114.4)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase T (TIGR01882; EC 3.4.11.-; HMM-score: 86.1)
    MetabolismAmino acid biosynthesisGlutamate familyacetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 84.7)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 78.1)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesamidohydrolase (TIGR01891; HMM-score: 77.8)
    MetabolismAmino acid biosynthesisAspartate familysuccinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 67.7)
    MetabolismAmino acid biosynthesisAspartate familysuccinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 57.5)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 38.1)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 38)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 26.2)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesdipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 25.7)
    hydrolase, peptidase M42 family (TIGR03106; HMM-score: 23.4)
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 21.2)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 12.6)
  • TheSEED:  
    Peptidase T (EC 3.4.11.4) 
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 86.7)
    Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 62.4)
    no clan definedM20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 49.5)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 43.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 49
    • Cmax: 0.113
    • Cmax_pos: 23
    • Smax: 0.199
    • Smax_pos: 47
    • Smean: 0.105
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKEDEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSDGTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDADFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKLGQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASELGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGIPSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 518 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]