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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1615 [new locus tag: SACOL_RS08235 ]
  • pan locus tag?: SAUPAN004139000
  • symbol: SACOL1615
  • pan gene symbol?: cshB
  • synonym:
  • product: DEAD/DEAH box helicase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1615 [new locus tag: SACOL_RS08235 ]
  • symbol: SACOL1615
  • product: DEAD/DEAH box helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 1645088..1646434
  • length: 1347
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCAAAACATCCATTCGAACAATTTAATCTAGAATCTAGTTTAATTGACGCTGTGAAA
    GACCTTAATTTTGAAAAACCAACTGAAATTCAGAATCGAATTATTCCAAGAATACTAAAG
    AGAACAAATTTAATTGGTCAATCTCAAACGGGTACAGGGAAATCTCATGCATTTTTATTA
    CCATTAATGCAGTTAATTGATAGTGAAATAAAAGAACCACAAGCAATCGTAGTTGCACCA
    ACAAGAGAACTTGCACAACAACTATACGATGCAGCGAACCATTTAAGCCAATTTAAAGCT
    GGTGTTTCAGTTAAAGTTTTTATTGGTGGTACAGATATAGAGAAAGATAGACAACGTTGT
    AATGCACAACCACAATTGATTATAGGCACCCCTACTAGAATTAATGACTTAGCTAAAACG
    GGACATTTACATGTGCACTTAGCATCATATTTAGTTATTGATGAAGCGGATCTTATGATT
    GACTTAGGATTAATTGAAGATGTAGATTACATTGCTGCAAGATTGGAAGATAATGCAAAT
    ATTGCGGTGTTTAGTGCTACAATCCCACAACAGTTACAACCATTTTTAAATAAATATTTA
    AGTCATCCAGAATATGTAGCTGTCGACAGTAAAAAACAAAATAAAAAGAACATCGAATTC
    TATTTAATACCTACTAAAGGTGCAGCTAAAGTTGAAAAGACTTTAAATTTAATTGATATA
    CTAAATCCATACTTATGTATTATTTTCTGTAATAGTAGAGATAATGCAAATGATTTAGCA
    CGTTCACTAAATGAAGCTGGTATTAAAGTTGGTATGATTCATGGTGGCTTAACGCCACGT
    GAACGTAAACAACAAATGAAACGTATACGTAATTTAGAATTCCAATACGTTATTGCCAGC
    GATTTAGCATCTCGTGGTATTGATATTGAAGGTGTTAGTCATGTCATCAATTTTGATGTG
    CCAAATGATATTGACTTCTTTACGCATAGAGTCGGACGAACTGGTCGTGGGAATTATAAA
    GGTGTAGCAATTACGCTTTATAGTCCTGATGAAGAACACAATATTTCATTAATAGAAGAT
    CGCGGTTTTGTATTCAATACTGTTGATATTAAAGATGGTGAGTTAAAAGAAGTTAAAGCG
    CACAATCAGCGTCAAGCAAGAATGCGCAAAGATGACCATTTAACTAATCAAGTGAAGAAC
    AAAGTTCGAAGTAAAATTAAAAACAAAGTTAAACCAGGTTATAAGAAGAAATTTAAACAA
    GAAGTTGAAAAAATGAAACGTCAAGAGCGTAAGCAATTTAGTAAGCAGCAAAATAGACAA
    AAACGTAAGCAAAACAAAAAAGGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1615 [new locus tag: SACOL_RS08235 ]
  • symbol: SACOL1615
  • description: DEAD/DEAH box helicase
  • length: 448
  • theoretical pI: 10.1086
  • theoretical MW: 51080.3
  • GRAVY: -0.565179

Function[edit source | edit]

  • reaction:
    EC 3.6.4.13?  ExPASy
    RNA helicaseATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 80.2)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 68.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 67.2)
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 47.3)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 40.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 30)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 19.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 16.3)
    CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 13.9)
    probable extracellular repeat, HAF family (TIGR02913; HMM-score: 12.1)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationATP-dependent RNA helicases, bacterial DEAD-box ATP-dependent RNA helicase CshB (EC 3.6.4.13) 
  • PFAM:
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 131.7)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 98.1)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 27)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 58
    • Cmax: 0.112
    • Cmax_pos: 58
    • Smax: 0.143
    • Smax_pos: 53
    • Smean: 0.085
    • D: 0.101
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNLIGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGVSVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMIDLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNIEFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGGLTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRTGRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDDHLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 404 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 87.97 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]