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NCBI: 10-JUN-2013

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1709 [new locus tag: SACOL_RS08730 ]
  • pan locus tag?: SAUPAN004274000
  • symbol: folC
  • pan gene symbol?: folC
  • synonym:
  • product: folylpolyglutamate synthase/dihydrofolate synthase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1709 [new locus tag: SACOL_RS08730 ]
  • symbol: folC
  • product: folylpolyglutamate synthase/dihydrofolate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1738352..1739623
  • length: 1272
  • essential: unknown other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATTACCTAGAGAGCTTGTATTGGATACACGAAAGAACTAAATTTGGCATCAAACCA
    GGTGTTAAACGTATGGAATGGATGCTAGCACAATTTAATAATCCTCAAAATAACATTAAG
    GGTATTCATGTAGGTGGCACAAATGGTAAAGGCTCTACAGTTGCTTACCTTAGAACAGCT
    TTAGTTGAAAATGGTTATGAAGTAGGTACATTTACGTCGCCGTTTATTGAAACATTTAAT
    GAACGAATTAGTCTAAATGGTGTGCCAATATCAAATGACGCTATTGTAGAATTAGTATCA
    CGTATTAAACCAGTAAGTGAAATGATGGAACGTGAAACAGATTTAGGTGTTGCAACTGAA
    TTCGAAATAATCACAGCGATGATGTTTTTATATTTTGGTGAAATACATCCTGTTGATTTT
    GTCATTGTTGAGGCTGGATTGGGTATAAAGAACGATTCGACAAATGTCTTTACACCGGTT
    TTATCAATCTTAACTAGTATCGGTCTAGACCATACAGATATTTTAGGTGGTACTTATCTA
    GATATTGCTAGGGATAAAGGCGCGATTATAAAGCCTAACGTTCCAGTGATATATGCTGTT
    AAAAATGAAGATGCATTAAAATATGTTCGTGAACGCGCAATTGAACAACATGCAAAGCCA
    ATTGAATTAGATAGAGAAATTGTTGTTGTATCGCAAAATGATGAATTTACTTACCGTTAT
    AAAGATTATGAATTAGAAACAATCATTTTAAGCATGTTAGGTGAACATCAGAAACAAAAT
    GCTGCATTAGCCATAACAGCTCTTATTGAATTAAATGAACAAGGATTAATTGAATTAGAT
    TTCAATAAGATGATAGACGGTATTGAATCAGTTCGTTGGACTGGACGTATTGAGCAGGTG
    CATGACAAACCTTTAATCATTTTGGATGGCGCACATAATTCAGAGAGTATAGATGCTCTA
    ATTGATACAATTAAACAGTACCATGATAAAGAAAAAGTAGATATTTTGTTCTCAGCAATA
    AACGGAAAACCGATTAACGAGATGGTCAAACATTTAAGTTTAATTGCGCATACGTTTTAT
    GCAACTGAATTTGATTTTCCGAAAGCGTTACGCAAAGAAGAAATTGTAGGTAGTATTGAA
    AATGATGAAATACAATTAGTAGATGACTACGTTGAATTTATAAAAAATTATCAAGGTGAT
    ACATTAGTAATTACCGGTAGTCTGTATTTCATAAGTGAAGTTAAATCAAAATTAGACTTT
    GAAGCATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1272

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: SACOL1709 [new locus tag: SACOL_RS08730 ]
  • symbol: FolC
  • description: folylpolyglutamate synthase/dihydrofolate synthase
  • length: 423
  • theoretical pI: 4.55771
  • theoretical MW: 47856.3
  • GRAVY: -0.0959811

⊟Function[edit source | edit]

  • reaction:
    EC 6.3.2.17?  ExPASy
    Tetrahydrofolate synthaseATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidbifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 376.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 55)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 41.2)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 35.7)
    Cell structureCell envelopeOtherpoly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 19.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 15.1)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis Dihydrofolate synthase (EC 6.3.2.12) 
    Folate and pterinesFolate Biosynthesis Folylpolyglutamate synthase (EC 6.3.2.17) 
    Virulence, Disease and DefenseBacteriocins, ribosomally synthesized antibacterial peptidesColicin V and Bacteriocin Production Cluster Dihydrofolate synthase (EC 6.3.2.12) 
    Bacteriocins, ribosomally synthesized antibacterial peptidesColicin V and Bacteriocin Production Cluster Folylpolyglutamate synthase (EC 6.3.2.17) 
  • PFAM:
    no clan definedMur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 42.8)
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 27.5)
    ACT (CL0070) ALS_ss_C; Small subunit of acetolactate synthase (PF10369; HMM-score: 13.8)
    P-loop_NTPase (CL0023) AAA_14; AAA domain (PF13173; HMM-score: 13.5)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 42
    • Cmax: 0.115
    • Cmax_pos: 25
    • Smax: 0.194
    • Smax_pos: 37
    • Smean: 0.135
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPVLSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDFEAL

⊟Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 15 [4]

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

⊟Regulation[edit source | edit]

  • sigma factor:
  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit source | edit]

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