NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL1711 [new locus tag: SACOL_RS08740 ]
• pan locus tag^?: SAUPAN004277000
• symbol: SACOL1711
• pan gene symbol^?: tag
• synonym:
• product: DNA-3-methyladenine glycosylase

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL1711 [new locus tag: SACOL_RS08740 ]
• symbol: SACOL1711
• product: DNA-3-methyladenine glycosylase
• replicon: chromosome
• strand: +
• coordinates: 1742669..1743229
• length: 561
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3238360 NCBI
• RefSeq: YP_186550 NCBI
• MicrobesOnline: 913159 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAATGAATGCGCATTTGGTACTAAAGATCCAGTCTACTTAGACTATCATGATCATGTA
  TGGGGACAACCGCTCTATGATAGCAAGGCATTGTTTAAACTTTTAGCATTAGAATCACAA
  CATGCTGGGCTATCTTGGTTAACTATTTTAAAAAAGAAAGAAGCCTCTGAAGAAGCATTT
  TATGATTTCGAACCAGAAAAGGTAGCACAAATGACCGCTCAAGATATCGACCGCTTAATG
  ACTTTTCCAAATATCGTTCATCATCGTAAAAAATTAGAAGCAATTGTTAATCAAGCTCAA
  GGGTATTTAAAAATTGAACAAGCATATGGTAGTTTTAGTAAATTTTTATGGTCATATGTA
  AATGGTAAGCCTAAAGATTTGCAGTATGAACATGCTTCTGATCGTATCACAGTTGATGAT
  ACTGCAACACAACTATCTAAAGATTTAAAACAATACGGGTTTAAATTTTTAGGTCCAGTA
  ACAGTATTTTCGTTTTTAGAAGCAGCCGGTTTATATGATGCACATTTACAAGATTGTCCA
  TCAAAGCCTAAACATAATTAA
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  561
  

⊟Protein

⊟General

• locus tag: SACOL1711 [new locus tag: SACOL_RS08740 ]
• symbol: SACOL1711
• description: DNA-3-methyladenine glycosylase
• length: 186
• theoretical pI: 6.51001
• theoretical MW: 21340
• GRAVY: -0.474194

⊟Function

• reaction:
  EC 3.2.2.-?  ExPASy
• TIGRFAM:
  Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 186.1)
• TheSEED:  
  DNA MetabolismDNA repairDNA Repair Base Excision DNA-3-methyladenine glycosylase (EC 3.2.2.20) 
• PFAM:
  no clan definedAdenine_glyco; Methyladenine glycosylase (PF03352; HMM-score: 210.5)
  DUF3326; Protein of unknown function (DUF3326) (PF11805; HMM-score: 11)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.126
  • Ymax_pos: 56
  • Cmax: 0.127
  • Cmax_pos: 56
  • Smax: 0.203
  • Smax_pos: 41
  • Smean: 0.08
  • D: 0.108
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57650537 NCBI
• RefSeq: YP_186550 NCBI
• UniProt: A0A0H2WW92 UniProt

⊟Protein sequence

• MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGLSWLTILKKKEASEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGYLKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPVTVFSFLEAAGLYDAHLQDCPSKPKHN

⊟Experimental data

• experimental localization: Cytoplasmic ^[1]

⊟Expression & Regulation

⊟Operon

• SACOL1711 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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