NCBI: 10-JUN-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1711 [new locus tag: SACOL_RS08740 ]
- pan locus tag?: SAUPAN004277000
- symbol: SACOL1711
- pan gene symbol?: tag
- synonym:
- product: DNA-3-methyladenine glycosylase
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SACOL1711 [new locus tag: SACOL_RS08740 ]
- symbol: SACOL1711
- product: DNA-3-methyladenine glycosylase
- replicon: chromosome
- strand: +
- coordinates: 1742669..1743229
- length: 561
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 3238360 NCBI
- RefSeq: YP_186550 NCBI
- MicrobesOnline: 913159 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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541ATGAATGAATGCGCATTTGGTACTAAAGATCCAGTCTACTTAGACTATCATGATCATGTA
TGGGGACAACCGCTCTATGATAGCAAGGCATTGTTTAAACTTTTAGCATTAGAATCACAA
CATGCTGGGCTATCTTGGTTAACTATTTTAAAAAAGAAAGAAGCCTCTGAAGAAGCATTT
TATGATTTCGAACCAGAAAAGGTAGCACAAATGACCGCTCAAGATATCGACCGCTTAATG
ACTTTTCCAAATATCGTTCATCATCGTAAAAAATTAGAAGCAATTGTTAATCAAGCTCAA
GGGTATTTAAAAATTGAACAAGCATATGGTAGTTTTAGTAAATTTTTATGGTCATATGTA
AATGGTAAGCCTAAAGATTTGCAGTATGAACATGCTTCTGATCGTATCACAGTTGATGAT
ACTGCAACACAACTATCTAAAGATTTAAAACAATACGGGTTTAAATTTTTAGGTCCAGTA
ACAGTATTTTCGTTTTTAGAAGCAGCCGGTTTATATGATGCACATTTACAAGATTGTCCA
TCAAAGCCTAAACATAATTAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SACOL1711 [new locus tag: SACOL_RS08740 ]
- symbol: SACOL1711
- description: DNA-3-methyladenine glycosylase
- length: 186
- theoretical pI: 6.51001
- theoretical MW: 21340
- GRAVY: -0.474194
⊟Function[edit source | edit]
- reaction: EC 3.2.2.-? ExPASy
- TIGRFAM: DNA metabolismDNA replication, recombination, and repairDNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 186.1)
- TheSEED:
- PFAM: no clan definedAdenine_glyco; Methyladenine glycosylase (PF03352; HMM-score: 210.5)DUF3326; Protein of unknown function (DUF3326) (PF11805; HMM-score: 11)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.126
- Ymax_pos: 56
- Cmax: 0.127
- Cmax_pos: 56
- Smax: 0.203
- Smax_pos: 41
- Smean: 0.08
- D: 0.108
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGLSWLTILKKKEASEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGYLKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPVTVFSFLEAAGLYDAHLQDCPSKPKHN
⊟Experimental data[edit source | edit]
- experimental localization: Cytoplasmic [1]
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- SACOL1711 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
- ↑
Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int. J. Med. Microbiol.: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit source | edit]