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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1823 [new locus tag: SACOL_RS09345 ]
  • pan locus tag?: SAUPAN004464000
  • symbol: arsB
  • pan gene symbol?: arsB
  • synonym:
  • product: arsenical pump membrane protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1823 [new locus tag: SACOL_RS09345 ]
  • symbol: arsB
  • product: arsenical pump membrane protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1878780..1880072
  • length: 1293
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATGACAACTTTAGCGACACTCATTTTTCTAGTAACTTTATTATTTGTATTATGGCAA
    CCTAAAGGCCTAGATATTGGCATTACCGCACTAACTGGTGCCTTTATTGCTGTTATTACT
    GGTGTTGTAAGTTTTTCCGATGTTTTCGAAGTAACAGGTATTGTTTGGAATGCTACTTTG
    ACTTTTGTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGG
    TCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATC
    ATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTA
    ACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCT
    TTTATTATTGCGAGTGGTTTTATAGCTGACACAACTTCTTTACCTTTAATCGTGAGCAAT
    CTAGTGAATATTATATCTGCTGATTATTTTCATGTAGGATTCGTTCGATATTTTAGTAGA
    ATGATTATACCTAATTTATTCTCGCTTTTAGCAAGTATTATAGTATTGTGGTTATATTTT
    AGAAAAGCGATACCTAAAACGTTTGATGATAATAATATAAAGCATCCTAAAGATGCCATT
    AATGATTTAAAGCTATTTAAAATTTCATGGATTGTTCTAGTTATATTACTTTTCGGCTAT
    CTAATCAGTGAATTTACTAAAATTCCGGTATCAATTTTCACTGGAATCATTGCTTTTATT
    TTTCTAATGTTGGCTCGTAAATCAAATGCTGTAAATATTAAGCAAGTCATTAAGGGCGCA
    CCTTGGAATATAGTATTATTTTCAATTGGTATGTATATCGTCGTATTCGGCTTAAGAAAT
    GCTGGCATTACTTTAATATTGGCTAAAATATTAGAATATATTTCCAATTACGGTCTATTT
    AGCACTATTTTGGGAATGGGCTTCATTTCAGCGTTTTTATCATCAATAATGAATAATATG
    CCTACAGTTTTAATAGATGCGATTGCTATTGGTCAATCAAATGTCCATGGCATGTTAAAA
    GAAGGCCTAATTTATGCGAATGTTATCGGTTCTGATTTAGGTCCAAAAATTACACCGATA
    GGCTCTTTAGCTACATTACTGTGGTTACACGTCTTAACACAAAAAGATGTTAAGATTTCT
    TGGGGCACATACTTTAAAACTGGTATCATCATTACAATTCCAGTACTATTTATAACCCTC
    ATAGGGTTGTATCTAACACTTATCATATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1823 [new locus tag: SACOL_RS09345 ]
  • symbol: ArsB
  • description: arsenical pump membrane protein
  • length: 430
  • theoretical pI: 10.0887
  • theoretical MW: 47271.7
  • GRAVY: 1.09023

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesDetoxificationarsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 706.2)
    MetabolismTransport and binding proteinsOtherarsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 706.2)
  • TheSEED:  
    Virulence, Disease and DefenseResistance to antibiotics and toxic compoundsArsenic resistance Arsenic efflux pump protein 
  • PFAM:
    IT (CL0182) ArsB; Arsenical pump membrane protein (PF02040; HMM-score: 629.2)
    CitMHS; Citrate transporter (PF03600; HMM-score: 90.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.207
    • Ymax_pos: 11
    • Cmax: 0.143
    • Cmax_pos: 47
    • Smax: 0.587
    • Smax_pos: 1
    • Smean: 0.401
    • D: 0.283
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVSFSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIILLGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSNLVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKDAINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQVIKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLSSIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVLTQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF

Experimental data[edit source | edit]

  • experimental localization: Integral membrane [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: ArsR
    ArsR (TF) important in Arsenic resistance  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]