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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1844 [new locus tag: SACOL_RS09460 ]
  • pan locus tag?: SAUPAN004511000
  • symbol: SACOL1844
  • pan gene symbol?: menE
  • synonym:
  • product: O-succinylbenzoic acid--CoA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1844 [new locus tag: SACOL_RS09460 ]
  • symbol: SACOL1844
  • product: O-succinylbenzoic acid--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1898269..1899747
  • length: 1479
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGACTTTTGGTTATATAAACAAGCACAACAAAATGGACATCATATTGCGATAACAGAC
    GGTCAAGAATCTTATACTTATCAAAATTTATACTGTGAAGCGAGTCTATTGGCTAAAAGA
    CTCAAGGCTTATCAACAATCTCGTGTTGGGCTATACATAGATAATTCGATTCAATCGATC
    ATTTTAATACATGCATGTTGGTTGGCAAATATTGAAATTGCGATGATTAATACAAGGTTG
    ACACCTAATGAGATGACGAATCAGATGAAGTCAATCGATGTACAATTGATTTTTTGTACC
    TTGCCACTGGAATTACGAGGGTTTCAAATTGTATCGCTGGATGATATTGAATTCGCTGGA
    AGGGATATTACAACGAACAGTTTGTTGGACAACACAATGGGTATCCAATATGAAACATCG
    AATGAAACTGTGGTGCCGAAAGAGTCGCCGTCCAACATATTAAATACATCATTTAATTTA
    GATGACATTGCATCGATTATGTTTACATCAGGGACAACGGGCCCTCAAAAAGCGGTGCCA
    CAAACGTTTCGTAATCATTATGCCAGTGCAATCGGATGTAAAGAGAGCTTGGGATTTGAT
    CGTGACACTAATTGGCTATCTGTCTTGCCGATTTATCATATTTCGGGTCTCAGTGTACTT
    TTAAGAGCTGTTATTGAAGGTTTTACTGTGCGCATTGTTGATAAATTCAATGCCGAACAA
    ATTTTAACGATGATTAAAAATGAACGAATCACGCACATTTCGCTTGTGCCACAAACTTTA
    AATTGGCTTATGCAACAAGGTTTACATGAACCTTATAATTTACAAAAAATATTACTCGGC
    GGTGCTAAATTATCTGCCACTATGATAGAGACGGCATTACAATATAACCTGCCAATTTAT
    AATTCATTTGGTATGACTGAGACATGTTCGCAATTTTTAACAGCAACACCGGAAATGTTG
    CATGCACGTCCTGACACTGTAGGGATGCCAAGTGCCAATGTTGACGTAAAAATTAAAAAT
    CCTAATAAAGAAGGTCATGGAGAATTAATGATTAAAGGCGCCAATGTGATGAATGTATAT
    TTGTATCCAACAGATTTAACGGGTACGTTTGAAAATGGTTATTTTAATACGGGTGACATT
    GCTGAAATAGATCATGAAGGTTATGTCATGATTTATGACCGACGTAAAGATTTAATTATT
    AGTGGCGGTGAAAATATTTATCCATATCAAATTGAAACGGTAGCGAAGCAATTTCCAGGT
    ATCAGTGACGCAGTATGTGTAGGGCATCCTGATGATACTTGGGGCCAAGTGCCTAAATTA
    TATTTTGTCAGTGAAAGTGACATTTCAAAGGCACAATTGATTGCATATTTATCACAACAT
    TTAGCGAAATATAAAGTACCGAAACACTTTGAAAAGGTTGATACTTTACCTTATACATCA
    ACAGGTAAATTACAAAGAAATAAGTTGTATAGAGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1844 [new locus tag: SACOL_RS09460 ]
  • symbol: SACOL1844
  • description: O-succinylbenzoic acid--CoA ligase
  • length: 492
  • theoretical pI: 5.90461
  • theoretical MW: 55414.9
  • GRAVY: -0.192276

Function[edit source | edit]

  • reaction:
    EC 6.2.1.26?  ExPASy
    o-succinylbenzoate--CoA ligaseATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneO-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 663.8)
    MetabolismTransport and binding proteinsCations and iron carrying compounds(2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 148.4)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 147.2)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 142.1)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 139.3)
    amino acid adenylation domain (TIGR01733; HMM-score: 117.5)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 91.1)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 79.8)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 58.6)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 30.2)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 16.9)
    MetabolismCentral intermediary metabolismOtheracetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 14.9)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsQuinone cofactorsMenaquinone and Phylloquinone Biosynthesis O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26) 
    Quinone cofactorsMenaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26) 
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 259.4)
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 50.1)
    no clan definedDUF4176; Domain of unknown function (DUF4176) (PF13780; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 68
    • Cmax: 0.108
    • Cmax_pos: 66
    • Smax: 0.139
    • Smax_pos: 42
    • Smean: 0.075
    • D: 0.092
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAYQQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLPLELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNLDDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLSVLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQKILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANVDVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIYDRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISKAQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]