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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1951 [new locus tag: SACOL_RS10205 ]
  • pan locus tag?: SAUPAN004914000
  • symbol: SACOL1951
  • pan gene symbol?: murT
  • synonym:
  • product: Mur ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1951 [new locus tag: SACOL_RS10205 ]
  • symbol: SACOL1951
  • product: Mur ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 2011993..2013306
  • length: 1314
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGACAGTGGACGGCAATCCATCTAGCGAAATTGGCGCGTAAAGCAAGTAGAGCAGTA
    GGTAAAAGAGGAACAGATTTACCTGGACAAATCGCTAGAAAAGTGGATACAGATATATTA
    AGAAAATTAGCAGAGCAAGTTGATGATATTGTATTTATCAGTGGAACAAATGGTAAAACA
    ACGACTTCAAACTTAATTGGACATACTTTAAAAGCAAATAATATTCAAATTATACACAAT
    AATGAAGGTGCTAATATGGCTGCAGGTATAACTTCTGCATTCATCATGCAATCAACACCT
    AAGACTAAAATTGCGGTAATCGAAATTGATGAAGGTTCGATTCCACGTGTGTTAAAAGAA
    GTTACACCTTCAATGATGGTATTTACTAATTTCTTTAGAGATCAAATGGATCGCTTCGGT
    GAAATTGATATTATGGTTAATAACATTGCAGAGACAATTAGTAATAAAGGCATCAAATTA
    TTGCTAAATGCTGATGATCCATTTGTGAGTCGTTTGAAAATCGCAAGTGATACGATTGTG
    TACTATGGTATGAAAGCACATGCCCATGAATTTGAACAAAGTACGATGAATGAAAGTAGA
    TATTGTCCAAACTGTGGTCGCTTATTGCAATACGATTATATTCATTATAATCAAATTGGT
    CATTATCACTGTCAGTGTGGTTTCAAACGAGAGCAAGCAAAATATGAAATATCAAGTTTT
    GATGTGGCACCGTTTTTATATTTAAATATCAATGATGAAAAATATGATATGAAAATTGCA
    GGTGACTTTAACGCTTATAACGCGTTAGCAGCATATACTGTTTTAAGAGAGCTAGGGTTA
    AATGAACAAACAATTAAAAATGGCTTTGAAACGTATACATCAGACAATGGTCGTATGCAG
    TACTTTAAAAAAGAACGAAAAGAAGCGATGATCAATTTAGCTAAAAATCCTGCAGGAATG
    AATGCAAGTTTATCAGTTGGTGAACAATTAGAAGGCGAAAAAGTGTATGTTATTTCGCTA
    AATGATAACGCTGCAGATGGTCGAGATACTTCATGGATTTATGATGCAGATTTTGAAAAA
    TTATCTAAGCAACAAATTGAAGCTATCATCGTGACAGGTACACGAGCAGAAGAACTTCAA
    TTGCGATTGAAGTTAGCAGAGGTTGAAGTACCAATTATAGTTGAGCGTGATATTTATAAA
    GCAACGGCAAAGACTATGGATTATAAAGGTTTCACAGTTGCAATACCAAACTATACATCA
    TTAGCGCCTATGCTTGAACAATTAAACCGTTCGTTTGAAGGAGGTCAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1951 [new locus tag: SACOL_RS10205 ]
  • symbol: SACOL1951
  • description: Mur ligase
  • length: 437
  • theoretical pI: 6.52509
  • theoretical MW: 49205.7
  • GRAVY: -0.349428

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 64.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 61.2)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 58.3)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 46.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 45.8)
    Cell structureCell envelopeOtherpoly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 23.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidbifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 22)
    HprK-related kinase A (TIGR04352; HMM-score: 16.9)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 14.1)
  • TheSEED:  
    proposed amino acid ligase found clustered with an amidotransferase 
  • PFAM:
    no clan definedDUF1727; Domain of unknown function (DUF1727) (PF08353; HMM-score: 101.6)
    Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 68.6)
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 16.6)
    RING (CL0229) zf-C3H2C3; Zinc-finger (PF17122; HMM-score: 13.5)
    Zn_Beta_Ribbon (CL0167) OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 12.8)
    TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 12.7)
    zf-RING_7; C4-type zinc ribbon domain (PF02591; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.221
    • Ymax_pos: 16
    • Cmax: 0.11
    • Cmax_pos: 19
    • Smax: 0.536
    • Smax_pos: 9
    • Smean: 0.471
    • D: 0.318
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 96 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 22.74 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog.: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)