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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1955 [new locus tag: SACOL_RS10225 ]
  • pan locus tag?: SAUPAN004919000
  • symbol: dinP
  • pan gene symbol?: dinP
  • synonym:
  • product: DNA polymerase IV

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1955 [new locus tag: SACOL_RS10225 ]
  • symbol: dinP
  • product: DNA polymerase IV
  • replicon: chromosome
  • strand: -
  • coordinates: 2015109..2016179
  • length: 1071
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    TTGACTGAGAGACGAATTATTCATATAGATATGGACTATTTTTTTGCACAAGTCGAAATG
    AGAGATAACCCCAAATTGAAAGGGAAACCAGTCATTGTTGGTGGTAAAGCAAGCAGTAGG
    GGTGTTGTCTCTACGGCATCCTATGAAGCACGAAAATACGGCGTCCATTCAGCAATGCCT
    ATGTCACAAGCGCATAAATTATGTCCAAATGGATACTTTGTCACAAGTAATTTTGGTGCT
    TACCGAGAAACATCTGCGCAGATTATGTCTATTTTCCGAAGTTATACAGATAAGGTAGAA
    CCGATGTCATTGGATGAAGCGTATTTAGATATTACAGAATTAGTGAGACCAGACCTTCCT
    GCTTCGAAAATTGCTCAGTATATTAGAAAAGATATTCTAGAGCAGACACATTTAACAGCA
    TCTGCAGGTGTTTCTTATAACAAATTTTTAGCTAAATTAGCGAGTGGTATGAATAAACCT
    GATGGTATGACTGTGATTGATTATCAAAATGTCCATGATATTTTGATGACATTGGATATT
    GGAGATTTTCCAGGCGTAGGTAAAGCTTCCAAAAAAGTAATGCATGATAATGGTATTTTT
    AACGGTAGAGATTTATATGAGAAAACGGAATTTGAATTAATACGTTTGTTTGGAAAAAGA
    GGTCGGGGTTTATATAACAAGGCACGCGGTATTGACCATAGTGAAGTGAAATCATCAAGA
    GTAAGAAAATCAGTAGGGACTGAACGCACATTTGCAACAGACGTGAATGATGATGAAGAG
    ATTTTAAGAAAAGTATGGGAATTGTCAGGTAAAACAGCTGAACGTCTAAATAAATTACAG
    AAGTCAGCTAAAACTGTAACGGTTAAAATTAAAACTTATCAATTTGAAACGCTATCTAAA
    CAGATGAGTTTAAGAGATTCGGTTAGTTCTGAAGAAGATATTTATAATATTGCATATTTA
    CTTTATAACGATTTAAAAGACCCTGATGTACCAATTCGACTTATTGGTGTCACTGTAGGT
    AATTTAGAACAATCAACTTATAAAAATATGACGATATATGACTTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1955 [new locus tag: SACOL_RS10225 ]
  • symbol: DinP
  • description: DNA polymerase IV
  • length: 356
  • theoretical pI: 9.28925
  • theoretical MW: 40314.8
  • GRAVY: -0.433427

Function[edit source | edit]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymeraseDeoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial DNA polymerase IV (EC 2.7.7.7) 
  • PFAM:
    no clan definedIMS; impB/mucB/samB family (PF00817; HMM-score: 182.9)
    IMS_C; impB/mucB/samB family C-terminal domain (PF11799; HMM-score: 75.3)
    HHH (CL0198) IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 28.6)
    no clan definedDUF3219; Protein of unknown function (DUF3219) (PF11514; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 36
    • Cmax: 0.124
    • Cmax_pos: 50
    • Smax: 0.25
    • Smax_pos: 30
    • Smean: 0.112
    • D: 0.127
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVSTASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPMSLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKPDGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFGKRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERLNKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRLIGVTVGNLEQSTYKNMTIYDFI

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]