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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1958 [new locus tag: SACOL_RS10240 ]
  • pan locus tag?: SAUPAN004922000
  • symbol: SACOL1958
  • pan gene symbol?: dgkB
  • synonym:
  • product: lipid kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1958 [new locus tag: SACOL_RS10240 ]
  • symbol: SACOL1958
  • product: lipid kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2018568..2019515
  • length: 948
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGGAAACGTGCTAGAATCATTTATAACCCGACATCAGGTAAAGAGCTATTTAAAAGA
    GAATTACCTGATGCCTTAATAAAATTAGAAAAAGCGGGATATGAAACGAGTGCATATGCA
    ACCGAGAAAATAGGTGATGCCACACTTGAAGCAGAAAGAGCTATGCATGAAAATTATGAT
    GTATTAATCGCTGCAGGTGGTGATGGAACATTAAATGAAGTAGTTAATGGTATCGCAGAA
    AAGCCTAATCGTCCTAAGCTAGGTGTCATTCCTATGGGTACTGTTAATGACTTTGGACGT
    GCATTGCATATACCTAATGACATCATGGGGGCACTTGATGTCATCATTGAAGGTCATTCT
    ACTAAAGTAGATATTGGTAAAATGAATAATCGATACTTTATTAATTTAGCTGCAGGCGGA
    CAATTGACGCAAGTCTCTTATGAAACACCGAGTAAATTGAAATCTATTGTTGGTCCATTT
    GCTTATTACATCAAAGGTTTCGAAATGTTACCTCAAATGAAAGCTGTAGATTTAAGAATT
    GAATATGATGGTAATGTTTTCCAAGGAGAAGCATTATTATTCTTTTTAGGTTTAACAAAT
    TCAATGGCAGGATTTGAAAAATTAGTGCCGGACGCTAAGTTAGATGACGGCTATTTTACG
    TTAATTATAGTTGAAAAATCAAACCTTGCAGAACTTGGTCATATTATGACTTTAGCTTCA
    AGAGGAGAACATACTAAGCATCCTAAAGTTATTTATGAAAAAGCAAAGGCAATTAATATT
    TCATCATTCACAGACTTACAACTAAATGTAGATGGAGAATATGGTGGTAAATTACCAGCG
    AATTTCTTAAATCTAGAACGTCATATAGATGTCTTTGCACCTAATGATATTGTAAATGAA
    GAGTTAATAAATAATGATCATGTTGATGACAATTTAATCGAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    948

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1958 [new locus tag: SACOL_RS10240 ]
  • symbol: SACOL1958
  • description: lipid kinase
  • length: 315
  • theoretical pI: 4.92012
  • theoretical MW: 34886.6
  • GRAVY: -0.214921

Function[edit source | edit]

  • reaction:
    EC 2.7.1.107?  ExPASy
    Diacylglycerol kinase (ATP)ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificitylipid kinase, YegS/Rv2252/BmrU family (TIGR00147; EC 2.7.1.-; HMM-score: 307.9)
    Unknown functionEnzymes of unknown specificitylipid kinase YegS (TIGR03702; EC 2.7.1.-; HMM-score: 171.9)
  • TheSEED:  
    Diacylglycerol kinase, Bacillus type (EC 2.7.1.107) 
  • PFAM:
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 117)
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 21.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 11
    • Cmax: 0.145
    • Cmax_pos: 41
    • Smax: 0.311
    • Smax_pos: 4
    • Smean: 0.25
    • D: 0.193
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPFAYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 116 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]