From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2073 [new locus tag: SACOL_RS10845 ]
  • pan locus tag?: SAUPAN005355000
  • symbol: murF
  • pan gene symbol?: murF
  • synonym:
  • product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2073 [new locus tag: SACOL_RS10845 ]
  • symbol: murF
  • product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 2139480..2140838
  • length: 1359
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATTAATGTTACATTAAAGCAAATTCAATCATGGATTCCTTGTGAAATTGAAGATCAA
    TTTTTAAATCAAGAGATAAATGGAGTCACAATTGATTCACGAGCAATTTCTAAAAATATG
    TTATTTATACCATTTAAAGGTGAAAATGTTGACGGTCATCGCTTTGTCTCTAAAGCATTA
    CAAGATGGTGCTGGGGCTGCTTTTTATCAAAGAGGGACACCTATAGATGAAAATGTAAGC
    GGGCCTATTATATGGGTTGAAGACACATTAACGGCATTACAACAATTGGCACAAGCTTAC
    TTGAGACATGTAAACCCTAAAGTAATTGCCGTCACAGGGTCTAATGGTAAAACAACGACT
    AAAGATATGATTGAAAGTGTATTGCATACCGAATTTAAAGTTAAGAAAACGCAAGGTAAT
    TACAATAATGAAATTGGTTTACCTTTAACTATTTTGGAATTAGATAATGATACTGAAATA
    TCAATATTGGAGATGGGGATGTCAGGTTTCCATGAAATTGAATTTCTGTCAAACCTCGCT
    CAACCAGATATTGCAGTTATAACTAATATTGGTGAGTCACATATGCAAGATTTAGGTTCG
    CGCGAGGGGATTGCTAAAGCTAAATCTGAAATTACAATAGGTCTAAAAGATAATGGTACG
    TTTATATATGATGGCGATGAACCATTATTGAAACCACATGTTAAAGAAGTTGAAAATGCA
    AAATGTATTAGTATTGGTGTTGCTACTGATAATGCATTAGTTTGTTCTGTTGATGATAGA
    GATACTACAGGTATTTCATTTACGATTAATAATAAAGAACATTACGATCTGCCAATATTA
    GGAAAGCATAATATGAAAAATGCGACGATTGCCATTGCGGTTGGTCATGAATTAGGTTTG
    ACATATAACACAATCTATCAAAATTTAAAAAATGTCAGCTTAACTGGTATGCGTATGGAA
    CAACATACATTAGAAAATGATATTACTGTGATAAATGATGCCTATAATGCAAGTCCTACA
    AGTATGAGAGCAGCTATTGATACACTGAGTACTTTGACAGGGCGTCGCATTCTAATTTTA
    GGAGATGTTTTAGAATTAGGTGAAAATAGCAAAGAAATGCATATCGGTGTAGGTAATTAT
    TTAGAAGAAAAGCATATAGATGTGTTGTATACGTTTGGTAATGAAGCGAAGTATATTTAT
    GATTCGGGCCAGCAACATGTCGAAAAAGCACAACACTTCAATTCTAAAGACGATATGATA
    GAAGTTTTAATAAACGATTTAAAAGCGCATGACCGTGTATTAGTTAAAGGATCACGTGGT
    ATGAAATTAGAAGAAGTGGTAAATGCTTTAATTTCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2073 [new locus tag: SACOL_RS10845 ]
  • symbol: MurF
  • description: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
  • length: 452
  • theoretical pI: 4.83726
  • theoretical MW: 50052.4
  • GRAVY: -0.236726

Function[edit source | edit]

  • reaction:
    EC 6.3.2.10?  ExPASy
    UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 452.8)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 111.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 93.9)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 79.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidbifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 53.8)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 48.5)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 16.5)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) 
    Cell Wall and Capsule - no subcategoryPeptidoglycan biosynthesis--gjo UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) 
  • PFAM:
    no clan definedMur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 153.6)
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 58.3)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 49.8)
    no clan definedHBS1_N; HBS1 N-terminus (PF08938; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL1274(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SACOL1759universal stress protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 18
    • Cmax: 0.12
    • Cmax_pos: 67
    • Smax: 0.175
    • Smax_pos: 17
    • Smean: 0.12
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEISILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDAYNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNALIS

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3] [4]
    quantitative data / protein copy number per cell: 200 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]