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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2079 [new locus tag: SACOL_RS10875 ]
  • pan locus tag?: SAUPAN005364000
  • symbol: cls2
  • pan gene symbol?: cls2
  • synonym:
  • product: cardiolipin synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2079 [new locus tag: SACOL_RS10875 ]
  • symbol: cls2
  • product: cardiolipin synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 2144652..2146136
  • length: 1485
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGATAGAGTTATTATCCATTGCACTCAAGCATTCTAATATTATTTTAAATTCAATATTT
    ATTGGTGCATTTATTTTAAACTTATTATTCGCCTTTACCATTATTTTCATGGAAAGACGT
    TCTGCCAATTCTATCTGGGCTTGGTTACTAGTCTTAGTTTTCTTGCCTTTATTCGGCTTC
    ATTTTATACTTACTATTAGGACGACAAATTCAACGTGACCAAATTTTCAAAATTGATAAG
    GAAGATAAAAAAGGATTAGAGTTAATCGTTGATGAGCAATTAGCTGCTTTAAAAAATGAA
    AACTTTTCAAATTCCAATTATCAAATTGTAAAATTTAAAGAAATGATTCAAATGTTGTTA
    TATAATAACGCAGCATTTTTAACAACAGACAACGATTTAAAAATATACACAGACGGCCAA
    GAAAAATTTGATGACCTAATACAAGACATCCGTAATGCTACTGATTATATTCATTTTCAG
    TACTATATTATTCAAAATGATGAATTAGGTCGTACCATTTTAAATGAACTTGGTAAAAAA
    GCGGAACAAGGTGTAGAAGTTAAAATTCTTTATGATGACATGGGTTCTCGTGGACTGCGT
    AAAAAAGGCTTACGCCCGTTTCGCAATAAAGGTGGACATGCTGAAGCATTTTTCCCATCA
    AAATTACCTTTAATTAACTTGCGTATGAACAATCGAAACCATCGAAAAATTGTTGTAATA
    GATGGGCAAATTGGATATGTTGGTGGTTTTAATGTTGGTGATGAGTACTTAGGTAAATCA
    AAAAAATTCGGCTATTGGCGAGATACGCATTTACGAATTGTCGGGGATGCAGTGAATGCA
    TTGCAATTACGATTTATTCTAGATTGGAATTCACAAGCCACACGTGACCACATCTCCTAT
    GATGATCGTTATTTCCCAGATGTAAATTCTGGTGGAACAATTGGCGTTCAAATAGCTTCT
    AGTGGTCCTGACGAAGAATGGGAACAGATTAAATACGGCTATTTGAAAATGATTTCATCT
    GCTAAAAAATCGATTTATATTCAATCTCCCTATTTCATACCTGATCAAGCCTTTTTAGAT
    TCTATTAAAATTGCGGCATTAGGTGGTGTTGATGTCAATATCATGATTCCTAATAAACCT
    GACCATCCGTTTGTTTTTTGGGCTACTTTAAAAAATGCAGCATCCTTATTAGATGCCGGT
    GTTAAAGTATTTCACTACGACAATGGCTTTTTACACTCAAAAACACTTGTTATAGATGAT
    GAAATTGCAAGTGTGGGAACAGCTAATATGGACCATCGCAGTTTCACATTGAATTTCGAA
    GTCAACGCTTTTATTTATGACCAACAAATTGCCAAAAAATTAAAACAAGCTTTTATAGAT
    GATTTAGCAGTATCTTCTGAATTAACAAAAGCACGTTATGCTAAGCGAAGTCTTTGGATT
    AAATTTAAAGAAGGTATTTCACAATTATTGTCACCTATCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2079 [new locus tag: SACOL_RS10875 ]
  • symbol: Cls2
  • description: cardiolipin synthetase
  • length: 494
  • theoretical pI: 8.49192
  • theoretical MW: 56517.7
  • GRAVY: -0.0979757

Function[edit source | edit]

  • reaction:
    EC 2.7.8.-?  ExPASy
    2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
  • TIGRFAM:
    cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 821.2)
    Genetic information processingProtein fateProtein folding and stabilizationcytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.9)
    MetabolismEnergy metabolismElectron transportcytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.9)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Cardiolipin synthetase (EC 2.7.8.-) 
  • PFAM:
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 157.1)
    PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 58.5)
    no clan definedPLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 43.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 11
    • Cmax: 0.128
    • Cmax_pos: 43
    • Smax: 0.165
    • Smax_pos: 6
    • Smean: 0.131
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Melody Tsai, Ryosuke L Ohniwa, Yusuke Kato, Sayaka L Takeshita, Toshiko Ohta, Shinji Saito, Hideo Hayashi, Kazuya Morikawa
Staphylococcus aureus requires cardiolipin for survival under conditions of high salinity.
BMC Microbiol.: 2011, 11;13
[PubMed:21241511] [WorldCat.org] [DOI] (I e)