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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
  • pan locus tag?: SAUPAN005402000
  • symbol: SACOL2103
  • pan gene symbol?: mnaA
  • synonym:
  • product: UDP-GlcNAc 2-epimerase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
  • symbol: SACOL2103
  • product: UDP-GlcNAc 2-epimerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2163782..2164909
  • length: 1128
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAAAGATTATGACCATATTTGGAACAAGACCCGAAGCCATAAAGATGGCTCCTTTA
    GTTAAAGCTTTGGAACAAGAAAAAATGCTCGAGCCAATTGTTGTTGTTACAGCACAGCAT
    AGAGAAATGCTTGATTCAGTATTAAGTACCTTTGAAATCAAACCAAAATACGATTTGAAT
    ATTATGAAATCAGGACAGACACTTTCTGAGATAACTTCTAAATCAATCACTCAATTAGAA
    CAAGTGATTCAATTAGAAAAACCAGACATGGTACTTGTACATGGTGATACGATGACGACA
    TTTGCAGGTGGTTTAGCGGCATTTTATAACCAAGTGCCGATTGGACACGTTGAAGCAGGG
    CTTAGAAGTTATGATAAATATTCACCTTTTCCAGAAGAGGTGAATAGACAATTAGTTGGT
    GTATTAGCTGACTTACATTTTGCACCTACTAAAAATGCTGCATCGCATTTGTTAAGTGAA
    GGGAAATATTCAGAAAGTGTTGTTGTCACTGGTAATACTGCGATTGATGCTATGAAATAT
    ACAGTTGATGACAATTATAAATCTAACATCATGGATAAATACCATGACAAAAAATTCATT
    CTTATGACAGCACACCGACGAGAAAATATCGGGAAACCCATGGAAAATATATTTAAAGCG
    GTTAGACGCTTAATAGATGAGTATACTGATTTAGCACTGGTATATCCAATGCATAAAAAC
    CCGAAAGTTCGAGAGGTGGCTCAAAAGATTTTAGGTAGTCATGACAGAATTGAATTGATT
    GAACCTTTGGACGTAGTAGATTTCCATAATTTTGCTAAAAAATCTTATTTTATTTTGACA
    GATTCAGGTGGAATTCAGGAAGAAGCGCCATCATTTAATAAGCCAGTTTTAGTTTTGCGA
    AGTGTTACAGAACGTCCTGAAGGTGTTGAAGCTGGCACATTAAAAGTCATTGGAACAAAT
    AAGCAAAATGTGTATCAAGCTGCAAAAGAATTAATCGATGATGAGAGATTATATCATCAA
    ATGTCTGAAGCATCTAATCCTTACGGTGATGGATTTGCTTCAGAAAGAATAGTTAATCAT
    ATTAAGTATTATTTGAATTTAATCACTGAAAAACCAAGCGATTTCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1128

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
  • symbol: SACOL2103
  • description: UDP-GlcNAc 2-epimerase
  • length: 375
  • theoretical pI: 6.52172
  • theoretical MW: 42412.4
  • GRAVY: -0.319733

Function[edit source | edit]

  • reaction:
    EC 5.1.3.14?  ExPASy
    UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesUDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 529.4)
    UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing (TIGR03568; EC 3.1.4.-,5.1.3.-; HMM-score: 122.5)
    putative cofactor-binding repeat (TIGR03807; HMM-score: 15.7)
    MetabolismEnergy metabolismAmino acids and aminesaspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 15)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharideslipid-A-disaccharide synthase (TIGR00215; EC 2.4.1.182; HMM-score: 13.9)
    conserved hypothetical protein (TIGR03492; HMM-score: 13.7)
  • TheSEED:  
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSialic Acid Metabolism UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14) 
  • PFAM:
    GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 410.3)
    Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 12.6)
    GT-A (CL0110) NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.181
    • Ymax_pos: 18
    • Cmax: 0.136
    • Cmax_pos: 24
    • Smax: 0.393
    • Smax_pos: 5
    • Smean: 0.312
    • D: 0.232
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 90 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]