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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2121 [new locus tag: SACOL_RS11095 ]
  • pan locus tag?: SAUPAN005424000
  • symbol: SACOL2121
  • pan gene symbol?:
  • synonym:
  • product: acetyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2121 [new locus tag: SACOL_RS11095 ]
  • symbol: SACOL2121
  • product: acetyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2181923..2182783
  • length: 861
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCTAAAGAACTTTGTTTTGAAGGTATCACTTTAAAAGCATTTGATGAACAATATCGT
    TCAGCAATTAATGATTTTGACTTGAATGAAAGACAACAAATATATTCATCTTTACCTAAA
    GAAGTTATTGATGATGCAATTAATGATGCTGATAGGATTGCTAACGTAGCAATAAACGAT
    AAAAATGAAGTGGTGGGCTTTTTTGTATTACATCGTTACTATCAGCATGAAGGTTATGAT
    ACACCTGAAAATGTCGTTTATATTCGTTCATTATCGATTAATGAAAAATATCAAGGTTTT
    GGATATGGCACGAAAATAATGATGTCATTGCCGCAATATGTTCAAGGTGTATTTCCTGAT
    TTTAATCATCTATATCTAGTAGTAGATGCGGAAAATGACAATGCTTGGAACCTATACGAA
    CGTGCTGGATTCATGCATACAGCGACTAAAGAAGAAGGACCAATTGGCAAAGAACGACTA
    TATTACTTGGACTTAGATTCAAAACATGTTTCATCATTAAAGCTTGAAGAAGAAAGTCGT
    TCAGAAGTGACCAATGTACATATCATTAATTTAATGATTGATGGCCAAAAGGTTGGCTTT
    ATCGCATTGGAGCAGATTGGTGAACGCATGAACATTGCTGCTATTGAAGTGGATAAATCA
    TATCGCTTTAATGGTATTGGTTCAAGTGCTCTGCGACAATTGCCAACTTACTTAAGAAAA
    AACTATGACAACCTTAATGTGATTACGATGATTCTGTTTGGAGAGAATAATGATTTTAAA
    CCATTATGTTTAAATAGTAATTTCGTTGAAATCGAACAAACTGATGATTATGTCGTTTTC
    GAAAAATATTTAAATTACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    861

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2121 [new locus tag: SACOL_RS11095 ]
  • symbol: SACOL2121
  • description: acetyltransferase
  • length: 286
  • theoretical pI: 4.45913
  • theoretical MW: 33110
  • GRAVY: -0.377273

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 58.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsmycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 26.4)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 18.8)
  • TheSEED:  
    spermine/spermidine acetyltransferase blt 
  • PFAM:
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 72.9)
    Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 46.5)
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 46.1)
    Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 29.5)
    Acetyltransf_15; Putative acetyl-transferase (PF17013; HMM-score: 25.1)
    FR47; FR47-like protein (PF08445; HMM-score: 24.5)
    Acetyltransf_CG; GCN5-related N-acetyl-transferase (PF14542; HMM-score: 22)
    GNAT_acetyltr_2; GNAT acetyltransferase 2 (PF13718; HMM-score: 18)
    GAF (CL0161) GAF_3; GAF domain (PF13492; HMM-score: 15.4)
    no clan definedPhage_RpbA; Phage RNA polymerase binding, RpbA (PF10789; HMM-score: 13.6)
    Acetyltrans (CL0257) Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 24
    • Cmax: 0.111
    • Cmax_pos: 24
    • Smax: 0.157
    • Smax_pos: 19
    • Smean: 0.076
    • D: 0.092
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVIDDAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGYGTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERLYYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVDKSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDDYVVFEKYLNY

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 63 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 18.97 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]