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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2122 [new locus tag: SACOL_RS11105 ]
  • pan locus tag?: SAUPAN005426000
  • symbol: SACOL2122
  • pan gene symbol?: coaA
  • synonym:
  • product: pantothenate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2122 [new locus tag: SACOL_RS11105 ]
  • symbol: SACOL2122
  • product: pantothenate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2183207..2184010
  • length: 804
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAGTTGGCATTGACGCTGGCGGTACACTAATTAAAATCGTTCAAGAGCAAGATAAC
    CAACGTACTTTTAAAACTGAATTAACTAAAAATATTGATCAAGTTGTGGAATGGTTAAAC
    CAACAGCAAATTGAAAAATTATGCTTAACTGGAGGTAATGCAGGTGTCATTGCTGAAAAC
    ATCAACATTCCTGCACAAATTTTTGTAGAGTTTGATGCTGCATCTCAAGGACTAGGTATT
    TTGTTAAAAGAGCAAGGCCATGACTTAGCAGATTATATTTTTGCAAATGTTGGTACCGGT
    ACGTCACTACATTATTTCGACGGCCAATCACAACGTCGTGTAGGCGGTATCGGTACAGGT
    GGAGGCATGATTCAAGGACTCGGTTATTTACTAAGTCAAATCACTGATTATAAACAGTTA
    ACTGACATGGCACAACACGGTGATCGTAACACTATCGATTTAAAGGTTAGACATATTTAT
    AAAGATACCGAACCACCCATTCCCGGTGATTTAACAGCAGCTAATTTTGGTCATGTACTA
    CATCATTTAGATGCTGATTTTACACCTAGCAATAAATTAGCAGCCGTTATTGGTGTCGTT
    GGCGAAGTTGTTACGACAATGGCCATTACTGTTGCACGTGAATTTAAAACTGAAAATATC
    GTTTATATCGGTTCTTCATTTCATAATAACGCTTTATTACGCAAAGTTGTGGAAGATTAT
    ACTGTGTTACGCGGTTGCAAACCGTACTATGTTGAAAACGGCGCATTTTCAGGTGCTATT
    GGTGCATTATATTTAGAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

Protein[edit source | edit]

Protein Data Bank: 5ELZ
Protein Data Bank: 5JIC

General[edit source | edit]

  • locus tag: SACOL2122 [new locus tag: SACOL_RS11105 ]
  • symbol: SACOL2122
  • description: pantothenate kinase
  • length: 267
  • theoretical pI: 5.55856
  • theoretical MW: 29096.8
  • GRAVY: -0.0988764

Function[edit source | edit]

  • reaction:
    EC 2.7.1.33?  ExPASy
    Pantothenate kinaseATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme Apantothenate kinase (TIGR00555; EC 2.7.1.33; HMM-score: 314.4)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 14.5)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 12.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsCoenzyme ACoenzyme A Biosynthesis Pantothenate kinase type II, eukaryotic (EC 2.7.1.33) 
  • PFAM:
    Actin_ATPase (CL0108) Fumble; Fumble (PF03630; HMM-score: 86.6)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 13.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 23
    • Cmax: 0.131
    • Cmax_pos: 23
    • Smax: 0.218
    • Smax_pos: 18
    • Smean: 0.1
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQIEKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTSLHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIYKDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTENIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 53 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL2122 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]