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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2151 [new locus tag: SACOL_RS11260 ]
  • pan locus tag?: SAUPAN005506000
  • symbol: glmM
  • pan gene symbol?: glmM
  • synonym:
  • product: phosphoglucosamine mutase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2151 [new locus tag: SACOL_RS11260 ]
  • symbol: glmM
  • product: phosphoglucosamine mutase
  • replicon: chromosome
  • strand: -
  • coordinates: 2222102..2223457
  • length: 1356
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGAAAATATTTTGGTACAGACGGAGTAAGAGGTGTCGCAAACCAAGAACTAACACCT
    GAATTGGCATTTAAATTAGGAAGATACGGTGGCTATGTTCTAGCACATAATAAAGGTGAA
    AAACACCCACGTGTACTTGTAGGTCGCGATACTAGAGTTTCAGGTGAAATGTTAGAATCA
    GCATTAATAGCTGGTTTGATTTCAATTGGTGCAGAAGTGATGCGATTAGGTATTATTTCA
    ACACCAGGTGTTGCATATTTAACACGCGATATGGGTGCAGAGTTAGGTGTAATGATTTCA
    GCCTCTCATAATCCAGTTGCAGATAATGGTATTAAATTCTTTGGATCAGATGGTTTTAAA
    CTATCAGATGAACAAGAAAATGAAATTGAAGCATTATTGGATCAAGAAAACCCAGAATTA
    CCAAGACCAGTTGGCAATGATATTGTACATTATTCAGATTACTTTGAAGGGGCACAAAAA
    TATTTGAGCTATTTAAAATCAACAGTAGATGTTAACTTTGAAGGTTTGAAAATTGCTTTA
    GATGGTGCAAATGGTTCAACATCATCACTAGCGCCATTCTTATTTGGTGACTTAGAAGCA
    GATACTGAAACAATTGGATGTAGTCCTGATGGATATAATATCAATGAGAAATGTGGCTCT
    ACACATCCTGAAAAATTAGCTGAAAAAGTAGTTGAAACTGAAAGTGATTTTGGGTTAGCA
    TTTGACGGCGATGGAGACAGAATCATAGCAGTAGATGAGAATGGTCAAATCGTTGACGGT
    GACCAAATTATGTTTATTATTGGTCAAGAAATGCATAAAAATCAAGAATTGAATAATGAC
    ATGATTGTTTCTACTGTTATGAGTAATTTAGGTTTTTACAAAGCGCTTGAACAAGAAGGA
    ATTAAATCTAATAAAACTAAAGTTGGCGACAGATATGTAGTAGAAGAAATGCGTCGCGGT
    AATTATAACTTAGGTGGAGAACAATCTGGACATATCGTTATGATGGATTACAATACAACT
    GGTGATGGTTTATTAACTGGTATTCAATTAGCTTCTGTAATAAAAATGACTGGTAAATCA
    CTAAGTGAATTAGCTGGACAAATGAAAAAATATCCACAATCATTAATTAACGTACGCGTA
    ACAGATAAATATCGTGTTGAAGAAAATGTTGACGTTAAAGAAGTTATGACTAAAGTAGAA
    GTAGAAATGAATGGAGAAGGTCGAATTTTAGTAAGACCTTCTGGAACAGAACCATTAGTT
    CGTGTCATGGTTGAAGCAGCAACTGATGAAGATGCTGAAAGATTTGCACAACAAATAGCT
    GATGTGGTTCAAGATAAAATGGGATTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2151 [new locus tag: SACOL_RS11260 ]
  • symbol: GlmM
  • description: phosphoglucosamine mutase
  • length: 451
  • theoretical pI: 4.38454
  • theoretical MW: 49265.2
  • GRAVY: -0.319734

Function[edit source | edit]

  • reaction:
    EC 5.4.2.10?  ExPASy
    Phosphoglucosamine mutaseAlpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanphosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
    MetabolismCentral intermediary metabolismAmino sugarsphosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
    MetabolismCentral intermediary metabolismAmino sugarsphosphoglucosamine mutase (TIGR03990; EC 5.4.2.10; HMM-score: 346.6)
    MetabolismEnergy metabolismSugarsphosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR01132; EC 5.4.2.2; HMM-score: 26.8)
    MetabolismEnergy metabolismOther4-oxalocrotonate tautomerase family enzyme (TIGR00013; EC 5.3.2.-; HMM-score: 13.3)
  • TheSEED:  
    Sialic Acid Metabolism Phosphoglucosamine mutase (EC 5.4.2.10) / FemD, factor involved in methicillin resistance 
    UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis Phosphoglucosamine mutase (EC 5.4.2.10) / FemD, factor involved in methicillin resistance 
  • PFAM:
    no clan definedPGM_PMM_I; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I (PF02878; HMM-score: 147.6)
    PGM_PMM_III; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III (PF02880; HMM-score: 114.1)
    PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 63.9)
    PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 57.7)
    Periplas_BP (CL0144) Peripla_BP_5; Periplasmic binding protein domain (PF13433; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 36
    • Cmax: 0.123
    • Cmax_pos: 24
    • Smax: 0.132
    • Smax_pos: 25
    • Smean: 0.07
    • D: 0.088
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPRVLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISASHNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQKYLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKCGSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQELNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVMMDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDVKEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLDK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 2116 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 61.37 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

L Jolly, F Pompeo, J van Heijenoort, F Fassy, D Mengin-Lecreulx
Autophosphorylation of phosphoglucosamine mutase from Escherichia coli.
J. Bacteriol.: 2000, 182(5);1280-5
[PubMed:10671448] [WorldCat.org] (P p)
S Wu, H de Lencastre, A Sali, A Tomasz
A phosphoglucomutase-like gene essential for the optimal expression of methicillin resistance in Staphylococcus aureus: molecular cloning and DNA sequencing.
Microb. Drug Resist.: 1996, 2(2);277-86
[PubMed:9158773] [WorldCat.org] [DOI] (P p)
L Jolly, S Wu, J van Heijenoort, H de Lencastre, D Mengin-Lecreulx, A Tomasz
The femR315 gene from Staphylococcus aureus, the interruption of which results in reduced methicillin resistance, encodes a phosphoglucosamine mutase.
J. Bacteriol.: 1997, 179(17);5321-5
[PubMed:9286983] [WorldCat.org] (P p)