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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02405
  • pan locus tag?: SAUPAN005506000
  • symbol: glmM
  • pan gene symbol?: glmM
  • synonym:
  • product: phosphoglucosamine mutase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02405
  • symbol: glmM
  • product: phosphoglucosamine mutase
  • replicon: chromosome
  • strand: -
  • coordinates: 2232836..2234191
  • length: 1356
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGAAAATATTTTGGTACAGACGGAGTAAGAGGTGTCGCAAACCAAGAACTAACACCT
    GAATTGGCATTTAAATTAGGAAGATACGGTGGCTATGTTCTAGCACATAATAAAGGTGAA
    AAACACCCACGTGTACTTGTAGGTCGCGATACTAGAGTTTCAGGTGAAATGTTAGAATCA
    GCATTAATAGCTGGTTTGATTTCAATTGGTGCAGAAGTGATGCGATTAGGTATTATTTCA
    ACACCAGGTGTTGCATATTTAACACGCGATATGGGTGCAGAGTTAGGTGTAATGATTTCA
    GCCTCTCATAATCCAGTTGCAGATAATGGTATTAAATTCTTTGGATCAGATGGTTTTAAA
    CTATCAGATGAACAAGAAAATGAAATTGAAGCATTATTGGATCAAGAAAACCCAGAATTA
    CCAAGACCAGTTGGCAATGATATTGTACATTATTCAGATTACTTTGAAGGGGCACAAAAA
    TATTTGAGCTATTTAAAATCAACAGTAGATGTTAACTTTGAAGGTTTGAAAATTGCTTTA
    GATGGTGCAAATGGTTCAACATCATCACTAGCGCCATTCTTATTTGGTGACTTAGAAGCA
    GATACTGAAACAATTGGATGTAGTCCTGATGGATATAATATCAATGAGAAATGTGGCTCT
    ACACATCCTGAAAAATTAGCTGAAAAAGTAGTTGAAACTGAAAGTGATTTTGGGTTAGCA
    TTTGACGGCGATGGAGACAGAATCATAGCAGTAGATGAGAATGGTCAAATCGTTGACGGT
    GACCAAATTATGTTTATTATTGGTCAAGAAATGCATAAAAATCAAGAATTGAATAATGAC
    ATGATTGTTTCTACTGTTATGAGTAATTTAGGTTTTTACAAAGCGCTTGAACAAGAAGGA
    ATTAAATCTAATAAAACTAAAGTTGGCGACAGATATGTAGTAGAAGAAATGCGTCGCGGT
    AATTATAACTTAGGTGGAGAACAATCTGGACATATCGTTATGATGGATTACAATACAACT
    GGTGATGGTTTATTAACTGGTATTCAATTAGCTTCTGTAATAAAAATGACTGGTAAATCA
    CTAAGTGAATTAGCTGGACAAATGAAAAAATATCCACAATCATTAATTAACGTACGCGTA
    ACAGATAAATATCGTGTTGAAGAAAATGTTGACGTTAAAGAAGTTATGACTAAAGTAGAA
    GTAGAAATGAATGGAGAAGGTCGAATTTTAGTAAGACCTTCTGGAACAGAACCATTAGTT
    CGTGTCATGGTTGAAGCAGCAACTGATGAAGATGCTGAAAGATTTGCACAACAAATAGCT
    GATGTGGTTCAAGATAAAATGGGATTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02405
  • symbol: GlmM
  • description: phosphoglucosamine mutase
  • length: 451
  • theoretical pI: 4.38454
  • theoretical MW: 49265.2
  • GRAVY: -0.319734

Function[edit source | edit]

  • reaction:
    EC 5.4.2.10?  ExPASy
    Phosphoglucosamine mutaseAlpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanphosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
    MetabolismCentral intermediary metabolismAmino sugarsphosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
    and 3 more
    MetabolismCentral intermediary metabolismAmino sugarsphosphoglucosamine mutase (TIGR03990; EC 5.4.2.10; HMM-score: 346.6)
    MetabolismEnergy metabolismSugarsphosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR01132; EC 5.4.2.2; HMM-score: 26.8)
    MetabolismEnergy metabolismOther4-oxalocrotonate tautomerase family enzyme (TIGR00013; EC 5.3.2.-; HMM-score: 13.3)
  • TheSEED:  
    Sialic Acid Metabolism Phosphoglucosamine mutase (EC 5.4.2.10) / FemD, factor involved in methicillin resistance 
    and 1 more
    UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis Phosphoglucosamine mutase (EC 5.4.2.10) / FemD, factor involved in methicillin resistance 
  • PFAM:
    no clan definedPGM_PMM_I; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I (PF02878; HMM-score: 147.6)
    and 4 more
    PGM_PMM_III; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III (PF02880; HMM-score: 114.1)
    PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 63.9)
    PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 57.7)
    Periplas_BP (CL0144) Peripla_BP_5; Periplasmic binding protein domain (PF13433; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 36
    • Cmax: 0.123
    • Cmax_pos: 24
    • Smax: 0.132
    • Smax_pos: 25
    • Smean: 0.07
    • D: 0.088
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPRVLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISASHNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQKYLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKCGSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQELNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVMMDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDVKEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLDK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

P Glanzmann, J Gustafson, H Komatsuzawa, K Ohta, B Berger-Bächi
glmM operon and methicillin-resistant glmM suppressor mutants in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 1999, 43(2);240-5
[PubMed:9925512] [WorldCat.org] (P p)