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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2159 [new locus tag: SACOL_RS11350 ]
  • pan locus tag?: SAUPAN005557000
  • symbol: SACOL2159
  • pan gene symbol?: sdrM
  • synonym:
  • product: drug transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2159 [new locus tag: SACOL_RS11350 ]
  • symbol: SACOL2159
  • product: drug transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2237711..2239054
  • length: 1344
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCGATTAAAGTCAATCATAACTGTTATAGCTTTAATATTAATTATGTTTATGTCAGCG
    ATTGAATCATCTATTATTTCGCTTGCATTACCTACAATTAAACAAGATTTAAATGCAGGA
    AATTTAATTTCATTAATTTTCACAGCGTATTTTATCGCATTAGTAATTGCCAATCCTATA
    GTGGGCGAATTACTTTCGAGATTTAAAATTATTTATGTGGCAATTGCAGGATTACTTTTA
    TTTAGTATAGGTAGTTTTATGTGTGGTTTAAGTACTAATTTTACTATGCTAATTATATCA
    CGCGTGATACAAGGTTTTGGATCAGGTGTATTGATGTCATTATCGCAAATTGTTCCAAAG
    TTAGCATTTGAAATTCCGTTACGATATAAAATTATGGGCATAGTTGGCAGTGTTTGGGGT
    ATTTCGAGTATCATTGGGCCACTTCTAGGTGGTGGTATATTAGAATTTGCAACATGGCAT
    TGGTTATTCTACATAAATATACCGATTGCGATCATTGCCATTATATTAGTTATATGGACA
    TTTCATTTTCCAGAGGAAGAAACAGTTGCTAAATCAAAATTTGATACTAAAGGGCTCACG
    CTATTTTATGTATTTATTGGATTGATTATGTTTGCTTTATTAAACCAACAGCTGTTACTT
    TTAAATTTCTTGAGTTTTATCTTAGCTATCGTTGTGGCAATGTGTTTATTTAAAGTAGAA
    AAACATGTTTCTTCACCATTTTTACCTGTGGTTGAATTTAATCGTTCGATTACTTTAGTT
    TTTATAACTGACCTTCTAACAGCTATTTGTTTAATGGGATTCAATTTATATATTCCAGTC
    TACCTTCAAGAACAACTAGGATTATCTCCATTGCAAAGTGGATTGGTTATTTTTCCTTTA
    TCTGTAGCTTGGATTACATTGAATTTTAATTTACATCGAATTGAAGCAAAACTATCAAGG
    AAAGTTATTTACTTACTATCATTTACATTGCTACTAGTAAGTAGTATTATCATTTCATTT
    GGTATTAAATTGCCGGTACTTATAGCATTTGTGTTAATTTTGGCAGGATTAAGTTTTGGA
    TATATTTATACGAAAGATAGTGTGATTGTCCAAGAGGAAACTAGCCCATTACAAATGAAG
    AAAATGATGTCATTTTATGGATTAACTAAAAATCTTGGGGCATCAATAGGTTCAACAATA
    ATGGGATATCTTTATGCGATACAATCAGGAATCTTTGGTCCAAACTTACACAATGTGTTA
    AGTGCTGTTGCTGTAATTAGCATTGGGCTTATTGTTTTATGGGTCGTATTTTTTAAAGAA
    CAGTCATCTCAATCAAAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1344

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2159 [new locus tag: SACOL_RS11350 ]
  • symbol: SACOL2159
  • description: drug transporter
  • length: 447
  • theoretical pI: 9.4822
  • theoretical MW: 49409.4
  • GRAVY: 1.08523

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
    multidrug resistance protein (TIGR00880; HMM-score: 72.1)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 58.9)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 52.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 38.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 31.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.6)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsH+ Antiporter protein (TIGR00900; HMM-score: 24.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 8.2)
  • TheSEED:  
    Uncharacterized MFS-type transporter 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 183.3)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 28)
    TRI12; Fungal trichothecene efflux pump (TRI12) (PF06609; HMM-score: 23.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.269
    • Ymax_pos: 30
    • Cmax: 0.228
    • Cmax_pos: 30
    • Smax: 0.56
    • Smax_pos: 5
    • Smean: 0.39
    • D: 0.316
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Youichi Yamada, Koh-ichiro Hideka, Sumiko Shiota, Teruo Kuroda, Tomofusa Tsuchiya
Gene cloning and characterization of SdrM, a chromosomally-encoded multidrug efflux pump, from Staphylococcus aureus.
Biol. Pharm. Bull.: 2006, 29(3);554-6
[PubMed:16508166] [WorldCat.org] (P p)