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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2382 [new locus tag: SACOL_RS12500 ]
  • pan locus tag?: SAUPAN005911000
  • symbol: SACOL2382
  • pan gene symbol?: gltT
  • synonym:
  • product: proton/sodium-glutamate symport protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2382 [new locus tag: SACOL_RS12500 ]
  • symbol: SACOL2382
  • product: proton/sodium-glutamate symport protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2441383..2442660
  • length: 1278
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCTCTATTCAAGAGAAAAATTAGCCTACCTATGCAAGTTGTTATTGCATTAGTGTTA
    GGTGTTGTCGTAGGACTTTTATTATATGGACAAGAAAACGTAGCAAATTACATTAAACCA
    TTTGGTGACGTATTTTTAAATTTAATTAAAATGATCGTTATACCAGTTGTATTTTGCTCA
    CTAGCGCTTTCTATTTCGAACGTTGGGGAATCGAAAACTGTAGGGCGTTATGGCTGGAAA
    ACAATTTTATACTTTGAAATTATTACAACAATCGCAATAGGTTTAGGGATTATCTTCGGT
    AACCTATTTAAACCAGGTGCTGGATTAGACCCAACAAAATTACCTAAAGGTGATATTTCT
    AAATATCAATCAACTGCACATGCAGCAGAACAATCTACATATGGAAATCATTTTATTGAT
    ACCATTGTACATATTATTCCGACAAACTTTTTTGAAGCTTTAAATAAGGGTGAATTATTA
    CCTATTATCTTCTTCGCAGTATTCTTTGGATTAGGATTAGCTGCTGTAGGTAAAAAAGCA
    GAACCAGTTAAAGAATTTTTAAGCGGATCGCTTGAAGCTGTGTTCTGGATGATTAATAAA
    ATTTTAAAATTAGCACCACTTGGAGTGTTTGCATTCATTTGTACTACAATTATTACATTT
    GGTGCATCCGCATTATTACCACTATTAAAATTAGTATTAGTTGTTGTCTTTGCAATGGTG
    TTCTTTGTATTCGCTATACTAGGACTAGTTGCATGGATGTGTGGTATTAATATCATGAAT
    ATTATTAGAATCTTGAAAAGTGAATTGCTTTTAGCATTTTCTACATCAAGTTCGGAAGCT
    GTACTTCCTGTAATGATGAAGAAAATGGAAAACTTCGGTTCTCCAAAAGAAATTACTTCT
    TTTGTTATACCAATTGGTTATACGTTTAACTTAGATGGATCAGCACTTTATCAATCTATT
    GCAGCATTATTCGTTGCACAGATGTATGGAATGCACTTAACATTATCAGAGCAAATTGTG
    TTGATGTTAACATTAATGATTACGTCTAAAGGTATGGCGGCAGTACCAGGAACTTCAATC
    GTTGTTTTATTAACAACATTAGGTGCCATGGGCTTACCGGCACAAGGTTTAGCATTAATT
    ATTGGTGTTGACCGTATCTTAGATATGGTACGTACATGTGTAAACGTTATTGGTAATGCA
    TTATCAACAATCGTTATAGCTAAATGGGAAAACGTATATGACAAAGCAAAAGGTCAAGAA
    TATTTAAAATCAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2382 [new locus tag: SACOL_RS12500 ]
  • symbol: SACOL2382
  • description: proton/sodium-glutamate symport protein
  • length: 425
  • theoretical pI: 9.36462
  • theoretical MW: 46068.2
  • GRAVY: 0.874118

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Proton/glutamate symport protein 
    Sodium/glutamate symport protein 
  • PFAM:
    no clan definedSDF; Sodium:dicarboxylate symporter family (PF00375; HMM-score: 410)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.297
    • Ymax_pos: 18
    • Cmax: 0.174
    • Cmax_pos: 38
    • Smax: 0.707
    • Smax_pos: 8
    • Smean: 0.534
    • D: 0.389
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNLFKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELLPIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTSSSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLTLSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]