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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2582 [new locus tag: SACOL_RS13525 ]
  • pan locus tag?: SAUPAN006232000
  • symbol: SACOL2582
  • pan gene symbol?: oatA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2582 [new locus tag: SACOL_RS13525 ]
  • symbol: SACOL2582
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2645664..2647475
  • length: 1812
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATACAAAAGACTTTAAACGTTTAGAAAAAATGTATTCCCCGCGATACTTACCTGGA
    TTAGATGGATTGAGGGCATTCGCAGTTATAGGAATCATTATTTATCACTTGAATGCACAA
    TGGTTAAGTGGGGGCTTTTTAGGAGTAGATACATTCTTCGTTATTTCAGGTTATTTAATA
    ACAAGTTTGTTGATAAGTGAGTATTATCGGACGCAAAAAATCGATTTGCTAGAGTTTTGG
    AAGCGACGATTGAAACGACTCATTCCGGCAGTGTTATTTTTAATCTGTGTCGTGCTTACG
    TTCACACTGATATTTAAACCGGAATTGATTATACAAATGAAACGAGATGCTATTGCAGCT
    ATATTCTATGTTTCAAACTGGTGGTACATTTCACAGAATGTAGATTATTTTAACCAATTT
    GCTATTGAACCACTCAAACATTTATGGTCTTTAGCCATTGAAGAACAATTTTACTTGCTT
    TTCCCATTGGTTATCACGTTCTTATTACATAGATTTAAACCGAGAAATATTATTCAAACG
    CTATTTATTGTATCGTTGATTTCTTTAGGACTTATGATAGTGATTCATTTCATCACTGGA
    GATAATTCACGTGTGTATTTTGGGACAGATACACGACTGCAAACTTTATTGCTTGGTTGT
    ATATTAGCATTTATTTGGCCTCCGTTTGCTTTGAAAAAAGATATTTCTAAAAAGATTGTC
    GTATCATTAGATATTATAGGGATATCTGGTTTTGCGGTTCTAATGACTTTGTTCTTTATA
    GTTGGAGACCAAGATCAATGGATCTATAATGGAGGATTTTACATTATATCATTTGCAACT
    TTATTCATTATTGCAATTGCGGTACATCCTTCTAGTTTATTTGCTAAATTTTTAAGTATG
    AAACCTTTACTAATTATAGGTAAACGATCATATAGCTTATACTTATGGCATTATCCTATC
    ATTGTTTTTGTGAACAGTTATTACGTACAAGGACAAATACCGGTATACGTTTATATTATA
    GAAATTTTGTTAACAGCGTTAATGGCTGAAATTTCGTATCGCTTTATTGAAACACCTATA
    CGTAAAAAAGGATTTAAAGCTTTTGCATTTTTACCTAAAAAGAAGGGGCAATTTGCTAGA
    ACAGTGTTAGTTATCCTATTATTGGTTCCGTCTATCGTTGTGCTCAGTGGACAGTTTGAT
    GCACTTGGCAAACAACATGAAGCCGAGAAGAAAGAGAAGAAGACGGAATTTAAAACAACG
    AAGAAAAAAGTCGTTAAAAAAGATAAGCAAGAGGATAAGCAGACAGCGAATAGCAAAGAG
    GATATTAAAAAGTCATCACCACTATTAATTGGTGACTCGGTCATGGTGGATATTGGTAAT
    GTCTTTACTAAGAAAATACCAAATGCACAAATTGATGGTAAAGTTGGACGGCAACTCGTT
    GATGCTACACCAATTGTGAAATCGCAATATAAAGACTATGCTAAAAAAGGTCAAAAAGTT
    GTAGTAGAGCTTGGTACAAATGGGGCATTTACGAAAGATCAATTAAATGAACTATTGGAT
    AGTTTTGGAAAAGCAGACATATATTTAGTTTCTATTAGAGTACCTAGAGATTATGAAGGT
    AGAATAAATAAATTAATTTATGAGGCAGCTGAAAAGCGCTCTAATGTACATCTAGTCGAT
    TGGTATAAAGCTTCTGCAGGTCATCCGGAATACTTTGCATATGACGGTATTCACTTAGAA
    TATGCAGGTAGTAAAGCGCTGACTGATTTGATTGTAAAAACGATGGAAACACATGCTACA
    AATAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1812

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2582 [new locus tag: SACOL_RS13525 ]
  • symbol: SACOL2582
  • description: hypothetical protein
  • length: 603
  • theoretical pI: 10.0029
  • theoretical MW: 69098.1
  • GRAVY: 0.222554

Function[edit source | edit]

  • reaction:
    EC 2.3.1.-?  ExPASy
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationstage III sporulation protein AF (TIGR02896; HMM-score: 5.1)
  • TheSEED:  
    Acyltransferase 
  • PFAM:
    Acyl_transf_3 (CL0316) Acyl_transf_3; Acyltransferase family (PF01757; HMM-score: 93.7)
    SGNH_hydrolase (CL0264) Lipase_GDSL_2; GDSL-like Lipase/Acylhydrolase family (PF13472; HMM-score: 16.9)
    no clan definedDisA-linker; DisA bacterial checkpoint controller linker region (PF10635; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.108
    • Ymax_pos: 11
    • Cmax: 0.121
    • Cmax_pos: 42
    • Smax: 0.148
    • Smax_pos: 1
    • Smean: 0.113
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSGGFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFTLIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLLFPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLLGCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYIISFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIPVYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSIVVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLIGDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNGAFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASAGHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Agnieszka Bera, Silvia Herbert, Andreas Jakob, Waldemar Vollmer, Friedrich Götz
Why are pathogenic staphylococci so lysozyme resistant? The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus.
Mol. Microbiol.: 2005, 55(3);778-87
[PubMed:15661003] [WorldCat.org] [DOI] (P p)